DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mdr49 and abcb4

DIOPT Version :9

Sequence 1:	NP_523724.2	Gene:	Mdr49 / 36428	FlyBaseID:	FBgn0004512	Length:	1302	Species:	Drosophila melanogaster
Sequence 2:	NP_001303643.1	Gene:	abcb4 / 100136865	ZFINID:	ZDB-GENE-080204-52	Length:	1275	Species:	Danio rerio

Alignment Length:	1318	Identity:	554/1318 - (42%)
Similarity:	809/1318 - (61%)	Gaps:	80/1318 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 KKEESRLPQAGDFQLKEGSVVDATRKYSY-----FDLFRYSTRCERFLLVVSLLVATAASAFIPY 62
            ||||:     ||...::....:...|...     .:||||:...:..|:::.|:::.|..|.:|.
Zfish    13 KKEEN-----GDVSGEKNGKEEKEEKEKLEMVGPIELFRYADSIDILLMMLGLIMSMANGAVLPL 72

  Fly    63 FMIIYGEFTSLLVDRTVGVG----TSSPAFALPMFGG---GQQLTNASKEENNQAIIDDATAFGI 120
            .:|::|:.|...||.|:...    |..|.|..|....   |:::|.            .|..:.|
Zfish    73 MVIVFGDMTDSFVDDTLLDNLKNITLPPNFTFPETSNITLGEKMTT------------HAIYYSI 125

  Fly   121 GSLVGSVAMFLLI---TLAIDLANRIALNQIDRIRKLFLEAMLRQDIAWYDTSSGSNFASKMTED 182
            ...|..||.::.:   |||       |..|:.::||:|..::::|:|.|:|.:......:::|:|
Zfish   126 MGFVVLVAAYMQVAFWTLA-------AGRQVKKLRKIFFHSIMKQEIGWFDVNETGQLNTRLTDD 183

  Fly   183 LDKLKEGIGEKIVIVVFLIMTFVIGIVSAFVYGWKLTLVVLSCVPFIIAATSVVARLQGSLAEKE 247
            :.|:.||||:|:.:::..:.||::||:..|..|||||||:|:..|.:..:.:|:.::..:...||
Zfish   184 VYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKE 248

  Fly   248 LKSYSDAANVVEEVFSGIRTVFAFSGQEKEKERFGKLLIPAENTGRKKGLYSGMGNALSWLIIYL 312
            ..:|:.|..|.|||.|.|||||||.||:||.:|:.|.|..|:|.|.:|.:...:....::.:||:
Zfish   249 QTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYM 313

  Fly   313 CMALAIWYGVTLILDERDLPDRVYTPAVLVIVLFAVIMGAQNLGFASPHVEAIAVATAAGQTLFN 377
            ..|||.|||.||||...      ||..:|:.:.|||::||..||..||:::..:.|..|...:|.
Zfish   314 SYALAFWYGSTLILGGE------YTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQ 372

  Fly   378 IIDRPSQVDPMDEKGNRPENTAGHIRFEGIRFRYPARPDVEILKGLTVDVLPGQTVAFVGASGCG 442
            |||...:::...|:|.:.:...|:|.|:.|.||||:|.||::|.|:.:.|:.|||:|.||:||||
Zfish   373 IIDHEPKINSFSEEGYKLDVVKGNIEFKNIHFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCG 437

  Fly   443 KSTLIQLMQRFYDPEAGSVKLDGRDLRTLNVGWLRSQIGVVGQEPVLFATTIGENIRYGRPSATQ 507
            |||.|||:||||||:.|||.:||.|:|:|||..||..||||.||||||||||.|||||||...||
Zfish   438 KSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYGRQDVTQ 502

  Fly   508 ADIEKAARAANCHDFITRLPKGYDTQVGEKGAQISGGQKQRIAIARALVRQPQVLLLDEATSALD 572
            .:||:|||.||.::||.:||..::|.||::|.|:||||||||||||||||.|::|||||||||||
Zfish   503 DEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALD 567

  Fly   573 PTSEKRVQSALELASQGPTTLVVAHRLSTITNADKIVFLKDGVVAEQGTHEELMERRGLYCELVS 637
            ..||..||:||:....|.||:|||||||||.|||.|...::|.:.|.|||:|||||:|:|..||:
Zfish   568 AESETIVQAALDKVRLGRTTIVVAHRLSTIRNADVIAGFQNGEIVELGTHDELMERKGIYHSLVN 632

  Fly   638 ITQRKEATEADEGAVAGRPLQKSQNLSDEETDDDEEDEEEDEEPELQTSGSSRDSGFRASTRRKR 702
            :...|                        .|:..|||.||....|...|.||.:.  |...|:|.
Zfish   633 MQMFK------------------------STEVAEEDSEEMTMDEKSPSVSSMNE--RTLFRQKS 671

  Fly   703 RSQRRKKKK------DKEVVSKVSFTQLMKLNSPEWRFIVVGGIASVMHGATFPLWGLFFGDFFG 761
            ||...|:.|      ::|.|..|||..::|||.|||.::|||.:.:.::|...|.:.:.|.....
Zfish   672 RSGSEKELKEEEKPTEEEKVPNVSFLTVLKLNYPEWPYMVVGILCATINGGMQPAFAVIFSKIIA 736

  Fly   762 ILSDGDDDVVRAEVLKISMIFVGIGLMAGLGNMLQTYMFTTAGVKMTTRLRKRAFGTIIGQDIAY 826
            :.::.|.::||......|::|.|||:::.....||.:.|..||..:|.|||.:||..::.||:|:
Zfish   737 VFAEPDQNLVRQRCDLYSLLFAGIGVLSFFTLFLQGFCFGKAGELLTMRLRFKAFNAMMRQDLAW 801

  Fly   827 FDDERNSVGALCSRLASDCSNVQGATGARVGTMLQAVATLVVGMVVGFVFSWQQTLLTLVTLPLV 891
            :||.:||||||.:|||:|.:.||||||.|:.|:.|.||.|...:|:.||:.||.|||.|..:|::
Zfish   802 YDDTKNSVGALTTRLAADTAQVQGATGVRLATLAQNVANLGTAIVISFVYGWQLTLLILSIVPIM 866

  Fly   892 CLSVYLEGRFIMKSAQKAKASIEEASQVAVEAITNIRTVNGLCLERQVLDQYVQQIDRVDIACRR 956
            .::..::.:.:...|.|.|..:|:|.::|.|||.|:|||..|..|.:....|.:.:.......::
Zfish   867 AVAGAIQMKLLAGHALKDKKELEQAGKIATEAIENVRTVVSLTRESKFESLYEENLIVPYKNAKK 931

  Fly   957 KVRFRGLVFALGQAAPFLAYGISMYYGGILVAEERMNYEDIIKVAEALIFGSWMLGQALAYAPNV 1021
            |....||.|:..||..:.||.....:|..|:.::.|.:|.:..|..|:::|:..:|:|.::.||.
Zfish   932 KAHVFGLTFSFSQAMIYFAYAGCFKFGSWLIEQKLMTFEGVFLVISAVVYGAMAVGEANSFTPNY 996

  Fly  1022 NDAILSAGRLMDLFKRTSTQPNPPQSPYNTVEKSEGDIVYENVGFEYPTRKGTPILQGLNLTIKK 1086
            ..|.:||..::.|..|.....|..:.. :..:|.||::.:|:|.|:||:|...|:||||.|.:||
Zfish   997 AKAKMSASHVLMLINRAPAIDNSSEDG-DKPDKFEGNVGFEHVYFKYPSRPDVPVLQGLKLRVKK 1060

  Fly  1087 STTVALVGPSGSGKSTCVQLLLRYYDPVSGSVNLSGVPSTEFPLDTLRSKLGLVSQEPVLFDRTI 1151
            ..|:||||.||.||||.:|||.|:|||..|.|.|....:.:..:..|||::|:|||||||||.::
Zfish  1061 GQTLALVGSSGCGKSTTIQLLERFYDPQQGRVMLDDNDAKQLNIHWLRSQIGIVSQEPVLFDCSL 1125

  Fly  1152 AENIAYGNNFRDDVSMQEIIEAAKKSNIHNFISALPQGYDTRLG-KTSQLSGGQKQRIAIARALV 1215
            |||||||:|.| :|..:||:||||.:|||:||..|||.|.|:.| |.:|||||||||||||||::
Zfish  1126 AENIAYGDNSR-EVDQEEIVEAAKAANIHSFIENLPQRYQTQAGDKGTQLSGGQKQRIAIARAIL 1189

  Fly  1216 RNPKILILDEATSALDLESEKVVQQALDEARSGRTCLTIAHRLTTVRNADLICVLKRGVVVEHGT 1280
            ||||:|:|||||||||.||||:||.|||:|..||||:.:||||:|::|||.|.|::.|||||.||
Zfish  1190 RNPKVLLLDEATSALDTESEKIVQDALDKASKGRTCIIVAHRLSTIQNADCIAVVQNGVVVEQGT 1254

  Fly  1281 HDELMALNKIYANLYLMQ 1298
            |.:|::....|..|...|
Zfish  1255 HQQLLSQQGAYYTLVTSQ 1272

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mdr49	NP_523724.2	MdlB	34..640	CDD:224055	271/615 (44%)
		ABC_membrane	116..352	CDD:279056	84/238 (35%)
		ABC_MTABC3_MDL1_MDL2	402..636	CDD:213216	148/233 (64%)
		MdlB	735..1299	CDD:224055	247/565 (44%)
		ABC_membrane	739..998	CDD:279056	87/258 (34%)
		ABC_MTABC3_MDL1_MDL2	1059..1298	CDD:213216	140/239 (59%)
abcb4	NP_001303643.1	PTZ00265	55..1266	CDD:240339	540/1263 (43%)
		ABC_membrane	60..348	CDD:279056	102/312 (33%)
		ABC_MTABC3_MDL1_MDL2	397..634	CDD:213216	150/236 (64%)
		ABC_membrane	714..984	CDD:279056	90/269 (33%)
		ABC_MTABC3_MDL1_MDL2	1035..1272	CDD:213216	140/237 (59%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	210	1.000	Domainoid score	I2768
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG63700
OrthoDB	1	1.010	-	-		D186078at2759
OrthoFinder	1	1.000	-	-		FOG0000106
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100059
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R932
SonicParanoid	1	1.000	-	-		X92
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	10	9.860