Protein Alignment shot and Sptbn2

DIOPT Version :10

• Sequence 1: NP_725339.1
• Gene: shot / 36542
• FlyBaseID: FBgn0013733
• Length: 8805
• Species: Drosophila melanogaster
• Sequence 2: XP_008758314.1
• Gene: Sptbn2 / 29211
• RGDID: 3751
• Length: 2395
• Species: Rattus norvegicus
• Alignment Length: 2534
• Identity: 529/2534 - (20%)
• Similarity: 915/2534 - (36%)
• Gaps: 661/2534 - (26%)

  Fly   208 RREISDIVVGKEDN---VSAREALLRWARRSTARYPGVRVNDFTSSWRDGLAFSALVHRNRPDLL 269
            |.:|.||.|..|||   .||::|||.|.:..||.||.|.|::||:||||||||:|:||::|||||
  Rat   166 RFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDLL 230

  Fly   270 DWRKARNDRPRERLETAFHIVEKEYGVTRLLDPEDVDTNEPDEKSLITYISSLYDVFP------- 327
            |:...:.......|:.||::.|||.|:|:|||||||:.::|||||:|||:::.|..|.
  Rat   231 DFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYFSKMKALAV 295

  Fly   328 EPPSIHPLFD--MESQRRVHEYRDLAQQFIYWCREKTAYLQERSFPPTLIEMKRLLSDLQRFRSD 390
            |...|..:.|  ||::..|.:|..||.:.:.|..:....|.:|....:|..::..|.....:|:.
  Rat   296 EGKRIGKVLDHAMEAEHLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSYRTV 360

  Fly   391 EVSARKREKSK----LIQIYKELERYFETVGEVDVEAELRPDAIEKAWYRMNTALQDREVILQQE 451
            |...:..||..    |..|..:|....:.| ....|..|..| |.|||.|:..|..:||:.|:.|
  Rat   361 EKPPKFTEKGNLEVLLFTIQSKLRANNQKV-YTPREGRLISD-INKAWERLEKAEHERELALRTE 423

  Fly   452 IERLERLQRLADKVQREIKHVDQKLTDLEGRIGEEGRRIERLHPVDAKSIVEALETEIRHLEEPI 516
            :.|.|:|::||.:..|:....:..|::.:..:.::...:|......|....||:||:|......:
  Rat   424 LIRQEKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRV 488

  Fly   517 QDMNQDCHVLNEGRYPHVSELHKKVNKLHQRWAQLRTNFHTNLVQKLSGLKYPVHETTVTRQTRM 581
            |.::.....|....|..:..:..:.|.:.:.|..||                   |....|:.|:
  Rat   489 QAVDAVAAELAAEHYHDIKRIAARQNNVARLWDFLR-------------------EMVAARRERL 534

  Fly   582 VVESRQIDTNPHFRDLQEHIEWCQNKLKQLLAADYGSDLPSVKEELDRQQHEHKIIDQFHTKILN 646
            ::   .::....|:||...::|......:|.:.|.|..|..|::.|           |.|..:..
  Rat   535 LL---NLELQKVFQDLLYLMDWMAEMKGRLQSQDLGKHLAGVEDLL-----------QLHELVEA 585

  Fly   647 D-----ERQQT-------------KFSGDELALYQQRLNQLQKVY---AELLSTSTKRLSDLDSL 690
            |     ||.:.             ::...:..|..:|:..|::.|   .||.:|...||.:...|
  Rat   586 DIAVQAERVRAVSASALRFCDPGKEYRPCDPQLVSERVATLEQSYEALCELAATRRARLEESRRL 650

  Fly   691 QHFLGQASAELQWLNEKEQVEITRDWADKQLDLPSVHRY---YENLMSELEKREMHFATILDRGE 752
            ..||.:......|:.|::.:..:   |:...||..|.|.   :..|..|:..|.......|::|:
  Rat   651 WRFLWEVGEAEAWVREQQHLLAS---AETGRDLTGVLRLLNKHTALRGEMSGRLGPLKLTLEQGQ 712

  Fly   753 ALLNQQHPASKCIEAHLTALQQQWAWLLQLTLCLEVHLKHATEYHQFFGEIKDAEQWLAKRDEIL 817
            .|:.:.||.:.........||.||..|..|.......|..|...:||..:..|.|.||.....::
  Rat   713 QLVAEGHPGANQASTRAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLV 777

  Fly   818 NSKFSQSDFGLDQGET--LLRGMQDLREELNAFGETVATLQRRAQTVVPLNKRRQPVNRQGPVQA 880
            :|    .:.|.|:..|  |.|..:.|.||:.|...|:..|:.:|..:.|.......|  ||.|..
  Rat   778 SS----PEVGHDEFSTQALARQHRALEEEIRAHRPTLDALREQAAALPPALSHTPEV--QGRVPT 836

  Fly   881 ICAYKQQGQLQIEKGETVTLLDNSGRVKWRVRTAKGQEGPIPGACLLLPPPDQEAIDAAERLKRL 945
            :..:.:  :||...||....|:.:......:..|        |||.|.....::.::.....:||
  Rat   837 LEQHYE--ELQARAGERARALEAALAFYTMLSEA--------GACGLWVEEKEQWLNGLALPERL 891

  Fly   946 FDRSVALWQKKHLRLRQNMIFATIRVVKGWDFDQFLAMGPEQRTAIRR--ALNDDADKLLSEGDP 1008
            .|                     :.||:    .:|..:.||......|  |::|.|::||....|
  Rat   892 ED---------------------LEVVQ----QRFETLEPEMNALAARVTAVSDIAEQLLKASPP 931

  Fly  1009 NDPQLRRLRREMDEVNRLFDEFEKRA---RAEEESKQASRIFTEECLAIKSKLEDMARELDQIIL 1070
            ...   |:....:::|:.:.:|...|   :|...|..:.:.:..||...::.:.:..:.::.
  Rat   932 GKD---RIIGTQEQLNQRWQQFRSLADGKKAALTSALSIQNYHLECTETQAWMREKTKVIES--- 990

  Fly  1071 APLPRDL-DSLEHVLEIH---SDYERRLHLLEPELKHLQETFRTIA----LKTPVLKKSLDNLME 1127
               .:|| :.|..||.:.   :..||.|..:...:..|.:....:|    .:.|.:...|..:..
  Rat   991 ---TQDLGNDLAGVLALQRKLAGTERDLEAISARVGELTQEANALAAGHPAQAPAINTRLGEVQT 1052

  Fly  1128 LWKELNT----------QSGLHKDRLKLLEASLAGL---------EDNEHVISELENELARHQDL 1173
            .|::|..          ::...:|.|:.|:...|.|         |:....:.|.|..||:|..|
  Rat  1053 GWEDLRATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAAL 1117

  Fly  1174 PSTAEGLQQVFKQLNHMQDIITQQQPQMDKMNDAADQLGRMGVPTKVLGDLKRLHSNVERLNTRW 1238
            ....|..|..:.:|..:.:.:|:         |.||       |..:.     |...:|.|.|.|
  Rat  1118 RGEVERAQSEYSRLRTLGEEVTR---------DQAD-------PQCLF-----LRQRLEALGTGW 1161

  Fly  1239 SAVCNQLGERMRSCETAIGLMKNLQSSVQVE-----ESWVDGTTERLSAMPTATSAY-ELDKLLG 1297
            ..:......|......|.|....|:.:.|.|     :.:|...||....:..|.:|. :|:..:.
  Rat  1162 EELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSSQEYVLSHTEMPGTLQAADAAIKKLEDFMS 1226

  Fly  1298 AAIERKPKIENVNVAGGRLIREAKIYDSKCLRFVDWLVEARPSFSPPRRDLRPADSDPGATQYYS 1362
            .......:|..:..||.:|:.:..|:..|        ::.:            |||
  Rat  1227 TMDANGERIRGLLEAGRQLVSKGNIHAEK--------IQEK------------ADS--------- 1262

  Fly  1363 QRLDNLNTKNDRLLEQLSQRLKTAIEVNGSDGLQYAESLQ--KPLKTFRVDFSAGSVPTGDGYAA 1425
              ::..:.||...::||..||:        |..:....||  :.||.: :|
  Rat  1263 --IEKRHRKNQEAVQQLLGRLR--------DNREQQHFLQDCQELKLW-ID-------------- 1302

  Fly  1426 RQEDLYTTTYSTTQISSTKTTKSSTKSVYSSDGLDAASQEVSTAGLPQSQIQFNEIRTLKRSQQL 1490
              |.:.|                              :|:||          ::|.|.|....|.
  Rat  1303 --EKMLT------------------------------AQDVS----------YDEARNLHTKWQK 1325

  Fly  1491 GGHSVLDIAGIRD-----PRTGRVLTIGEAIQLRILDVRTGEMLVG-----DRRITLEQAADQGL 1545
            ....:.::|..:|     .:.||.||: |..:|::|   ..|.|..     |...|..||..:.|
  Rat  1326 HQAFMAELAANKDWLDKVDKEGRELTL-EKPELKVL---VSEKLEDLHRRWDELETTTQAKARSL 1386

  Fly  1546 IDLQLAK------------------QLLEPGAGRDASGRELSL--LEVIQREISEAESGYETAEK 1590
            .|...|:                  ||.....|:|.:...:.|  .::::||::..|...|..:.
  Rat  1387 FDANRAELFAQSCSALESWLESLQAQLHSDDYGKDLTSVNILLKKQQMLEREMAVREKEVEAIQA 1451

  Fly  1591 RIKVNNTVTVEQTSGELGSPENPRNIADAITAGSVDTKTGLYRVKSG------------------ 1637
            :.|.  ....:|::||:  ....|.:.:...|.....|....|:::.
  Rat  1452 QAKA--LAQEDQSAGEV--ERTSRAVEEKFRALCQPMKDRCRRLQASREQHQFHRDVEDEILWVT 1512

  Fly  1638 QTISLAEAYERGYLIRHESVTIKSNALCLSDAIAHGLVDGAGWIADRNSGDKFRLDSAIANQLID 1702
            :.:.:|.:.|.|..:....:.:|.|.....:...|     ...|||.....:....:|...:|
  Rat  1513 ERLPMASSLEHGKDLPSVQLLMKKNQTLQKEIQGH-----EPRIADLKERQRTLGTAAAGPEL-- 1570

  Fly  1703 ASVREV---------VDAKRDTKITLQEALQ-------SGVLNAKTGRYV-----NEVTKEKLTF 1746
            |.::|:         :..||     |:|||:       :....|..|...     .|..|::|:.
  Rat  1571 AELQEMWKRLSHELELRGKR-----LEEALRAQQFYRDAAEAEAWMGEQELHMMGQEKAKDELSA 1630

  Fly  1747 -AEARNRQLIVK-----PYTLKDIC--DLNLLDKQAQITSPMRREKLSIMQA----IEAGV---- 1795
             ||.:..|::.:     ..|:|.:.  ..:::|.:...::     :|:|.||    :.||:
  Rat  1631 QAEVKKHQVLEQALADYAQTIKQLAASSQDMIDHEHPEST-----RLTIRQAQVDKLYAGLKELA 1690

  Fly  1796 ------LDGNLLKCITKRKGELVTLQEAIADGIVLPA--------------ECKYRDF------M 1834
                  |..:|..|..:|  ||..|::.|.:..|:.|              ..|:|:|      :
  Rat  1691 GERRERLQEHLRLCQLRR--ELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTI 1753

  Fly  1835 TGELISIPEAVERGLISS--VAQRSIFDIDGFKDLRSNDYVSFNVALSRDLLRRKSTGFALETGR 1897
            ..|.:....|:..|||:.  .|:.::.:   :||       |.|.|.: ||
  Rat  1754 GQERVDSANALANGLIAGGHAARATVAE---WKD-------SLNEAWA-DL-------------- 1793

  Fly  1898 DSLVPLEVAVSEGLVRQEVYEMFSRGIGVQNASGKELSVFDLVYHNLIDPKTGYLLDPKTGETVP 1962
                 ||:..:.|.|....||:.....|.:.|..:       |.|     |...|.| .||..:.
  Rat  1794 -----LELLDTRGQVLAAAYELQRFLHGARQALAR-------VQH-----KQQQLPD-GTGRDLN 1840

  Fly  1963 LDTAIERKFITPEGALLLSSLLNITLTTETVTRTINRYVTIRAGSQ-EPVDSVLLTFTE--AVRQ 2024
            ...|::|:....|     ..:..::...:.|.....|.....||.: |.:...:....|  |..|
  Rat  1841 AAEALQRRHCAYE-----HDIQALSTQVQQVQDDGLRLQKAYAGDKAEEIGRHMQAVAEAWAQLQ 1900

  Fly  2025 GFIDEERQLFKDPKTGNIYSVQQALNYGLLVPDSNQTVPEPTNRKKTKSTITIVTKQIIPEAEPI 2089
            |.....|||..|  |.:.:...:|:...:|..|......:...|.:..|:..:|.|
  Rat  1901 GSSAARRQLLLD--TTDKFRFFKAVRELMLWMDGINLQMDAQERPRDVSSADLVIK--------- 1954

  Fly  2090 KLNTQHTK-YVEKSVE-----IPISQELVKPHRVVSEFINLEKSSYIE---QNVTERQIMELPPG 2145
              |.|..| .:|...:     |.:.|||:..:...:|.|: ||.|.::   |...|:
  Rat  1955 --NQQGIKAEIEARADRFSACIDMGQELLARNHYAAEEIS-EKLSQLQSRRQETAEK-------- 2008

  Fly  2146 GWR-----LKDAIEQRLFNPDTGVFHVQGTDRLVNFEECINKQIINNLSLSVIDPNTGDKISVQS 2205
             |:     |:..:|..:|..|.|         :.....|..:.::.:..|..........|....
  Rat  2009 -WQEKMDWLQLVLEVLVFGRDAG---------MAEAWLCSQEPLVRSAELGCTVDEVESLIKRHE 2063

  Fly  2206 AFERD---------------ILDSYGNYTNSRKQVQGMR---------------SAIDESKIILE 2240
            ||::.               .|:...|....:::.:..|               |.:|..: :|:
  Rat  2064 AFQKSAVAWEERFSALEKLTALEERENEQKRKREEEERRKQPPTSEPMASQPEGSLVDGQR-VLD 2127

  Fly  2241 TVPATRGANQKTILRITKVNNIPDVLEVSTPLKDAPPKFVEVLTCQRELAS--PEPLQIAPGAIY 2303
            |  |..|...|    :......|.:..|.|..:.:.|    :|..||...|  ||    .||
  Rat  2128 T--AWDGTQSK----LPPSTQAPSINGVCTDTESSQP----LLEQQRLEQSNVPE----GPG--- 2175

  Fly  2304 DPSTALVIFTQTGETENIFDAARQGLVDEQLIKIVDPTTKQPISVTEAIARSIYDPKTATILDSE 2368
                     :.||:..:.....||.|          |....|..:.:  :||......||
  Rat  2176 ---------SGTGDESSGPRGERQTL----------PRGPAPSPMPQ--SRSSESAHVAT----- 2214

  Fly  2369 GQPVDLITATKLGLLSVVGAPLVA---AEGAL---------------------------RTVRFV 2403
                          |...||.|.|   .||.|                           .::.|.
  Rat  2215 --------------LPARGAELSAQEQMEGTLCRKQEMEAFNKKAANRSWQNVYCVLRRGSLGFY 2265

  Fly  2404 TDPRTGE-------QIPVEVAYERGIVSRDQLHRGRSFDSEPATVEDKVVVLQRMRKVILKPKD 2460
            .|.|...       ::||.:|..:|.|:.|...|...|.          :.||..::.:.:.||
  Rat  2266 KDARAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFK----------LGLQDGKEYLFQAKD 2319

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
shot	NP_725339.1	SAC6	204..>420	CDD:227401	86/227 (38%)
		CH_PLEC-like_rpt2	223..327	CDD:409038	55/103 (53%)
		SPEC	471..689	CDD:238103	42/238 (18%)
		SPEC	690..866	CDD:238103	46/180 (26%)
		SH3_10	864..929	CDD:407754	15/64 (23%)
		SPEC	1047..1250	CDD:238103	42/229 (18%)
		SPEC	1153..1390	CDD:238103	48/251 (19%)
		PLEC	<1498..1526	CDD:197605	9/32 (28%)
		Plectin	1949..1980	CDD:459901	7/30 (23%)
		DUF5585	2860..>3198	CDD:465521
		SMC_prok_B	<4936..>5564	CDD:274008
		SPEC	5304..5513	CDD:238103
		SPEC	<5658..5787	CDD:413338
		SPEC	5793..6007	CDD:238103
		SPEC	6011..6220	CDD:238103
		SPEC	6118..6330	CDD:238103
		PRK09039	<6279..6441	CDD:181619
		SPEC	6443..6660	CDD:238103
		SPEC	6663..6878	CDD:238103
		SPEC	6880..7087	CDD:238103
		SPEC	6986..7196	CDD:238103
		SPEC	7196..7417	CDD:238103
		SPEC	7314..7526	CDD:238103
		SPEC	7528..7747	CDD:238103
		SPEC	7749..7968	CDD:238103
		SPEC	7973..8181	CDD:238103
		FRQ1	<8375..8444	CDD:444056
		GAS2	8450..8522	CDD:128539
		PHA03307	8550..>8795	CDD:223039
Sptbn2	XP_008758314.1	CH_SPTB-like_rpt1	52..166	CDD:409095	529/2534 (21%)
		CH_SPTBN2_rpt2	180..298	CDD:409170	57/117 (49%)
		Spectrin	312..421	CDD:395348	28/110 (25%)
		Spectrin	436..529	CDD:395348	17/111 (15%)
		SPEC	541..753	CDD:238103	49/225 (22%)
		SPEC	756..965	CDD:238103	55/252 (22%)
		SPEC	968..1179	CDD:238103	42/237 (18%)
		SPEC	1181..1390	CDD:238103	56/308 (18%)
		SPEC	1391..1595	CDD:238103	35/219 (16%)
		SPEC	1596..1808	CDD:238103	47/248 (19%)
		SPEC	1809..2017	CDD:238103	50/248 (20%)
		SPEC	2025..>2082	CDD:197544	8/65 (12%)
		PH_beta_spectrin	2228..2331	CDD:269975	18/102 (18%)