DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mdr49 and pgp-2

DIOPT Version :9

Sequence 1:	NP_523724.2	Gene:	Mdr49 / 36428	FlyBaseID:	FBgn0004512	Length:	1302	Species:	Drosophila melanogaster
Sequence 2:	NP_001300410.1	Gene:	pgp-2 / 172262	WormBaseID:	WBGene00003996	Length:	1296	Species:	Caenorhabditis elegans

Alignment Length:	1316	Identity:	494/1316 - (37%)
Similarity:	743/1316 - (56%)	Gaps:	87/1316 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 EGSVVDATRKYSYFDLFRYSTRCERFLLVVSLLVATAASAFIPYFMIIYGEFTSLLV-----DRT 78
            :.|:.::|.|.:.:.:|.|:...:..||:...:.|....|..|...|:.|..|::.:     |..
Worm    30 DSSIDESTVKLTNYGIFYYTQGVDLLLLITGTVAAVIHGAGFPLLAIVLGGMTTVFLRAQNSDFV 94

  Fly    79 VGVGTSSPAFALPMFGGGQQLTNASKEENNQAIIDDATAFGIGSLVGSVAMFLLITLAIDLANRI 143
            |||...:|...:|:          |.:|.|..::    .:.|..||..|.||....:.|......
Worm    95 VGVDNVNPEGLVPI----------SLDEFNSEVV----KYCIYYLVLGVLMFFTSYVQIACFESY 145

  Fly   144 ALNQIDRIRKLFLEAMLRQDIAWYDTSSGSNFASKMTEDLDKLKEGIGEKIVIVVFLIMTFVIGI 208
            |...:.::|:.:|:|:|||.|.|:|.....|..:::|:||::::||:|:|..::|.:...|:.|.
Worm   146 AERLVHKLRQNYLKAILRQQIQWFDKQQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGY 210

  Fly   209 VSAFVYGWKLTLVVLSCVPFIIAATSVVARLQGSLAEKELKSYSDAANVVEEVFSGIRTVFAFSG 273
            ...|.|.|.:|||::...|.|:.:.:.:::...:....|.::|:.|..:.||.||.||||.:.:|
Worm   211 GVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNG 275

  Fly   274 QEKEKERFGKLLIPAENTGRKKGLYSGMGNALSWLIIYLCMALAIWYGVTLILDERDLPDRVYTP 338
            .::|.:||...|.....||..|..|.|:|...|.|.:|...|||.|||.|||::     |..:..
Worm   276 HKRELDRFYNALEVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIIN-----DPTFDR 335

  Fly   339 AVLVIVLFAVIMGAQNLGFASPHVEAIAVATAAGQTLFNIIDRPSQVDPMDEKGNRPENTAGHIR 403
            .::..|.|||:.|:.:||.|.||:.:...|..|..|:..:|:...::||...:|...:|..|.|.
Worm   336 GLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVDNMKGDIS 400

  Fly   404 FEGIRFRYPARPDVEILKGLTVDVLPGQTVAFVGASGCGKSTLIQLMQRFYDPEAGSVKLDGRDL 468
            |:.:.||||:|.|:.:|||:::::..|..:|.||:|||||||::.|:||||||..|.|.:||.||
Worm   401 FKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDL 465

  Fly   469 RTLNVGWLRSQIGVVGQEPVLFATTIGENIRYGRPSATQADIEKAARAANCHDFITRLPKGYDTQ 533
            |.:||..||.|||:|.||||||..||.|||:.|...||...:.:|.:.||.:|||.|||.||.|:
Worm   466 REVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHATHDQVVEACKMANANDFIKRLPDGYGTR 530

  Fly   534 VGEKGAQISGGQKQRIAIARALVRQPQVLLLDEATSALDPTSEKRVQSALELASQGPTTLVVAHR 598
            |||||.|:|||||||||||||||:.|::|||||||||||..:|:.||.||:.|..|.||::||||
Worm   531 VGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQAQAGRTTIIVAHR 595

  Fly   599 LSTITNADKIVFLKDGVVAEQGTHEELMERRGLYCELVSITQRKEATEADEGAVAGRPLQKSQNL 663
            ||||.|.|:|...|.|.:.|.|:|||||.::|::.::         |:|          |..:..
Worm   596 LSTIRNVDRIFVFKAGNIVESGSHEELMSKQGIFYDM---------TQA----------QVVRQQ 641

  Fly   664 SDEETDDDEEDEEEDEEPELQTSGSSRDSGFRASTRRKRRSQRRK------KKKDKEVV-SKV-- 719
            ..|...|.|:...|.....|....|:| |....:|...:.::..:      .:.:::|: |::
Worm   642 QQEAGKDIEDTISESAHSHLSRKSSTR-SAISIATSIHQLAEEVELIDPFNGQTNQDVIRSRILS 705

  Fly   720 -----------SFTQLMKLNSPEWRFIVVGGIASVMHGATFPLWGLFFGDFFGILSDGDDDVVRA 773
                       |..::.|.|..:..:.:.|...:.:.|:..|::.|.:.:.|.:.|...|. ::|
Worm   706 AFGGECKAPPTSMFKIFKFNGDKVGWFIGGIFGAFIFGSVTPVFALVYAEIFNVYSLPADQ-MQA 769

  Fly   774 EVLKISMIFVGIGLMAGLGNMLQTYMFTTA------GVKMTTRLRKRAFGTIIGQDIAYFDDERN 832
            .|.....:||.:|:...:|      .||:|      |..:|.:||..||..::.||||::||.|:
Worm   770 NVYFWCGMFVLMGITFFVG------FFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRH 828

  Fly   833 SVGALCSRLASDCSNVQGATGARVGTMLQAVATLVVGMVVGFVFSWQQTLLTLVTLPLVCLSVYL 897
            ..|.||:|.|:|..||: ....|:..:|.::.|:...:.:||.:.||..|:.:|.:||:.:..|.
Worm   829 GTGKLCTRFATDAPNVR-YVFTRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYF 892

  Fly   898 EGRFIMKSAQKAKASIEEASQVAVEAITNIRTVNGLCLERQVLDQYVQQIDRVDIACRRKVRFRG 962
            |.:.......:....:|||.:||.:|:.:||||:.|..:.|....|.:.:........:.....|
Worm   893 EMQMRFGKQIRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFTYCEYLREPFNTNLKHAHTYG 957

  Fly   963 LVFALGQAAPFLAYGISMYYGGILVAEERMNYEDIIKVAEALIFGSWMLGQALAYAPNVNDAILS 1027
            .|||..|:..|..|..:.|.|.|.|.::.|...|:.:|..|:.|...|:|...::.|:|..|.|:
Worm   958 AVFAFSQSLIFFMYAAAFYLGSIFVNQQAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLA 1022

  Fly  1028 AGRLMDLFKRTSTQPNPPQSPYNT--VEKSEGDIVYENVGFEYPTRKGTPILQGLNLTIKKSTTV 1090
            |..|..|.:    .|.|..|..::  |:...|:|...||.|.|||||.|.:|||..|.||...||
Worm  1023 ASLLFYLIE----HPTPIDSLSDSGIVKPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTV 1083

  Fly  1091 ALVGPSGSGKSTCVQLLLRYYDPVSGSVNLSGVPSTEFPLDTLRSKLGLVSQEPVLFDRTIAENI 1155
            ||||.||.||||.:.||.|:|:...|.:.:.|.......:.:||.::.:|||||.|||.||.|||
Worm  1084 ALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENI 1148

  Fly  1156 AYGNNFRDDVSMQEIIEAAKKSNIHNFISALPQGYDTRLG-KTSQLSGGQKQRIAIARALVRNPK 1219
            .||.|  .:|:.|||:||||.:||||||..||.||||.:| |.:||||||||||||||||||:|.
Worm  1149 CYGTN--RNVTYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPS 1211

  Fly  1220 ILILDEATSALDLESEKVVQQALDEARSGRTCLTIAHRLTTVRNADLICVLKRGVVVEHGTHDEL 1284
            :|:|||||||||.||||:||:|||.|:.|||||.|||||:|::|:|:|.::..|.:||.||||||
Worm  1212 VLLLDEATSALDTESEKIVQEALDAAKQGRTCLVIAHRLSTIQNSDVIAIVSEGKIVEKGTHDEL 1276

  Fly  1285 MALNKIYANLYLMQQV 1300
            :..::||......|::
Worm  1277 IRKSEIYQKFCETQRI 1292

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mdr49	NP_523724.2	MdlB	34..640	CDD:224055	242/610 (40%)
		ABC_membrane	116..352	CDD:279056	75/235 (32%)
		ABC_MTABC3_MDL1_MDL2	402..636	CDD:213216	133/233 (57%)
		MdlB	735..1299	CDD:224055	231/572 (40%)
		ABC_membrane	739..998	CDD:279056	75/264 (28%)
		ABC_MTABC3_MDL1_MDL2	1059..1298	CDD:213216	138/239 (58%)
pgp-2	NP_001300410.1	PTZ00265	56..1286	CDD:240339	488/1282 (38%)
		ABC_membrane	61..349	CDD:279056	90/306 (29%)
		ABC_MTABC3_MDL1_MDL2	399..632	CDD:213216	133/232 (57%)
		ABC_membrane	736..999	CDD:279056	76/270 (28%)
		ABC_MTABC3_MDL1_MDL2	1052..1290	CDD:213216	138/239 (58%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	151	1.000	Domainoid score	I2660
eggNOG	1	0.900	-	-		E2759_KOG0055
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H69084
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S2081
OMA	1	1.010	-	-		QHG63700
OrthoDB	1	1.010	-	-		D186078at2759
OrthoFinder	1	1.000	-	-		FOG0000106
OrthoInspector	1	1.000	-	-		mtm4744
orthoMCL	1	0.900	-	-		OOG6_100059
Panther	1	1.100	-	-	O	PTHR24221
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R932
SonicParanoid	1	1.000	-	-		X92
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.770