DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment MCPH1 and Brca1

DIOPT Version :9

Sequence 1:	NP_001163123.1	Gene:	MCPH1 / 36272	FlyBaseID:	FBgn0260959	Length:	1028	Species:	Drosophila melanogaster
Sequence 2:	NP_033894.3	Gene:	Brca1 / 12189	MGIID:	104537	Length:	1812	Species:	Mus musculus

Alignment Length:	1214	Identity:	233/1214 - (19%)
Similarity:	397/1214 - (32%)	Gaps:	375/1214 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly    37 RPLHDS--NLGDFRSKAAAPSPK---------SLSQAPADDQAARDRLMLKPALAVSKA------ 84
            |.:.||  .||| |.....||.|         |:|.....|...::.:.:..|..|..|
Mouse   729 RQMSDSAKELGD-RVLGGEPSGKTTDRSEESTSVSLVSDTDYDTQNSVSVLDAHTVRYARTGSAQ 792

  Fly    85 ------ISQHRSEFHQDENEAGAEGSAAKPDPPIGAATPTRQEGHWARLQQD-LNSPNAALRLRA 142
                  .|::..|.....|.||:.....||        |.|   |...|.|: :...::.|
Mouse   793 CMTQFVASENPKELVHGSNNAGSGTEGLKP--------PLR---HALNLSQEKVEMEDSEL---- 842

  Fly   143 IRALKSPTKSAYNTFDVPLAEQSIITMEERNPQE--PPSLPELLKDIVVYVEVRTGNDNRSEG-- 203
                  .|:...|||.|...:...:..:.|:||:  ..|:|.  |::...|..: |.....:|
Mouse   843 ------DTQYLQNTFQVSKRQSFALFSKPRSPQKDCAHSVPS--KELSPKVTAK-GKQKERQGQE 898

  Fly   204 ------VKSIIAKMGAQVK------------DRLTR----------------------------- 221
                  |:::.|.:|..|.            ||..|
Mouse   899 EFEISHVQAVAATVGLPVPCQEGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFK 963

  Fly   222 -----------TTTHVVFKDGLLSTYKKATEWNIPVVSILWIEACKVQL---------------- 259
                       |.......:|....:..:||..:...:||......|.|
Mouse   964 QSVSPIRSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNACQEAGSGSIH 1028

  Fly   260 KICDPKQFPISNIRMYEYPELFGK--------MPRVKFMQPD--------SELNQRPRKRQG--- 305
            ::|....         .:|...|:        :|.:..|||.        |:.....:|::|
Mouse  1029 EVCSTGD---------SFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPVSDKYLEIKKQEGEAV 1084

  Fly   306 ----TPTSSKDN-EPGSTNKRAQTGTPTTTTPKNDISRFFRAINHNKPSDDEAIESPAT---KLL 362
                :|....|: |...:.|..|..:.|.....:|:    ....|....:.:.:|..|.   .:|
Mouse  1085 CADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDDV----EIQGHTSFGEGDIMERSAVFNGSIL 1145

  Fly   363 NRISSGCYTPLPAGGTPQLMGTNGSGDAVAQKVQAQKSLQFGEQTDSNTPQAKPSSTSRPRRSTV 427
            .|.||...:|:......|.:.            :|.:.|:..|::||...:..|.......|.:.
Mouse  1146 RRESSRSPSPVTHASKSQSLH------------RASRKLESSEESDSTEDEDLPCFQHLLSRISN 1198

  Fly   428 DPPPVMTPPVRTTRRRSCVAEISSPAATDTRMTRRRSS--LLTSTVIHEADPTQTAVAEPRMTRR 490
            .|        ..||..|.|.:.....|..|:...:.||  .....::.||........:||.:..
Mouse  1199 TP--------ELTRCSSAVTQRMPEKAEGTQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGS 1255

  Fly   491 RSSLLGIAKQ---AEETAQRQTIQPITEELAPVEENRNSIIMAETNIINN---TNCLEQSIEITA 549
            ..|....|.|   |...:|.....|.:::.:....|..:.:..:..|.:|   ..|||:..:
Mouse  1256 MFSSQHSAAQGSTANANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDND--- 1317

  Fly   550 VEAEASFKADRRGTLYASEL--------MDISSPRRRSVAKSSL-ELRQEN------LAPRARQS 599
             :.|.|...|...:.|.||.        .||.:.::|:..|.:| :|:||.      |..|..|.
Mouse  1318 -QEEDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAHLEAVLEQRGNQP 1381

  Fly   600 -------LSNVTTQTSFLDVEQQVTPLFSSTRLPTGQTTGNRRRTIFNMDMDVINESIERMNQSH 657
                   |::........|:|    |..|...:.|.:.               |||:    ..|.
Mouse  1382 SGHSPSLLADPCALEDLPDLE----PNMSGAAILTSKN---------------INEN----PVSQ 1423

  Fly   658 RMSLALANKAELG--ESCQVHEQEPPQAEPVPSQEP----------------KRRRLFNPNDEVV 704
            .:..|..:|.:|.  |.....:.|.....|.|.:.|                ||.   :|:.|.:
Mouse  1424 NLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHGCSRHLQKRN---SPSQEEL 1485

  Fly   705 ISPAMSNRKSRLSITGSGSSSSVQKRRRSLATPAKSSSSEL----------------CATTPLS- 752
            :.||.|...|.   ..:.:..|...||....||...|...|                ..|||.|
Mouse  1486 LQPAGSEASSE---PHNSTGQSCLPRRELEGTPYLGSGISLFSSRDPESESPKEPAHIGTTPAST 1547

  Fly   753 ---KAVTNKEAEKSKSNTEISEASLDAVPEVKKSTSNAGEENEKPKGKSRTRIRTLVHTNMHQEQ 814
               |....:.|.:|.:.....:|.:..|.::|...:::.|..:        |..::|.:.:..::
Mouse  1548 SALKIPQGQVAFRSAAAAGADKAVVGIVSKIKPELTSSEERAD--------RDISMVVSGLTPKE 1604

  Fly   815 INVIRKALRKLRGMRLDPTVTQHTTHLV-----SLEPRRTLNLLRGLMRGVWIVSYQWVLASIRA 874
            :..::|...|.| :.|...:|:.|||::     .....|||....|:..|.|||||.||:.||:.
Mouse  1605 VMTVQKFAEKYR-LTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQE 1668

  Fly   875 GKWINEEPYELT------RFSRAIEICRTERQAFGVHYQCELFRFMEPFYVSSLCRPVQFNNM-- 931
            .:.:|...:|:.      |..:.....|..|:        :||:.::.:    .|.|  |.||
Mouse  1669 RRLLNVHEFEVKGDVVTGRNHQGPRRSRESRE--------KLFKGLQVY----CCEP--FTNMPK 1719

  Fly   932 ---KELLLLGGATL------------------------TENR--------FKAKYIIGDKRRAED 961
               :.:|.|.||::                        ||:.        .||:.::.|
Mouse  1720 DELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQLCKARLVMWD------ 1778

  Fly   962 DRVYLDPYWVLDSITNMQIQRFGKYLMKSAIISADGIRYEDPRD 1005
                    |||||:::.:.:....||:::  |:.|....:|..|
Mouse  1779 --------WVLDSLSSYRCRDLDAYLVQN--ITCDSSEPQDSND 1812

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
MCPH1	NP_001163123.1	PTCB-BRCT	187..251	CDD:289507	15/123 (12%)
MCPH1	NP_001163123.1	BRCT	812..873	CDD:237994	21/65 (32%)
Brca1	NP_033894.3	rad18	15..>172	CDD:273165
		RING-HC_BRCA1	18..65	CDD:319412
		RING-HC finger (C3HC4-type)	24..64	CDD:319412
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	165..198
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	321..362
		BRCT_assoc	342..503	CDD:372330
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	492..581
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	640..767		12/38 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	864..899		9/37 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	947..995		3/47 (6%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1030..1056		4/34 (12%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1147..1185		10/49 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1205..1230		6/24 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1244..1289		8/44 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1313..1343		7/33 (21%)
		Interaction with PALB2. /evidence=ECO:0000250	1353..1380		8/26 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1437..1547		23/115 (20%)
		BRCT_BRCA1_rpt1	1593..1689	CDD:349367	26/96 (27%)
		BRCT_BRCA1_rpt2	1700..1797	CDD:349353	22/124 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4362
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900