DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ClC-b and CLCN4

DIOPT Version :9

Sequence 1:	NP_610798.1	Gene:	ClC-b / 36381	FlyBaseID:	FBgn0033755	Length:	813	Species:	Drosophila melanogaster
Sequence 2:	NP_001821.2	Gene:	CLCN4 / 1183	HGNCID:	2022	Length:	760	Species:	Homo sapiens

Alignment Length:	834	Identity:	230/834 - (27%)
Similarity:	375/834 - (44%)	Gaps:	196/834 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    82 INGSAGSPNRNINS--SEPIFHLRSRTAAASTPNYESLDYEVCENTLFQDEQRKRLTER-----F 139
            :|..|.|.:.|:..  .||.      ....:..::.::|: :.|.:...|..|| :|.:     :
Human     2 VNAGAMSGSGNLMDFLDEPF------PDVGTYEDFHTIDW-LREKSRDTDRHRK-ITSKSKESIW 58

  Fly   140 SLRKDII----RWIIFIQIGIITALIACTIDIIIEELSIRKYTFLYNSVKENVPLS--------- 191
            ...|.::    .|::.:.||::...:|..||:.::.::         .:||.|.||
Human    59 EFIKSLLDAWSGWVVMLLIGLLAGTLAGVIDLAVDWMT---------DLKEGVCLSAFWYSHEQC 114

  Fly   192 -------DASDRD---------------------LLIPYLYWLLFSIVPVAFGAAMVTYIEPITA 228
                   ...|||                     .::.||.::|::::......::|....|...
Human   115 CWTSNETTFEDRDKCPLWQKWSELLVNQSEGASAYILNYLMYILWALLFAFLAVSLVRVFAPYAC 179

  Fly   229 GSGIPQVKSYLNGVKIPRIVRIKTLAVKAIGVITSVVGGLAGGKEGPMIHAGAVVAAGISQGKST 293
            |||||::|:.|:|..|...:...||.:|.:.::..|..||:.|||||::|    ||.......|:
Human   180 GSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVLVVSSGLSLGKEGPLVH----VACCCGNFFSS 240

  Fly   294 TFLKDFRIFKAFRDDHEKRDFVLGGGAAGVSAAFGAPIGGMLFSLEEAASFWNQNLIWRTLVASI 358
            .|      .|..:::.::|:.:....|||||.||||||||:||||||.:.::....:||:..|::
Human   241 LF------SKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAAL 299

  Fly   359 ISVFTLNIVLSAYHGLNDF--TFTGLFNLGKFDTPLKFDYFELPIFMILGVTGGLLGAAWNSLNT 421
            ::.|||       ..:|.|  :...||.: ::.||  :...||..|::|||.|||    |.:|..
Human   300 VAAFTL-------RSINPFGNSRLVLFYV-EYHTP--WYMAELFPFILLGVFGGL----WGTLFI 350

  Fly   422 KINKFRKRFIPW-------KIGK--VLEAVVVAMMGVTLAC-----------LMIYFINDCRPLG 466
            :.|      |.|       ::||  |||.:||..:...:|.           |:....|||..|.
Human   351 RCN------IAWCRRRKTTRLGKYPVLEVIVVTAITAIIAYPNPYTRQSTSELISELFNDCGALE 409

  Fly   467 --------NDPT-NNPVQLFCEDNEYNAVAALWFQTPEATVRSLFHDPPGSHKILT----LALFT 518
                    |||. ..||    :|                     ..|.|....:.|    |||..
Human   410 SSQLCDYINDPNMTRPV----DD---------------------IPDRPAGVGVYTAMWQLALAL 449

  Fly   519 VVYYVLSCATFGLNVSLGVFIPTALVGAAWGRLLAM----LTYY-----VF-----PQAEFLHPG 569
            :...|::..|||:.:..|:|||:..|||..||::.:    |.|:     :|     |.|:.:.||
Human   450 IFKIVVTIFTFGMKIPSGLFIPSMAVGAIAGRMVGIGVEQLAYHHHDWIIFRNWCRPGADCVTPG 514

  Fly   570 KYALIGAAANLGGVLRMTISLSVILMETTGVETSFFFPLIIALISAKWVGDYF-NEGIYDTQIQV 633
            .||::||||.||||.|||:||.||:.|.|| ...:..||:.|.:::|||.|.| .||||:..|.:
Human   515 LYAMVGAAACLGGVTRMTVSLVVIMFELTG-GLEYIVPLMAAAVTSKWVADAFGKEGIYEAHIHL 578

  Fly   634 NHVPMLTWEPLPQYKGLKAREIL----SKPVICIKIRDSANY--IYEMLKKCDHNGFPVVDDVCG 692
            |..|.|..:....::.| |.:::    .:|.:.:..:||...  :..::|:.|:||||||..
Human   579 NGYPFLDVKDEFTHRTL-ATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNGFPVVVS--- 639

  Fly   693 DRRSEGRVCGIILRSQLIVILLKSLYVENKRFWLPETSIQTFRDLYPRFPSIKSVRKLDEKINYT 757
              |...|:.|...|.:||:.:..:   ..::..:...||..|.:..|..|:         ...:.
Human   640 --RDSERLIGFAQRRELILAIKNA---RQRQEGIVSNSIMYFTEEPPELPA---------NSPHP 690

  Fly   758 VDLSMFMNPSPIRVNPHDSVPRIFQIFRALGLRHLLVINHENRIAGIITRRDFI 811
            :.|...:|.||..|..|..:..:..|||.||||..|| ....|:.||||::|.:
Human   691 LKLRRILNLSPFTVTDHTPMETVVDIFRKLGLRQCLV-TRSGRLLGIITKKDVL 743

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ClC-b	NP_610798.1	CBS_pair_EriC_assoc_euk_bac	674..811	CDD:239964	40/136 (29%)
		ClC_6_like	115..639	CDD:239657	178/619 (29%)
		Voltage_CLC	213..620	CDD:279048	145/455 (32%)
		CBS	653..811	CDD:223591	43/163 (26%)
CLCN4	NP_001821.2	Required for localization in the endoplasmic reticulum. /evidence=ECO:0000269\|PubMed:17023393	14..63		8/56 (14%)
		ClC_3_like	78..584	CDD:239656	169/570 (30%)
		Selectivity filter part_1. /evidence=ECO:0000250	180..184		3/3 (100%)
		Selectivity filter part_2. /evidence=ECO:0000250	222..226		3/3 (100%)
		Selectivity filter part_3. /evidence=ECO:0000250	467..471		2/3 (67%)
		CBS_pair_voltage-gated_CLC_euk_bac	595..745	CDD:341367	45/168 (27%)
		CBS repeat	601..696	CDD:341367	23/111 (21%)
		Required for localization in the endoplasmic reticulum. /evidence=ECO:0000269\|PubMed:28972156	667..696		6/37 (16%)
		CBS repeat	702..744	CDD:341367	17/43 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0038
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000220
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.810