DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment ERp60 and pdia8

DIOPT Version :9

Sequence 1:	NP_725084.2	Gene:	ERp60 / 36270	FlyBaseID:	FBgn0033663	Length:	489	Species:	Drosophila melanogaster
Sequence 2:	NP_001003517.1	Gene:	pdia8 / 445123	ZFINID:	ZDB-GENE-040801-20	Length:	493	Species:	Danio rerio

Alignment Length:	483	Identity:	221/483 - (45%)
Similarity:	306/483 - (63%)	Gaps:	16/483 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 LAGVLLLGFIAISSGAEQ--DVLELGDDDFATTLKQHETTLVMFYAPWCGHCKRLKPEYAKAAEI 67
            |.|:|.:...::||.|.:  |||:|.|.||.....:|||.||.||||||||||:|.||:..||..
Zfish     6 LRGLLCILVCSLSSSAREHSDVLKLTDADFDYLAPEHETLLVKFYAPWCGHCKKLAPEFESAASR 70

  Fly    68 VKDDDPPIKLAKVDCTEAGKETCSKYSVSGYPTLKIFRQDEVSQDYNGPREASGIAKYMRAQVGP 132
            :|.   .:.||||||| |..|.|..|.|:|||||||||..:.|..|:|||.|.||..||:.|.||
Zfish    71 LKG---TVTLAKVDCT-ANTEICKHYGVNGYPTLKIFRNGQESSSYDGPRSADGIVDYMKKQAGP 131

  Fly   133 ASKTVRTVAELKKFLDTKDTTLFGYFSDSDSKLAKIFLKFADKNREKYRFGHSSEKEVLDKQGET 197
            .|..:.:..:|:||::..|.::.|.||.:||.....|||.|...||.:||.|:::.::..|.|.|
Zfish   132 DSVLLHSELDLEKFINHFDASVVGLFSGTDSSQLAEFLKGASLMRESFRFAHTTDLQLGQKYGVT 196

  Fly   198 -DKIVLIRAPHLSNKFESSSIKFEGS-SESDLSTFVKENFHGLVGHRTQDSVKDF-QNPLITAYY 259
             :.|:|.|||.||:|||.|.:...|| |.:.|..|:::|..||..|.|:|:.:.. :..|:||||
Zfish   197 HESILLFRAPRLSSKFEESVVPHTGSLSVTGLRRFIRDNIFGLCPHMTKDNKEVLRKRDLLTAYY 261

  Fly   260 SVDYQKNPKGTNYWRNRVLKVAKEFVGQ-INFAIASKDDFQHEL-NEYGYDFV--GDKPVVLARD 320
            .:||..||||:|||||||||||.:|..| :.|::|:::||..|| .|:|....  .:.|.|..|.
Zfish   262 DLDYLHNPKGSNYWRNRVLKVATKFSSQGMLFSVANRNDFMEELEEEFGLSASDGNELPFVTIRT 326

  Fly   321 EKNLKYALKDEFSVE--NLQDFVEKLLANELEPYIKSEPIPESNDAPVKVAVAKNFDDLVINNGK 383
            ....||::::||:.:  :|:.|:|...|..|:.|:||||:|..|:..|||.||..|:::|.:..|
Zfish   327 RTGDKYSMREEFTRDGKSLESFLEDYFAGRLKRYVKSEPVPAINNGVVKVVVADTFEEIVNDPEK 391

  Fly   384 DTLIEFYAPWCGHCKKLSPIYEELAEKL-QDEDVAIVKMDATANDVPPEFNVRGFPTLFWLPKDA 447
            |.|||||||||||||||.|.|..|.|.| .|.::.|.|||||.||||..::|:||||:::.....
Zfish   392 DVLIEFYAPWCGHCKKLEPKYTALGEMLYSDPNIVIAKMDATVNDVPAGYDVQGFPTIYFAAAGR 456

  Fly   448 KNKPVSYNGGREVDDFLKYIAKEATTEL 475
            |::|..|.|.|||.||:.::.:|||..|
Zfish   457 KSEPKRYEGAREVKDFVNFLKREATKPL 484

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ERp60	NP_725084.2	ER_PDI_fam	23..479	CDD:273457	215/463 (46%)
		PDI_a_PDIR	23..126	CDD:239295	57/102 (56%)
		PDI_b_ERp57	133..235	CDD:239367	38/103 (37%)
		PDI_b'_ERp72_ERp57	239..345	CDD:239371	45/112 (40%)
		PDI_a_PDI_a'_C	365..467	CDD:239293	54/102 (53%)
pdia8	NP_001003517.1	ER_PDI_fam	25..484	CDD:273457	214/462 (46%)
		pdi_dom	30..129	CDD:273454	56/102 (55%)
		PDI_b_ERp57	132..236	CDD:239367	38/103 (37%)
		PDI_b'_ERp72_ERp57	240..353	CDD:239371	45/112 (40%)
		PDI_a_PDI_a'_C	373..476	CDD:239293	54/102 (53%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170591998
Domainoid	1	1.000	147	1.000	Domainoid score	I4447
eggNOG	1	0.900	-	-		E2759_KOG0190
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	431	1.000	Inparanoid score	I1689
OMA	1	1.010	-	-		QHG53639
OrthoDB	1	1.010	-	-		D222522at33208
OrthoFinder	1	1.000	-	-		FOG0000292
OrthoInspector	1	1.000	-	-		otm26283
orthoMCL	1	0.900	-	-		OOG6_100478
Panther	1	1.100	-	-	O	PTHR18929
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X241
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.770