DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment stj and Cacna2d3

DIOPT Version :9

Sequence 1:	NP_001246303.1	Gene:	stj / 36526	FlyBaseID:	FBgn0261041	Length:	1271	Species:	Drosophila melanogaster
Sequence 2:	NP_033915.1	Gene:	Cacna2d3 / 12294	MGIID:	1338890	Length:	1091	Species:	Mus musculus

Alignment Length:	1287	Identity:	396/1287 - (30%)
Similarity:	610/1287 - (47%)	Gaps:	295/1287 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    33 ENINYNLVHSWADKLGMELFHL-----GDFITRRKEVQESFKDAKVVSRNGASIVDSMAKEIEMM 92
            :.|..::|..||...|.|:..:     |..:.::| .:|..||..:...:|..:|..:||.:|.|
Mouse    35 QQIPLSVVKLWASAFGGEIKSIAAKYSGSQLLQKK-YKEYEKDVAIEEIDGLQLVKKLAKIMEEM 98

  Fly    93 MDLKVSAVRRIMDTAENTALSHQNDMADKMFSYYSAKEMLEPGDPVPPIPTPAPDMDKD-----I 152
            ...|..||||:::.||...|.|:.| ||..:.|::|..:.|              .|||     :
Mouse    99 FHKKSEAVRRLVEAAEEAHLKHEFD-ADLQYEYFNAVLINE--------------RDKDGNFLEL 148

  Fly   153 GEPLIYVQPKVVVLEPRPEFHNTPVNFSVSSVHVPVNVFDRAPDVIKAIQWSENLDQIFRDNYKN 217
            |        |..:|.|...|:|.|||.|:|.|.||.|::::.|.::..:.|||:|:::|.||:..
Mouse   149 G--------KEFILAPNDHFNNLPVNISLSDVQVPTNMYNKDPAIVNGVYWSESLNKVFVDNFDR 205

  Fly   218 DPTLSWQFFGSSTGFMRQFPASKWRKDV-PVDLYDCRLRSWYMEAATSPKDIVILMDGSGSMLGQ 281
            ||:|.||:|||:.||.||:|..||..|. .|..:|||.|.||::|||||||:|||:|.||||.|.
Mouse   206 DPSLIWQYFGSAKGFFRQYPGIKWEPDENGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGL 270

  Fly   282 RLDIAKHVVNTILDTLGTNDFVNIFTFDKEVSPVVPCFEDTLIQANLGNIRELKEGIELFRPKSI 346
            ||.|||..|::||||||.:||.||.|:::|:..|.||...||:||:..|....:|.::....|.|
Mouse   271 RLTIAKQTVSSILDTLGDDDFFNIITYNEELHYVEPCLNGTLVQADRTNKEHFREHLDKLFAKGI 335

  Fly   347 ANYTAALTKAFELLEETKLSSRGAQCNQAIMIIGDGAPENNREVFELHNWRDPPYKPVRVFTYLI 411
            .....||.:||.:|.:...:.:|:.|:||||:|.|||.:....:|..:||   |.:.||:|||||
Mouse   336 GMLDIALNEAFNILSDFNHTGQGSICSQAIMLITDGAVDTYDTIFAKYNW---PDRKVRIFTYLI 397

  Fly   412 GKEVANWDDIRWMACENQGYYVHLSDTAEVREMVLNYIPVMARPLVLGRHDHPVIWSQVYADI-- 474
            |:|.|..|:::||||.|:|::..:|..|:|:|.|:.|:.|::||.|:.: :|.|:|::.|.|.
Mouse   398 GREAAFADNLKWMACANKGFFTQISTLADVQENVMEYLHVLSRPKVIDQ-EHDVVWTEAYIDSTL 461

  Fly   475 -EDTKLSDYLWDINQCEYQKADVLEYWQVHDRMLEPSEMHRRKYRRMKETWNQPVDSNVYQFMTT 538
             :..||:|                      |:.|                          ..|||
Mouse   462 PQAQKLAD----------------------DQGL--------------------------VLMTT 478

  Fly   539 VSMPIYDRRENALINLTTDINPAAKINLQVTRIANI-LGVAGTDVPINEIKKLLSPFTLGVNGYA 602
            |:||::.::..                   ||...| |||.|||||:.|:.|.:..:.||::|||
Mouse   479 VAMPVFSKQNE-------------------TRSKGILLGVVGTDVPVKELLKTIPKYKLGIHGYA 524

  Fly   603 FIVTNNGYVLFHPDFRPIFQ--GYILKPAYNSVDMIEVELLDDDRPARDFNPVLMTIRDSIINQS 665
            |.:|||||:|.||:.||:::  ....||.|:|||:.|||..|.|.          .:|::::|:.
Mouse   525 FAITNNGYILTHPELRPLYEEGKKRRKPNYSSVDLSEVEWEDRDD----------VLRNAMVNRK 579

  Fly   666 TGSKWMLVKNHFDEMKRVARVKRQYYWTAIKKTPFTLVISYPEQYGVSHM--DIRADQEIHRISI 728
            ||...|.||...|:.|||..:...||:|.||.|||:|.::....:|....  ::..::.:|.:.
Mouse   580 TGKFSMEVKKTVDKGKRVLVMTNDYYYTDIKGTPFSLGVALSRGHGKYFFRGNVTIEEGLHDLE- 643

  Fly   729 KGTNLRSVFSGKRWKIHPD------WLFCKHSNRTFKTPEIELLYFLERMSEPGWRWPGSRSAMP 787
                            |||      |.:|    .|...||...|..||
Mouse   644 ----------------HPDVSLADEWSYC----NTDLHPEHRHLSQLE----------------- 671

  Fly   788 PEHAAAMFSNNSSTGRYPSINEKESYYCDRQLMQALVFDARVTG-----WFSNNTSFNSKDDKGT 847
               |..::..    |:.|.:.      ||::|:|.::|||.|:.     |.|...:.:...||
Mouse   672 ---AIKLYLK----GKEPLLQ------CDKELIQEVLFDAVVSAPIEAYWTSLALNKSENSDK-- 721

  Fly   848 SASSPIAVLMGLLPRNEFKQRFGVTVAFLATHSGLTRWHEFHSNAAEESGVGETFSQNNTRAIDE 912
                                  ||.||||.|.:||:|.:.|       .|..:..:|:..:|.|:
Mouse   722 ----------------------GVEVAFLGTRTGLSRINLF-------VGAEQLTNQDFLKAGDK 757

  Fly   913 ----------IWYKRAVDQHFVREESFVYSVPFDAGESNSEILVTASHAVFHNEGGKTAPAAVVG 967
                      :||:||.:|   ...|||||:||..|..|...:||||.::...:..|:...|.||
Mouse   758 ENIFNADHFPLWYRRAAEQ---IAGSFVYSIPFSTGTVNKSNVVTASTSIQLLDERKSPVVAAVG 819

  Fly   968 FQFQHSALYKLFHNITGNACAVDDK----------DCYILDNNGYVIISTRVHETGRFFGEVNGA 1022
            .|.:.....:.|...:....::|.|          :||::||||::::|....:||.|||||.||
Mouse   820 IQMKLEFFQRKFWTASRQCASLDGKCSISCDDETVNCYLIDNNGFILVSEDYTQTGDFFGEVEGA 884

  Fly  1023 IMKRLLEENVYRQVTVYDYQAVCFESKNDNNASSMLLSPLFHLLRVGKWLLHTALWYIVQ--LLQ 1085
            :|.:||....::::|:|||||:|..:|..::::..||.|....|...||::...:.::|:  |..
Mouse   885 VMNKLLTMGSFKRITLYDYQAMCRANKESSDSAHGLLDPYKAFLSAAKWIMTELVLFLVEFNLCS 949

  Fly  1086 WAPGVSSHYADMYGDSNDTEPPPPEPHPDHHARNGNGHGKKDDDHWLRYLTLHRTRLK----PCD 1146
            |                                            |...:|....:||    |||
Mouse   950 W--------------------------------------------WHSDMTAKAQKLKQTLEPCD 970

  Fly  1147 MKRDLYTLFNEKDNVVYNMTAHACERPFVVLPIPFSNLILLVIDQLCPRDGSVVLTVNPQPIDYH 1211
            .:...:...........|:....|.:.||:..||.|||.::|:|..|..:....:|:.|..|.|
Mouse   971 TEYPAFVSERTIKETTGNIACEDCSKSFVIQQIPSSNLFMVVVDSSCLCESVAPITMAPIEIRY- 1034

  Fly  1212 LSVNDSLACYKQAREFNRMRPHSCISRHANESGIKLCGKACSVYANLGLLLL 1263
               |:||.|.:...:..|.||.||...|..|:. :.||.|.|:.|...||||
Mouse  1035 ---NESLKCERLKAQKIRRRPESCHGFHPEENA-RECGGASSLQAQAALLLL 1082

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
stj	NP_001246303.1	VWA_N	107..241	CDD:285584	49/138 (36%)
		vWA_VGCC_like	253..439	CDD:238740	86/185 (46%)
		Cache_1	524..619	CDD:280839	33/95 (35%)
Cacna2d3	NP_033915.1	VWA_N	113..229	CDD:285584	49/138 (36%)
		vWA_VGCC_like	242..425	CDD:238740	86/185 (46%)
		MIDAS-like motif	262..266		2/3 (67%)
		Cache_1	452..548	CDD:280839	43/162 (27%)
		VGCC_alpha2	561..633	CDD:285647	26/81 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167832110
Domainoid	1	1.000	163	1.000	Domainoid score	I3947
eggNOG	1	0.900	-	-		E1_KOG2353
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H74929
Inparanoid	1	1.050	577	1.000	Inparanoid score	I1031
Isobase	1	0.950	-	0	Normalized mean entropy	S6129
OMA	1	1.010	-	-		QHG45864
OrthoDB	1	1.010	-	-		D110668at33208
OrthoFinder	1	1.000	-	-		FOG0000496
OrthoInspector	1	1.000	-	-		mtm8846
orthoMCL	1	0.900	-	-		OOG6_103783
Panther	1	1.100	-	-	O	PTHR10166
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5109
SonicParanoid	1	1.000	-	-		X365
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	17	16.750