DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Amph and Amph

DIOPT Version :9

Sequence 1:	NP_523717.1	Gene:	Amph / 36383	FlyBaseID:	FBgn0027356	Length:	602	Species:	Drosophila melanogaster
Sequence 2:	NP_071553.1	Gene:	Amph / 60668	RGDID:	620274	Length:	683	Species:	Rattus norvegicus

Alignment Length:	747	Identity:	199/747 - (26%)
Similarity:	313/747 - (41%)	Gaps:	214/747 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MTENKGIMLAKSVQKHAGRAKEKILQNLGKVDRTADEIFDDHLNNFNRQQASANRLQKEFNNYIR 65
            |.:.|..:.||:|||...||:||:||.|||.|.|.||.|::::.||.||:|...|||:|...|:.
  Rat     1 MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLA 65

  Fly    66 CVRAAQAASKTLMDSVCEIYEPQWSGYDALQAQTGASESLWADFAHKLGDQVLIPLNTYTGQFPE 130
            .::..|.||..|.:|:.|:|||.|.|.:.::......:.||.||..||.|..|:.|:||.||||:
  Rat    66 AIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPD 130

  Fly   131 MKKKVEKRNRKLIDYDGQRHSFQNLQANANKRKDDVKLTKGREQLEEARRTYEILNTELHDELPA 195
            :|.::.||:|||:|||..||..:.||  ::||||:.:::|..|:.::|::.:|..|.:|.:|||:
  Rat   131 IKNRIAKRSRKLVDYDSARHHLEALQ--SSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPS 193

  Fly   196 LYDSRILFLVTNLQTLFATEQVFHNETAKIYSELEAIVDKLATE------------SQRG----- 243
            |:..|:.|.|...:.:.:.|..||.|.|.:..:|..::.||..:            |..|
  Rat   194 LWSRRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIA 258

  Fly   244 -------------------SNTLRKQTSNPIKTSSPVQ-------------SPVNKLNNANI--- 273
                               ::||...:..|::..||.|             :|..:|...||
  Rat   259 KTPSPPEEASPLPSPTASPNHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELQQENIINF 323

  Fly   274 -NSNY----------QNQ---------------------ITTNGGSSLANSPTS---------TS 297
             ..|:          ||:                     :|..|.::..:||.|         ||
  Rat   324 FEDNFVPEINVTTPSQNEVLEVKKEETLLDLDFDPFKPDVTPAGSAAATHSPMSQTLPWDLWTTS 388

  Fly   298 SSLQEPR-------FDSVSST-------------------PEPRPESPAAALVSATPSSPVENGV 336
            :.|.:|.       |.....|                   .||:.|.|..  .:|.|::.::.|:
  Rat   389 TDLVQPASGGSFNDFTQPQDTSLFTMQTDQNMAETEQALPTEPQAEEPPT--TAAAPTAGLDLGL 451

  Fly   337 TTKSLERPELSGL---NASAKATTTTQTSPTEDKAVVSEAVKPSETEGAAVAASVTPAPPATPAQ 398
               .:|.|:....   ...|..|..|:.|..|:          :|.|.||:.|....:|......
  Rat   452 ---EMEEPKEEAAIPPGTDAGETVGTEGSTGEE----------AEAEKAALPAGEGESPEGAKID 503

  Fly   399 I------NGNNNEPSIVKEGGKQPKELPSTTSNAEAAAEAAANNGNSIEEHKQKKLGNDTTVTAT 457
            :      :..:.:.:.::.|..|.|.:||      ...|.|:|:..  |||::       |.|.|
  Rat   504 VESTELASSESPQAAELEAGAPQEKVIPS------VVIEPASNHEG--EEHQE-------TTTGT 553

  Fly   458 ETVTVTQHSVTSTDTDNIVTISDTNTDTDTKTSTGTSQK--GRPVPVVNRHSVNNLNKNPFEDDD 520
            ||...|:                   |...:...|..|:  ..|.|:                |.
  Rat   554 ETREATE-------------------DVAPQGPAGEKQELATEPTPL----------------DS 583

  Fly   521 ERIYEVPADANTA---------DLPPGVLYRVKATYGYAKEDVDELSFEIGDLIRVIEYDDPEDQ 576
            :.....||.|..|         :||||.||:|:..:.:...:.|||:.:.||::.|:..|...||
  Rat   584 QAATPAPAGAVDASLSAGDAAQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQ 648

  Fly   577 EEGWLMGQKEG--------TNEKGLFPANFTR 600
            :.|||:|.||.        ...|||||.||||
  Rat   649 DAGWLVGVKESDWLQYRDLATYKGLFPENFTR 680

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Amph	NP_523717.1	BAR_Amphiphysin	26..238	CDD:153272	83/211 (39%)
Amph	NP_523717.1	SH3_Amphiphysin	539..602	CDD:212724	27/70 (39%)
Amph	NP_071553.1	BAR_Amphiphysin_I_II	26..236	CDD:153295	81/209 (39%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	244..312		24/150 (16%)
		DUF4676	258..>316	CDD:292353	23/140 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	421..443		5/21 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	455..599		43/162 (27%)
		SH3_Amphiphysin_I	611..682	CDD:213016

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166338304
Domainoid	1	1.000	186	1.000	Domainoid score	I3255
eggNOG	1	0.900	-	-		E1_KOG3771
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	250	1.000	Inparanoid score	I3146
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1366218at2759
OrthoFinder	1	1.000	-	-		FOG0001177
OrthoInspector	1	1.000	-	-		otm44397
orthoMCL	1	0.900	-	-		OOG6_105748
Panther	1	1.100	-	-	O	PTHR46514
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1098
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	14	13.760