Protein Alignment Cyp301a1 and cyp27a1.4

DIOPT Version :9

• Sequence 1: NP_610796.1
• Gene: Cyp301a1 / 36378
• FlyBaseID: FBgn0033753
• Length: 553
• Species: Drosophila melanogaster
• Sequence 2: NP_001315442.1
• Gene: cyp27a1.4 / 322341
• ZFINID: ZDB-GENE-030131-1060
• Length: 522
• Species: Danio rerio
• Alignment Length: 548
• Identity: 142/548 - (25%)
• Similarity: 229/548 - (41%)
• Gaps: 102/548 - (18%)

  Fly    13 VSCGPNLRQCV--PRISGAGSRRAQCRESSTGVATCP-HLADSEEASAPRI-------------- 60
            :|....|..|.  |..:..|.|||....::..|:... |.....||..|.|
Zfish     7 LSSAERLGWCFLRPTTAATGFRRAAGNSAAASVSVQDGHRKLKTEADLPEIKIFTMLYQMLFKGY 71

  Fly    61 -HSTSEWQNALPYNQIPGPKPIPILGNTWRLMPIIGQYTISDVAKISSLLHDRYGRIVRFGGLIG 124
             :|..|.|  |...|:.||        .|:                           :..|.|.|
Zfish    72 LNSVHELQ--LYQKQVYGP--------LWK---------------------------INAGNLQG 99

  Fly   125 RPDLLFIYDADEIEKCYRSEGPTPFRPSMPSLVKYKSVVRKDFFGDLGGVVGVHGEPWREFRSRV 189
                :.|...:.:|:..|.:...|.|..|....::     :|..|...|.....||.|.:.|:.:
Zfish   100 ----ISITSVELLEELLRKDEKYPCRGYMTLWTEH-----RDLRGISYGPFTEEGEKWYKLRAVL 155

  Fly   190 QKPVLQLSTIRRYLQPLEVITEDFLVRCENL--LDENQELPEDFDNEIHKWSLECIGRVALDTRL 252
            .|.:|......:|...:..:..||:.|...|  :....:|..:..||::::|||.|..:..:||:
Zfish   156 NKRMLHPKDSLQYGDVVNAVITDFIKRIYYLREMSPTGDLVSNLTNELYRFSLEGIASILFETRI 220

  Fly   253 GCLESNLKPDSEPQQIIDAAKYAL---RNVATLELKAPYWRYFPTPLWTRYVKNMNFFVGVCMKY 314
            ||||..:  .:|.|:.|::.....   .:||.|    |.|.....|.|.:|:...:.......|.
Zfish   221 GCLEKEI--PAETQEFINSIAQMFTYNMHVALL----PNWTRNYLPFWQKYIDGWDGIFKFGTKM 279

  Fly   315 IQSATERLKTQ-DPSLR-AGE--PSLVEKVILSQKDEKIATIMALDLILVGIDTISMAVCSMLYQ 375
            |....|.::|: |.:.. |||  ..|:....:|.||   ......:::|.|:||.|..:...||.
Zfish   280 INLKMEAIQTRLDTNQEVAGEYLTYLLSSGKMSCKD---VYGSVSEVLLAGVDTTSNTMLWALYL 341

  Fly   376 LATRPVDQQKVHEELKRLLPDPNTPLTIPLLDQMHHLKGFIKEVFRMYSTVIGNGRTLMEDSVIC 440
            |:..|..|:.:|:|:.::|.....| |...::.|..||..|||..|:|..|..|.|.:.|..||.
Zfish   342 LSKDPAAQETLHQEVTKVLKGDRIP-TAEEVNSMPFLKAVIKETLRLYPVVPVNSRLIAESEVII 405

  Fly   441 GYQVPKGVQAVFPTIVTGNM--------EEYVTDAATFRPERWLKPQHGGTPGKLHPFASLPYGY 497
            |       :.:||...|.|:        |:...:...|:|||||:  .|.|  :.:||.|:|:|:
Zfish   406 G-------EYLFPKKTTFNLFHYAISHDEKVFPEPQKFKPERWLR--DGRT--RPNPFGSIPFGF 459

  Fly   498 GARMCLGRRFADLEMQILLAKLLRNYKL 525
            |.|.|:|||.|:|||.:.||:|::.:::
Zfish   460 GVRACVGRRIAELEMHLALARLIKLFEM 487

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cyp301a1	NP_610796.1	p450	76..530	CDD:299894	123/467 (26%)
cyp27a1.4	NP_001315442.1	p450	66..505	CDD:278495	128/489 (26%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	217	1.000	Domainoid score	I2609
eggNOG	1	0.900	-	-		E2759_KOG0159
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	219	1.000	Inparanoid score	I3547
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D185685at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm6352
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X156
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	7	6.870