Protein Alignment AGO1 and Ago3

DIOPT Version :10

• Sequence 1: NP_725341.1
• Gene: AGO1 / 36544
• FlyBaseID: FBgn0262739
• Length: 984
• Species: Drosophila melanogaster
• Sequence 2: NP_700451.2
• Gene: Ago3 / 214150
• MGIID: 2446634
• Length: 860
• Species: Mus musculus
• Alignment Length: 864
• Identity: 622/864 - (71%)
• Similarity: 713/864 - (82%)
• Gaps: 26/864 - (3%)

  Fly   131 PVFTCPRRPNLGREGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVNREIIETMV-HAYSK 194
            |:|..||||..|..|:||.|.||.|||.:|:..|:.|:::|:||||||:||||::::|| |....
Mouse    13 PLFIVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVT 77

  Fly   195 IFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGE-GKDRIFRVTIKWQAQVSLFNLEEALEG 258
            |||..:||:||:.:|||.:|||:....::|:|||||| ||||.|:|::|:.::||...|.|||.|
Mouse    78 IFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSVKFVSRVSWHLLHEALAG 142

  Fly   259 RT--------RQIPYDAILALDVVMRHLPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSV 315
            .|        :.:..:.:.|:|||:||||||.||||||||||:||||.|||||||||||||||||
Mouse   143 GTLPEPLELDKPVSTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSV 207

  Fly   316 RPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITH 380
            ||:.|||||||||||||||||||||.||||||||.:|:||.:|||||.||||||||||||:|:||
Mouse   208 RPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTH 272

  Fly   381 CGQMRRKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQVGQEHKH 445
            ||.|||||||||||||||..|:||||||||||||.|||:||.:||.::|:||||||||||||.||
Mouse   273 CGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKH 337

  Fly   446 TYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREINNLVKRADFNNDSYVQEFGLTI 510
            ||||||||||||||||||||||.||||||||||||||||:.||:.||:.|::..|.:||||...:
Mouse   338 TYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKV 402

  Fly   511 SNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRIW 575
            .:.|..|.|||||.|.||||||..|               :|:|:.||||||||||.|||||::|
Mouse   403 RDEMAHVTGRVLPAPMLQYGGRNRT---------------VATPSHGVWDMRGKQFHTGVEIKMW 452

  Fly   576 AIACFAPQRTVREDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFPGLQ 640
            ||||||.||..||:.|:.||.||:|||.||||||.|||||||||.|.|.||||||:||.|:.|||
Mouse   453 AIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQ 517

  Fly   641 LVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNVNKTSPQTLSNLCLKINVKLGGINSILVPS 705
            |::|:|||||||||||||||||:|||||||||.|||.||||||||||||||||||||||:||||.
Mouse   518 LIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPH 582

  Fly   706 IRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIIQELSSMV 770
            .||.||.:||||||||||||||||.|||||||||||||||||||.||||||:.||||||:|:|||
Mouse   583 QRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMV 647

  Fly   771 RELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKR 835
            |||||.||||| .:||.|||.||||||||||..||.:||.|||||||.||.:|:||||:||||||
Mouse   648 RELLIQFYKST-RFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKR 711

  Fly   836 HHTRLFCAEKKEQSGKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFD 900
            ||||||||::.|:.|:|||||||||||..||||.|||||||||.||||||||||||||||||.|.
Mouse   712 HHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFT 776

  Fly   901 SDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSHQSGCSEDRTPG 965
            :||||.|||||||||||||||||||||||||||||||||||||:|||||.||||.||.|..|.|.
Mouse   777 ADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQ 841

  Fly   966 AMARAITVHADTKKVMYFA 984
            |:|:|:.:|.||.:.||||
Mouse   842 ALAKAVQIHQDTLRTMYFA 860

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AGO1	NP_725341.1	ArgoN	146..275	CDD:465134	63/138 (46%)
		ArgoL1	285..335	CDD:462567	45/49 (92%)
		PAZ_argonaute_like	336..456	CDD:239212	97/119 (82%)
		Piwi_ago-like	502..942	CDD:240015	336/439 (77%)
Ago3	NP_700451.2	ArgoN	73..167	CDD:465134	39/93 (42%)
		ArgoL1	177..227	CDD:462567	45/49 (92%)
		PAZ_argonaute_like	228..348	CDD:239212	97/119 (82%)
		Piwi_ago-like	393..818	CDD:240015	336/440 (76%)
		Interaction with guide RNA. /evidence=ECO:0000250|UniProtKB:Q9H9G7	530..567		33/36 (92%)
		Interaction with guide RNA. /evidence=ECO:0000250|UniProtKB:Q9H9G7	758..805		42/46 (91%)