DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Cyp301a1 and Cyp27a1

DIOPT Version :9

Sequence 1:	NP_610796.1	Gene:	Cyp301a1 / 36378	FlyBaseID:	FBgn0033753	Length:	553	Species:	Drosophila melanogaster
Sequence 2:	NP_077226.2	Gene:	Cyp27a1 / 104086	MGIID:	88594	Length:	533	Species:	Mus musculus

Alignment Length:	557	Identity:	153/557 - (27%)
Similarity:	246/557 - (44%)	Gaps:	92/557 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 LKAWRSTVSCGPNLRQCV--PRISGAGSRRAQCRESSTGVATCPHLADSEEAS--------APRI 60
            :.||..|     .||..:  ||:.|    |..|.:.:...||.|....::|::        .||:
Mouse     1 MAAWSRT-----RLRWTLLDPRVVG----RGLCPQGARAKATIPAALQAQESTEGPGTGQDRPRL 56

  Fly    61 HSTSEWQNALPYNQIPGPKPIPILGNTWRLMPIIGQYTISDVAKISSLLHDRYGRI--VRFGGLI 123
            .|.:|         :||      .|....|..:..|..:..:..:..|...:||.:  ..||...
Mouse    57 RSPAE---------LPG------TGTLQFLFQLFLQGYVLHLPDLQVLNKTKYGPMWTTSFGTYT 106

  Fly   124 GRPDLLFIYDADEIEKCYRSEGPTPFRPSMPSLVKYKSVVRKDFFGDLGGVVGVHGEPWREFRSR 188
            .    :.:..|..:|:..|.||..|.|..|.   ::|.  .:|..|...|:....||.|...|..
Mouse   107 N----VNLASAPLLEQVMRQEGKYPIRDHMD---QWKD--HRDHKGLTYGIFIAQGEQWYHLRQA 162

  Fly   189 VQKPVLQLSTIRRYLQPLEVITEDFLVRCENLLDENQ---ELPEDFDNEIHKWSLECIGRVALDT 250
            :::.:|:......|...|..:..||:.|.:.:..|::   ::| |..:.::..:||.|..:..:.
Mouse   163 LKQRLLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVP-DMAHLLYHLALEAITYILFEK 226

  Fly   251 RLGCLESNLKPDSEPQQIIDAAKYALRNVATLELKAPYWRYFP------TPLWTRYVKNMNFFVG 309
            |:||    ||| |.|:   |.|.: :|:||.:...:.|..:.|      .|.|.||:...:....
Mouse   227 RIGC----LKP-SIPE---DTAAF-IRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNIFS 282

  Fly   310 VCMKYIQSATERLKTQDPSLRAGEPS----------LVEKVILSQKDEKIATIMALDLILVGIDT 364
            ...|.|....:.||.|   |:...|.          |:...:||.: |.|.|..  :|:|.|:||
Mouse   283 FGKKLIDEKVQELKAQ---LQETGPDGVRVSGYLHFLLTNELLSTQ-ETIGTFP--ELLLAGVDT 341

  Fly   365 ISMAVCSMLYQLATRPVDQQKVHEELKRLLPDPNTP-----LTIPLLDQMHHLKGFIKEVFRMYS 424
            .|..:...||.|:..|..|:.:|:|:..::|....|     ..:||      ||..|||..|:|.
Mouse   342 TSNTLTWALYHLSKSPEIQEALHKEVTGVVPFGKVPQHKDFAHMPL------LKAVIKETLRLYP 400

  Fly   425 TVIGNGRTLME-DSVICGYQVPKGVQAVFPTIVTGNMEEYVTDAATFRPERWLKPQHGGTPGKLH 488
            .|..|.|.:.| ::.|.|:..||..|.|....|.........:..:|:|.|||:.:....||.||
Mouse   401 VVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPNSFQPHRWLRKKEADNPGILH 465

  Fly   489 PFASLPYGYGARMCLGRRFADLEMQILLAKLLRNYKL 525
            ||.|:|:|||.|.|||||.|:||||::|::|::.|::
Mouse   466 PFGSVPFGYGVRSCLGRRIAELEMQLMLSRLVQKYEI 502

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Cyp301a1	NP_610796.1	p450	76..530	CDD:299894	135/477 (28%)
Cyp27a1	NP_077226.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	34..60		5/25 (20%)
		p450	82..527	CDD:278495	130/452 (29%)
		Sterol-binding. /evidence=ECO:0000255	386..400		8/19 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	190	1.000	Domainoid score	I3255
eggNOG	1	0.900	-	-		E2759_KOG0159
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	196	1.000	Inparanoid score	I3800
Isobase	1	0.950	-	0	Normalized mean entropy	S4770
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8705
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X156
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	7	6.810