Protein Alignment Polr3B and polr2b

DIOPT Version :10

• Sequence 1: NP_523706.1
• Gene: Polr3B / 36289
• FlyBaseID: FBgn0004463
• Length: 1137
• Species: Drosophila melanogaster
• Sequence 2: NP_001019632.2
• Gene: polr2b / 100149268
• ZFINID: ZDB-GENE-041008-1
• Length: 1174
• Species: Danio rerio
• Alignment Length: 1164
• Identity: 438/1164 - (37%)
• Similarity: 686/1164 - (58%)
• Gaps: 83/1164 - (7%)

  Fly    35 WKLVPAFLQVKGLVKQHIDSFNHFINVDIKKIV--------KANELVTSG---ADPLFYLKYLDV 88
            |.::.::...||||:|.:|||:.||.:.:::||        :|....|||   ..|.:.||:..:
Zfish    27 WIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHTSGEVEEPPRYLLKFEQI 91

  Fly    89 RVGKPDIDDGFNITKATTPHECRLRDTTYSAPITVDIEYT-----RGTQRIKRNNLLIGRMPLML 148
            .:.||...:.........|:|.|||:.|||||:.|||..|     ...|:.:.....||::|:||
Zfish    92 YLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTIIKDGEEQQQTQHQKTFIGKIPIML 156

  Fly   149 RCSNCALTGKSEFELSKLNECPLDPGGYFVVRGQEKVILIQEQLSWNKMLT---ED----FNGVV 206
            |.:.|.|:|.::.:|.:||||||||||||::.|.|||::.||:::.|.:..   :|    :.|
Zfish   157 RSTYCLLSGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTG-- 219

  Fly   207 QCQVTSSTHEKKSRTLVLS--KHGKYYLKHNS-----------MTDDIPIVVIFKALGVVSDQEI 258
            :|:.......:.:.|:.:|  ..|...:|.::           :..::||:::|:|||.|||::|
Zfish   220 ECRSCLENSSRPTSTIWVSMMARGGQGVKKSAIGQRIVSTLPYIRQEVPIIIVFRALGFVSDRDI 284

  Fly   259 QSLIGI---DSKSQNRFGASLIDAYNLKVFTQQRALEYMGSKLVVKRFQSATTKTPSEEARELLL 320
            ...|..   |.:.......||.:|:.::  .|..||.::||:   ......|.:...:.|:|:|.
Zfish   285 LEHIIYDFDDPEMMEMVKPSLDEAFVIQ--EQNVALNFIGSR---GAKPGVTKERRIKYAKEVLQ 344

  Fly   321 TTILAHVPVDNFNLQMKAIYVSMMVRRVMAAELDKTLFDDRDYYGNKRLELAGSLLSMMFEDLFK 385
            ..:|.||.|.:|....||.::..||.|::.|.|.:...||||:||||||:|||.||:.:|..:||
Zfish   345 KEVLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFK 409

  Fly   386 RMNWELKTIADKNIPKVKAAQFDVVKHMRAAQITAGLESAISSGNW-TIKRFKMERAGVTQVLSR 449
            .:..|::..|.|.|.:.|  .|::...::...|:.||:.::::||| .:|:....||||:|||:|
Zfish   410 NLLKEIRIYAQKFIDRGK--DFNLELAIKTRIISDGLKYSLATGNWGDVKKAHQARAGVSQVLNR 472

  Fly   450 LSYISALGMMTRVNSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTEVE 514
            |::.|.|..:.||||...:..|::.||.|..:.|||:||::||||.|.|||||||||.:|:...:
Zfish   473 LTFASTLSHLRRVNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQ 537

  Fly   515 ERPVMIVAFNAGVEDIREVSGNPINNPNVFLVFINGNVLGLTLNHKHLVRNLRYMRRK-GRMGSY 578
            ..|::.......:|::.|:|  |....:...:|:||..:|:..:.:.|:..||.:||: ..:.|.
Zfish   538 PSPILEFLEEWSMENLEEIS--PAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSE 600

  Fly   579 VSVHTSYTQRCIYIHTDGGRLCRPYVIVENRRPLVKQHHLDELNRGIRKFD-----DFLLDGLIE 638
            ||:.....:|.|.|:||.||:|||.:|||.::.|:|:.|:|:|..  |:::     |.:..|::|
Zfish   601 VSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKRRHIDQLKE--REYNNYSWQDLVASGVVE 663

  Fly   639 YLDVNEENDSFIAWNEDQIEDR-------TTHLEIEPFTLLGVCAGLVPYPHHNQSPRNTYQCAM 696
            |:|..||....:|...|.::::       .||.||.|..:|||||.::|:|.||||||||||.||
Zfish   664 YIDTLEEETVMLAMTPDDLQEKGVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAM 728

  Fly   697 GKQAMGMIGYNQKNRIDSLMYNLVYPHAPMVKSKTIELTNFDKLPAGQNATVAVMSYSGYDIEDA 761
            ||||||:...|...|:|:|.:.|.||..|:|.::::|...|.:||||.|:.||:.||:||:.||:
Zfish   729 GKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDS 793

  Fly   762 LILNKASIDRGYGRCLVYKNSKCTVKRYANQTFDR---IMGPMKDALTNKVIFKHDVLDTDGIVA 823
            :|:|::::|||:.|.:.|::.|   ::.:.:.||:   ...|.::.........:|.||.||::|
Zfish   794 VIMNRSAVDRGFFRSVFYRSYK---EQESKKGFDQEEIFEKPTRETCQGMRHAIYDKLDDDGLIA 855

  Fly   824 PGEQVQNKQIMINKEMPAVTSMNPLQGQSAQVPYTAVPIS--YKGPEPSYIERVMVSANAEEDFL 886
            ||.:|..:.::|.|.:....:.:.|...:.:  ||....|  .:..|...:::|||:.|.|....
Zfish   856 PGVRVSGEDVIIGKTVTLPENDDELDSTNRR--YTKRDCSTFLRTSETGIVDQVMVTLNQEGYKF 918

  Fly   887 IKILLRQTRIPEIGDKFSSRHGQKGVTGLIVEQEDMPFNDFGICPDMIMNPHGFPSRMTVGKTLE 951
            .||.:|..|||:|||||:|||||||..|:...||||||...||.||:|:|||..|||||||..:|
Zfish   919 CKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTVGHLIE 983

  Fly   952 LLGGKAGLLEGKFHYGTAFGGS-KVEDIQAELERHGFNYVGKDFFYSGITGTPLEAYIYSGPVYY 1015
            .|.||....:|:....|.|..: .|:.:...|..:|::..|.:..|:|.||..|.:.|:.||.||
Zfish   984 CLQGKVSANKGEIGDATPFNDAVNVQKVSNLLSEYGYHLRGNEVLYNGFTGRKLTSQIFVGPTYY 1048

  Fly  1016 QKLKHMVQDKMHARARGPKAVLTRQPTQGRSREGGLRLGEMERDCLISYGASMLIMERLMISSDA 1080
            |:|||||.||:|:|||||..:|.|||.:||||:||||.|||||||.|::||:..:.|||..:||.
Zfish  1049 QRLKHMVDDKIHSRARGPVQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLKERLFEASDP 1113

  Fly  1081 FEVDVCRTCGRMAYCSW------CHFCQSSANVSKISMPYACKLLFQELTSMNVVPKMI 1133
            ::|.||..||.||..:.      |..|::...:|.:.||||||||||||.||::.|:|:
Zfish  1114 YQVHVCNLCGLMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMM 1172

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr3B	NP_523706.1	RNA_pol_B_RPB2	47..1133	CDD:238353	434/1150 (38%)
		RNA_pol_Rpb2_4	546..607	CDD:428012	23/61 (38%)
		RNA_pol_Rpb2_5	628..668	CDD:428013	13/51 (25%)
polr2b	NP_001019632.2	Ricin_B_lectin	27..1172	CDD:481363	437/1162 (38%)