Protein Alignment Polr3B and rpoB

DIOPT Version :10

• Sequence 1: NP_523706.1
• Gene: Polr3B / 36289
• FlyBaseID: FBgn0004463
• Length: 1137
• Species: Drosophila melanogaster
• Sequence 2: NP_418414.1
• Gene: rpoB / 948488
• ECOCYCID: EG10894
• Length: 1342
• Species: Escherichia coli
• Alignment Length: 1456
• Identity: 306/1456 - (21%)
• Similarity: 498/1456 - (34%)
• Gaps: 485/1456 - (33%)

  Fly    32 TEKWKL------VPAFLQVKGLVKQHIDSFNHFINVD----------IKKIVKANELVTSGADPL 80
            |||.::      .|..|.|..|:...:|||..||..|          .:.:......  ||...|
E. coli     6 TEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSY--SGNSEL 68

  Fly    81 FYLKYLDVRVGKPDIDDGFNITKATTPHECRLRDTTYSAPITVDI-------EYTRGTQR-IKRN 137
            .|:.|   |:|:|..|          ..||::|..|||||:.|.:       |...||.: ||..
E. coli    69 QYVSY---RLGEPVFD----------VQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ 120

  Fly   138 NLLIGRMPLMLRCSNCALTGKSEFELSKLNECPLDPGGYF-----VVRGQEKVI----LIQEQLS 193
            .:.:|.:|||.......:.|.....:|:|:.   .||.:|     ......||:    :|..:.|
E. coli   121 EVYMGEIPLMTDNGTFVINGTERVIVSQLHR---SPGVFFDSDKGKTHSSGKVLYNARIIPYRGS 182

  Fly   194 WNKMLTEDFNGVVQCQVTSSTHEKKSRTLVLSKHGKYYLKHNSMTDDIPIVVIFKALGVVSDQEI 258
            |   |..:|:......|......|...|::|.       ..|..|:.|..:...|.:..:.|.::
E. coli   183 W---LDFEFDPKDNLFVRIDRRRKLPATIILR-------ALNYTTEQILDLFFEKVIFEIRDNKL 237

  Fly   259 QSLIGIDSKSQNRFGASLIDAYNLKVFTQQ---------RAL------------EYMGSKLVVKR 302
            |..: :..:.:....:..|:| |.||:.::         |.|            ||:..|:|.|.
E. coli   238 QMEL-VPERLRGETASFDIEA-NGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKD 300

  Fly   303 FQSATT-------------------KTPSEEARELLLTTILAHVP-------VDNFNLQMKA-IY 340
            :...:|                   .....:..|.|.|..|.|.|       ||..|.::.| :.
E. coli   301 YIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVE 365

  Fly   341 VSMMVR------RVMAAELDKTLF----------------------------------------- 358
            :..|:|      |..|..|.:.||
E. coli   366 IYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMK 430

  Fly   359 ------------DDRDYYGNKRLELAGSLLSMMFEDLFKRMNWELKTIADKNIPKVKAAQFDVV- 410
                        ||.|:.||:|:...|.:....|.....|:...:|       .::.....|.:
E. coli   431 KLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVK-------ERLSLGDLDTLM 488

  Fly   411 --KHMRAAQITAGLESAISSGNWTIKRFKMERAGVTQVLSRLSYISALGM--MTRVNSQFEKTRK 471
              ..:.|..|:|.::....|..  :.:| |::......::....|||||.  :||..:.||
E. coli   489 PQDMINAKPISAAVKEFFGSSQ--LSQF-MDQNNPLSEITHKRRISALGPGGLTRERAGFE---- 546

  Fly   472 VSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTEVE--ERPVMIVAFNAGVEDIREVS 534
               .|.:.|:.:|.:||.:||||...||:.:|::... |.|..  |.|...|......::|..:|
E. coli   547 ---VRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQ-TNEYGFLETPYRKVTDGVVTDEIHYLS 607

  Fly   535 GNPINNPNVFLVFINGNVLGLTLNHK-HLVRNLRYMRRKGRMGSYVSVHTSY----TQRCIYI-- 592
            .  |...|..:...|.|     |:.: |.|.:|...|.||....:......|    ||:.:.:
E. coli   608 A--IEEGNYVIAQANSN-----LDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGA 665

  Fly   593 -------HTD------GGRLCRPYV-IVENRRPLV-----KQHHLDELNRGIRKFDDFLLDGLIE 638
                   |.|      |..:.|..| .:...:|||     :...:|.....:.|     ..|:::
E. coli   666 SLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAK-----RGGVVQ 725

  Fly   639 YLDVNEENDSFIAWNEDQIEDRTTHLEIEPFTLLGVCAGLVPYPHHNQSPRNTYQCAMGKQAMGM 703
            |:|.:.   ..|..|||::......::|         ..|..|...||   ||....|...::|
E. coli   726 YVDASR---IVIKVNEDEMYPGEAGIDI---------YNLTKYTRSNQ---NTCINQMPCVSLG- 774

  Fly   704 IGYNQKNRIDSLMYNLVYPHAPMVKSKTI---ELTNFDKLPAGQNATVAVMSYSGYDIEDALILN 765
                                .|:.:...:   ..|:..:|..|||..||.|.::||:.||:::::
E. coli   775 --------------------EPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVS 819

  Fly   766 KASIDRGYGRCLVYKNSKCTVKRYANQTFDRIMGP---------MKDALTNKVIFKHDVLDTDGI 821
            :..:.......:..:...|..:       |..:||         :.:|..:|       ||..||
E. coli   820 ERVVQEDRFTTIHIQELACVSR-------DTKLGPEEITADIPNVGEAALSK-------LDESGI 870

  Fly   822 VAPGEQVQNKQIMINKEMP-AVTSMNP---------------LQGQSAQVPY----TAVPI---S 863
            |..|.:|....|::.|..| ..|.:.|               ::..|.:||.    |.:.:   :
E. coli   871 VYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFT 935

  Fly   864 YKGPEP-------------------------------SYIERVMVSANAEEDFL----------- 886
            ..|.|.                               |.|..|:|:...|.:.|
E. coli   936 RDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLEL 1000

  Fly   887 -------------------------------------------------IKILLRQTRIPEIGDK 902
                                                             :|:.|...|..:.|||
E. coli  1001 GLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDK 1065

  Fly   903 FSSRHGQKGVTGLIVEQEDMPFNDFGICPDMIMNPHGFPSRMTVGKTLELLGGKAG--------- 958
            .:.|||.|||...|...||||:::.|...|:::||.|.||||.:|:.||...|.|.
E. coli  1066 MAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINA 1130

  Fly   959 -------------LLEGKFHYGT--------------------------------AFGGSKVEDI 978
                         .::..:..|.                                .|.|:|..:|
E. coli  1131 MLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEI 1195

  Fly   979 QAELERHGFNYVGKDFFYSGITGTPLEAYIYSGPVYYQKLKHMVQDKMHARARGPKAVLTRQPTQ 1043
            :..|:.......|:...|.|.||...|..:..|.:|..||.|:|.||||||:.|..:::|:||..
E. coli  1196 KELLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG 1260

  Fly  1044 GRSREGGLRLGEMERDCLISYGASMLIMERLMISSDAFEVDVCRTCGRMAYCSWCHFCQSSANV- 1107
            |:::.||.|.||||...|.:|||:..:.|.|.:.||    ||   .||         .:...|:
E. coli  1261 GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSD----DV---NGR---------TKMYKNIV 1309

  Fly  1108 -----SKISMPYACKLLFQELTSMNV 1128
                 .:..||.:..:|.:|:.|:.:
E. coli  1310 DGNHQMEPGMPESFNVLLKEIRSLGI 1335

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr3B	NP_523706.1	RNA_pol_B_RPB2	47..1133	CDD:238353	300/1435 (21%)
		RNA_pol_Rpb2_4	546..607	CDD:428012	17/81 (21%)
		RNA_pol_Rpb2_5	628..668	CDD:428013	8/39 (21%)
rpoB	NP_418414.1	rpoB	1..1342	CDD:234749	306/1456 (21%)
		HMG-box_SF	972..1029	CDD:469606	6/56 (11%)