DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment en and EN1

DIOPT Version :9

Sequence 1:	NP_523700.2	Gene:	en / 36240	FlyBaseID:	FBgn0000577	Length:	552	Species:	Drosophila melanogaster
Sequence 2:	NP_001417.3	Gene:	EN1 / 2019	HGNCID:	3342	Length:	392	Species:	Homo sapiens

Alignment Length:	500	Identity:	159/500 - (31%)
Similarity:	214/500 - (42%)	Gaps:	137/500 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    62 PAMAFDAAAAAAAAAAAAAAHAHAAALQQRLSGSGSPASCSTPASSTPLTIKEEESDSVIGDMSF 126
            |....|:|..|||||.........:......|||||... |.|.|..|.. ....:...:..::.
Human     8 PKSQRDSALGAAAAATPGGLSLSLSPGASGSSGSGSDGD-SVPVSPQPAP-PSPPAAPCLPPLAH 70

  Fly   127 HNQTHTTNEEEEAEEDDDIDVDVDDTSAGGRLPP------------PAHQQQSTAK--PSLAFSI 177
            |                            ..|||            ||||.|..|:  .:..|.|
Human    71 H----------------------------PHLPPHPPPPPPQHLAAPAHQPQPAAQLHRTTNFFI 107

  Fly   178 SNILSDRFGDVQKPGKSMENQASIFRPFEASRSQTATPSAFTRVDLLEFSRQQQAAAAAATAAMM 242
            .|||...||                     .:.:...|             |...||||...|
Human   108 DNILRPDFG---------------------CKKEQPPP-------------QLLVAAAARGGA-- 136

  Fly   243 LERANFLNCFNPAAYPRIHEEIVQSRLRRSAANAVIPPPMSSKMSDANPEKSALGSLCKAVSQIG 307
                        ....|:..:..|:...|...:     |:.::.    |..::|  ||...:..|
Human   137 ------------GGGGRVERDRGQTAAGRDPVH-----PLGTRA----PGAASL--LCAPDANCG 178

  Fly   308 QPAAPTMTQPPLSSSASSLASPPPASNASTISSTSSVATSSSSSSSGCSSAASSLNSSPSSRLGA 372
            .|..   :||..:.:.:|.|..|              |.:::::::..::||::..:.||. .|.
Human   179 PPDG---SQPAAAGAGASKAGNP--------------AAAAAAAAAAVAAAAAAAAAKPSD-TGG 225

  Fly   373 SGSGVNASSPQPQPIPPPSAVSRDSGMESSDDTRSETGSTT------TEGGKNEMWPAWVYCTRY 431
            .|||..|.||..|....|         |..:......||..      |:..:..:||||||||||
Human   226 GGSGGGAGSPGAQGTKYP---------EHGNPAILLMGSANGGPVVKTDSQQPLVWPAWVYCTRY 281

  Fly   432 SDRPSSGPRYRRPKQPKDKTNDEKRPRTAFSSEQLARLKREFNENRYLTERRRQQLSSELGLNEA 496
            |||||||||.|:.|:.|::..| |||||||::|||.|||.||..|||:||:|||.|:.||.|||:
Human   282 SDRPSSGPRTRKLKKKKNEKED-KRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELSLNES 345

  Fly   497 QIKIWFQNKRAKIKKSTGSKNPLALQLMAQGLYNHTTVPLTKEEE 541
            |||||||||||||||:||.||.|||.|||||||||:|..:..::|
Human   346 QIKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKDE 390

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
en	NP_523700.2	Homeobox	457..510	CDD:278475	39/52 (75%)
en	NP_523700.2	Engrail_1_C_sig	512..541	CDD:287495	17/28 (61%)
EN1	NP_001417.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..100		27/121 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	132..164		6/54 (11%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	219..251		12/41 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	282..306		14/24 (58%)
		Homeobox	306..359	CDD:278475	39/52 (75%)
		Engrail_1_C_sig	361..390	CDD:287495	17/28 (61%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165144575
Domainoid	1	1.000	94	1.000	Domainoid score	I7480
eggNOG	1	0.900	-	-		E1_KOG0493
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002003
OrthoInspector	1	1.000	-	-		mtm8573
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR24341
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2569
SonicParanoid	1	1.000	-	-		X1139
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	10	9.830