DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment metro and PALS1

DIOPT Version :9

Sequence 1:	NP_610642.2	Gene:	metro / 36176	FlyBaseID:	FBgn0050021	Length:	595	Species:	Drosophila melanogaster
Sequence 2:	NP_071919.2	Gene:	PALS1 / 64398	HGNCID:	18669	Length:	675	Species:	Homo sapiens

Alignment Length:	603	Identity:	213/603 - (35%)
Similarity:	350/603 - (58%)	Gaps:	74/603 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 LSKLISTLKESENL----SNDQEIDFLKALLESKELNALVNVHTKV-AKVGRDDRLAPVLSTSAQ 75
            :..|.|:||..::.    .:.::|..|..|:::|:......:|..: ..:.:.....|::|.:..
Human   124 IEDLFSSLKHIQHTLVDSQSQEDISLLLQLVQNKDFQNAFKIHNAITVHMNKASPPFPLISNAQD 188

  Fly    76 VLYEVLEQLSQHCHLNDDCKEAFHLLQDSHLQHLLFAHDAIAQKDFYPHLPEAPVEMDEDE---- 136
            :..||...|....|  .:.:|...||...|:|.||.|||.:|::       |..:|...||
Human   189 LAQEVQTVLKPVHH--KEGQELTALLNTPHIQALLLAHDKVAEQ-------EMQLEPITDERVYE 244

  Fly   137 -------ETIKIVQLVKSNE-PLTGAQSTEPIVGATIKTDEESGKIIIARIMHGGAADRSGLIHV 193
                   ||:|||::.|:.: ||          |||::.:.:|  :||:||:.||||::|||:|.
Human   245 SIGQYGGETVKIVRIEKARDIPL----------GATVRNEMDS--VIISRIVKGGAAEKSGLLHE 297

  Fly   194 GDEVIEVNNINVEGKTPGDVLTILQNSEGTITFKLVPAD--NKGAQRESKVRVRAHFDYNPDVDP 256
            ||||:|:|.|.:.||...:|..:|.:..||:||.|:|:.  .....:|:.:.|:|||||:|..||
Human   298 GDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDP 362

  Fly   257 YIPCKEAGLAFQRGDVLHIVAQDDAYWWQARKE--HERSARAGLIPSRALQERRILHDRTQKNGT 319
            |:||:|.||:||:||:||:::|:|..||||.:|  .:....|||:|.::.|::|    ...|...
Human   363 YVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQR----EAMKQTI 423

  Fly   320 DLDSKPGSCASL-CTTPPGSPRLPASSSTSSCRQPKTKKIMYDLTENDDFDREQIATYEEVAKLY 383
            :.|.:|.....| |               :...:.|.||::|:..:|||:|.|:|.||||:: ||
Human   424 EEDKEPEKSGKLWC---------------AKKNKKKRKKVLYNANKNDDYDNEEILTYEEMS-LY 472

  Fly   384 PRP-GVFRPIVLIGAPGVGRNELRRRLIARDPEKFRSPVPYTTRPMRTGEVAGREYIFVAREKMD 447
            .:| ...|||:|||....|:||||:||:.::.::|.|.||:|||..|..|||||:|.||:|:..:
Human   473 HQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFE 537

  Fly   448 ADIEAGKFVEHGEYKGHLYGTSAESVKSIVNAGCVCVLSPHYQAIKTLRTAQLKPFLIHVKPPEL 512
            |||.||||:||||::.:|||||.:||:.::|:|.:|:||...|::||||.:.|||::|.:.||..
Human   538 ADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQ 602

  Fly   513 DILKATRTEARAKSTFDEANARSFTDEEFEDMIKSAERIDFLYGHFFDVELVNGELVNAFEQLVQ 577
            :.|:|...:          ..::...||..::|:....::...||:||..:||.:|..|:::|::
Human   603 ERLRALLAK----------EGKNPKPEELREIIEKTREMEQNNGHYFDTAIVNSDLDKAYQELLR 657

  Fly   578 NVQRLENEPVWAPSMWVQ 595
            .:.:|:.||.|.||.|::
Human   658 LINKLDTEPQWVPSTWLR 675

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
metro	NP_610642.2	L27	17..68	CDD:197794	9/55 (16%)
		L27	75..127	CDD:197794	15/51 (29%)
		PDZ_signaling	139..229	CDD:238492	37/90 (41%)
		SH3_MPP	243..302	CDD:212796	32/60 (53%)
		Guanylate_kin	390..575	CDD:279019	77/184 (42%)
		GuKc	399..580	CDD:214504	72/180 (40%)
PALS1	NP_071919.2	Required for the correct localization of PALS1 and PATJ at cell-cell contacts and the normal formation of tight junctions and adherens junctions. /evidence=ECO:0000250\|UniProtKB:Q9JLB2	1..345		71/241 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..34
		Interaction with PARD6B. /evidence=ECO:0000250\|UniProtKB:Q9JLB2	21..140		4/15 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..78
		L27_N	123..170	CDD:401122	9/45 (20%)
		Interaction with LIN7C. /evidence=ECO:0000250\|UniProtKB:Q9JLB2	181..243		21/70 (30%)
		L27	189..237	CDD:197794	17/56 (30%)
		PDZ_signaling	254..333	CDD:238492	37/90 (41%)
		SH3_MPP5	349..411	CDD:212969	32/61 (52%)
		GuKc	491..661	CDD:214504	72/179 (40%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0194
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D245552at33208
OrthoFinder	1	1.000	-	-		FOG0000824
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.820