Protein Alignment ATP8A and DNF2

DIOPT Version :9

• Sequence 1: NP_001137651.1
• Gene: ATP8A / 36488
• FlyBaseID: FBgn0259221
• Length: 1350
• Species: Drosophila melanogaster
• Sequence 2: NP_010378.1
• Gene: DNF2 / 851667
• SGDID: S000002500
• Length: 1612
• Species: Saccharomyces cerevisiae
• Alignment Length: 1597
• Identity: 475/1597 - (29%)
• Similarity: 744/1597 - (46%)
• Gaps: 377/1597 - (23%)

  Fly    66 ESLEHLTPRASDGL------DEQLAASVSHSVH-------SGGS-------------------VT 98
            :.:||.:..||:||      |:........|.|       :|||                   ||
Yeast    13 DDIEHESGSASNGLSSMSPFDDSFQFEKPSSAHGNIEVAKTGGSVLKRQSKPMKDISTPDLSKVT 77

  Fly    99 LCGPPDHSSIH-------LNSANSNL----GFIDSDTPNTPVTPVSGLQPPASVSTSVGG----T 148
            ..|..|:|:.:       ||...:.:    ..:|.|..:...||:          .:.||    .
Yeast    78 FDGIDDYSNDNDINDDDELNGKKTEIHEHENEVDDDLHSFQATPM----------PNTGGFEDVE 132

  Fly   149 LSSRRRSSNRKDSKGSIL-----SRQSGKSRVSLLAQRGLRMTASFLRRKRTEYEDDEDFTSSAG 208
            |.:...|:|...:...:.     :|::...|:.:...:.|:...........|:...||...::.
Yeast   133 LDNNEGSNNDSQADHKLKRVRFGTRRNKSGRIDINRSKTLKWAKKNFHNAIDEFSTKEDSLENSA 197

  Fly   209 YDADDGERRIINLNGPQP-----------TKYGNNRITTAKYSFISFLPAFLFEQFRRYSNCFFL 262
            ......|.|.:..|.|.|           ..|..|:|.|.||:.::|.|..:..||..::|.:||
Yeast   198 LQNRSDELRTVYYNLPLPEDMLDEDGLPLAVYPRNKIRTTKYTPLTFFPKNILFQFHNFANIYFL 262

  Fly   263 LIAILQQIPEVSPTGRYTTLVPLMFILSVSAIKEIIEDIKRHRADNEINH--------------- 312
            ::.||........|......|||:.|:.::|||:.|||.:|...|.|:|:
Yeast   263 ILLILGAFQIFGVTNPGFASVPLIVIVIITAIKDGIEDSRRTVLDLEVNNTRTHILSGVKNENVA 327

  Fly   313 -----------------------------------RSIER-------------------LDS-GS 322
                                               :.:::                   ||| ||
Yeast   328 VDNVSLWRRFKKANTRALIKIFEYFSENLTAAGREKKLQKKREELRRKRNSRSFGPRGSLDSIGS 392

  Fly   323 W------------------------------------------CTVR---WSELTVGDIIKVGIN 342
            :                                          |...   |..:.||||::|..|
Yeast   393 YRMSADFGRPSLDYENLNQTMSQANRYNDGENLVDRTLQPNPECRFAKDYWKNVKVGDIVRVHNN 457

  Fly   343 TFFPADLILLSSSEPQAMCFIETANLDGETNLKIRQALPATAELLETKDLQRLEGRIECELPNRH 407
            ...|||:||||:|:....|::||.|||||||||:||:|..:..:..::|:.|.:..:|.|.|:.:
Yeast   458 DEIPADMILLSTSDVDGACYVETKNLDGETNLKVRQSLKCSKIIKSSRDITRTKFWVESEGPHAN 522

  Fly   408 LYEF----------NGVLRETGKPPAALGNDQVLQRGAILRNTAWVFGIVVYSGQETKLMKNSTS 462
            ||.:          ||.:|   ..|..:.|  :|.||..||||.|..|:|:::|.:||:|.|:..
Yeast   523 LYSYQGNFKWQDTQNGNIR---NEPVNINN--LLLRGCTLRNTKWAMGMVIFTGDDTKIMINAGV 582

  Fly   463 APLKRSTVDKLTNTQILMLFMILISLCIISGLCN-LFWTREHSETDWY-LGLTDFKTKSLGYNLL 525
            .|.|:|.:.:..|..:::.|::|..||..:|:.| :::.::....|:: .|.......:.|:  :
Yeast   583 TPTKKSRISRELNFSVILNFVLLFILCFTAGIVNGVYYKQKPRSRDYFEFGTIGGSASTNGF--V 645

  Fly   526 TFF---ILYNNLIPISLQVTLELVRFLQAIFINYDIEMYHEESNTPAMARTSNLNEELGMVKYIF 587
            :|:   |||.:|:||||.:::|:::..|||||..|:.:|:.:.:.|...::.|::::||.::|||
Yeast   646 SFWVAVILYQSLVPISLYISVEIIKTAQAIFIYTDVLLYNAKLDYPCTPKSWNISDDLGQIEYIF 710

  Fly   588 SDKTGTLTQNVMEFKKCSIAGYVYTAERT------------------------------------ 616
            |||||||||||||||||:|.|..|....|
Yeast   711 SDKTGTLTQNVMEFKKCTINGVSYGRAYTEALAGLRKRQGVDVESEGRREKEEIAKDRETMIDEL 775

  Fly   617 ----------PEE-----SQLVQNILGR---HETSAVIEEFLELLSVCHTVI--PERKENGNMIY 661
                      ||:     .::|:::.|.   |:... .|.||..|::||:|:  |.:.:...:..
Yeast   776 RSMSDNTQFCPEDLTFVSKEIVEDLKGSSGDHQQKC-CEHFLLALALCHSVLVEPNKDDPKKLDI 839

  Fly   662 HAASPDERALVEGAQKFGYIF--DTRTPKYVEINALGVRKRYEVLNVLEFTSSRKRMSLIVRTP- 723
            .|.||||.|||..|::.||.|  .:::...|||.  ||:|.::|||||||.|||||||.|::.|
Yeast   840 KAQSPDESALVSTARQLGYSFVGSSKSGLIVEIQ--GVQKEFQVLNVLEFNSSRKRMSCIIKIPG 902

  Fly   724 -----ENKIKLFCKGADTVIYERL-APQGQA-FREQTLRHLEEFASDGLRTLCLAVADIRPDVYQ 781
                 |.|..|.|||||:|||.|| ..|..| ..|:|..||||:|::||||||||..::....|:
Yeast   903 STPKDEPKALLICKGADSVIYSRLDRTQNDATLLEKTALHLEEYATEGLRTLCLAQRELTWSEYE 967

  Fly   782 EWSQTFDKASVALQNRESKLEDAANLIENNLRLLGATAIEDRLQDGVPETIASLLDAGIYIWVLT 846
            .|.:|:|.|:.::.|||.:|:...::||..|.|||.||||||||||||::||.|.:|||.:||||
Yeast   968 RWVKTYDVAAASVTNREEELDKVTDVIERELILLGGTAIEDRLQDGVPDSIALLAEAGIKLWVLT 1032

  Fly   847 GDKQETAINIGYSCRLISHSMDIIILNEESLDA--------------TREVIHRHYG------EF 891
            |||.|||||||:||.::::.|:::::.....|.              ..:.:...:|      |.
Yeast  1033 GDKVETAINIGFSCNVLNNDMELLVVKASGEDVEEFGSDPIQVVNNLVTKYLREKFGMSGSEEEL 1097

  Fly   892 KSSSAK----DANVALVIDGTTLKYALSC-DLRNDFQDLCILCRVVICCRVSPMQKAEVVEMVTQ 951
            |.:..:    ..|.|::|||..||.||:. ::|..|..||..|:.|:||||||.|||.||::|.:
Yeast  1098 KEAKREHGLPQGNFAVIIDGDALKVALNGEEMRRKFLLLCKNCKAVLCCRVSPAQKAAVVKLVKK 1162

  Fly   952 STNAVTLAIGDGANDVAMIQKANVGIGISGVEGLQAACASDYSIAQFRYLQRLLLVHGAWNYARI 1016
            :.:.:|||||||:|||||||.|:||:||:|.||.||...|||:|.||||:.||:||||.|.|.|:
Yeast  1163 TLDVMTLAIGDGSNDVAMIQSADVGVGIAGEEGRQAVMCSDYAIGQFRYVTRLVLVHGKWCYKRL 1227

  Fly  1017 SKLILYSFYKNVCLYVIELWFAVYSGWSGQILFERWTIGLYNVLFTAMPPFAMGLFEKFCTAETM 1081
            :::|...|||||...:...|:.:|:.:.|..|||...:..||:.||::|...:.:.::..:....
Yeast  1228 AEMIPQFFYKNVIFTLSLFWYGIYNNFDGSYLFEYTYLTFYNLAFTSVPVILLAVLDQDVSDTVS 1292

  Fly  1082 IRYPMLYKTSQNAKLFNVKVFWIWIFNALLHSVFLFWLPLAAYTKE-VIWSDGKTSDYLLMGNLV 1145
            :..|.||:.....|.:|...|..::.:.:..||..|:.|..||.|. |:..:|...|:.....:.
Yeast  1293 MLVPQLYRVGILRKEWNQTKFLWYMLDGVYQSVICFFFPYLAYHKNMVVTENGLGLDHRYFVGVF 1357

  Fly  1146 YTYVIVTVC-LKAGLITNSWTWLTHLAIWGSIVLWFGFLLIYSHVWPMFKFASNFRGMDIQLLST 1209
            .|.:.||.| ....:....|.|...|.|..|:.:::|    ::.:|.....::.|.....::.:.
Yeast  1358 VTAIAVTSCNFYVFMEQYRWDWFCGLFICLSLAVFYG----WTGIWTSSSSSNEFYKGAARVFAQ 1418

  Fly  1210 PVFY--------FCLMLVPITTLLIDVICKLVHNTVFKTLTEAVRE----------------TEI 1250
            |.::        |||:  |..|  ||.|.|:.:.   |.: |.|||                |:.
Yeast  1419 PAYWAVLFVGVLFCLL--PRFT--IDCIRKIFYP---KDI-EIVREMWLRGDFDLYPQGYDPTDP 1475

  Fly  1251 RRSDISEV-----MNEPRSSDSGFGYALGKLIRSSHILISHDHHPVSESSRTSNLRVENLEMEAD 1310
            .|..|:|:     ..||.|.|:.|                   ..||.|..|  :..|.:.|..
Yeast  1476 SRPRINEIRPLTDFKEPISLDTHF-------------------DGVSHSQET--IVTEEIPMSI- 1518

  Fly  1311 TEQQQQSRQRQKWRGGQVATGVARSARNSTTSPITAT 1347
            ...:|.||     :|.:|:|.:   .|....||:|.|
Yeast  1519 LNGEQGSR-----KGYRVSTTL---ERRDQLSPVTTT 1547

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ATP8A	NP_001137651.1	PhoLip_ATPase_N	218..280	CDD:292826	19/72 (26%)
		ATPase-Plipid	229..1247	CDD:273734	408/1248 (33%)
		E1-E2_ATPase	286..>371	CDD:278548	38/199 (19%)
		Cation_ATPase	645..741	CDD:289987	49/105 (47%)
		HAD_like	<942..1001	CDD:304363	36/58 (62%)
		PhoLip_ATPase_C	988..1240	CDD:292829	72/261 (28%)
DNF2	NP_010378.1	ATPase-Plipid	229..1456	CDD:273734	408/1248 (33%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0206
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000076
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100089
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.710