DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Git and Git1

DIOPT Version :9

Sequence 1:	NP_610599.3	Gene:	Git / 36122	FlyBaseID:	FBgn0033539	Length:	731	Species:	Drosophila melanogaster
Sequence 2:	NP_114002.1	Gene:	Git1 / 83709	RGDID:	69331	Length:	770	Species:	Rattus norvegicus

Alignment Length:	807	Identity:	295/807 - (36%)
Similarity:	412/807 - (51%)	Gaps:	164/807 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    36 KMPRGKSRLQTEVCGDCGAGDPSWASINRGILLCADCCSVHRSLGRHISIVKSLRQGNWEPSVLN 100
            |.||      .|||.||.|.||.||||:||:|:|.:||||||||||||||||.||...|.|::|.
  Rat     4 KGPR------AEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQ 62

  Fly   101 FVNSLNAHGANSVWEHHLLDGSTNSTGGKHVPRWRKPTPKDALHPTKSDFIKAKHVNLTFVLKPS 165
            .|::|.::||||:|||.|||.:...:|.      ||..|:|.:||.||:||:||:..|.||.|..
  Rat    63 MVHTLASNGANSIWEHSLLDPAQVQSGR------RKANPQDKVHPIKSEFIRAKYQMLAFVHKLP 121

  Fly   166 LQDDDDGNGSAGCLEQELSRQLHASVRTSNLETSLRFLVQGADPNYYHEDKLSTPLHMAAKFGQA 230
            .:|||      |...::||:|||:||||.||||.||.|..||..|::|.:|.:||||:|||.||.
  Rat   122 CRDDD------GVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQT 180

  Fly   231 SQIEMLLIYGADVNALDGNGMTPLELARANNHNTIAERLLDAMYDVTDRIITFLGGKKPDHASGR 295
            .|.|:|::||||..:.|.||.||::.||...|:.:||||::..|::|||:..:|.|:||||.:|
  Rat   181 LQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNG- 244

  Fly   296 HMIIPD----------ANGADISEQLKIARGKLQLVPNKMFEELVMDLYDEVDRRECEAIW---- 346
            |.|||.          :...|:||..|.|:.|||.:.|::||||.||:||||||||.:|:|
  Rat   245 HYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQ 309

  Fly   347 STSTLNADHATVPFLPANPFLSATRNQGRQKLARFNRAEFTGLLTDVLVDAMRRQNMANL-RPMD 410
            :.|||..:.:.|||||.||..|||||||||||||||..||..|:.|:|.:|.|||...:| .|.|
  Rat   310 NHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTD 374

  Fly   411 APVAGHQSLQSLPYANNSMLLGSFEQGGHDPNLSDDEPIYDPVASDDDYAPVPPMAQQAIVHTPP 475
                                  :.|....:.:..||:..||.||||:|....|..:..|..:...
  Rat   375 ----------------------NLELSARNQSDLDDQHDYDSVASDEDTDQEPLPSAGATRNNRA 417

  Fly   476 RSANSHN---------EMETLRKQLNDYKSEINQLKNVVQMLSSENTQLKSKFS--SASNNSVYD 529
            ||.:|.:         |...|:|.|...::::.||..|...||.|..:|:.:..  .|.|..:..
  Rat   418 RSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRKLQREIHKLQAENLQLRQ 482

  Fly   530 EPLRIDL-SLSSPDTEH--------------------EPLSL--PEGGTANGESGS------SND 565
            .|..:.: ||.|...||                    ||.|.  |.||....|..:      |.:
  Rat   483 PPGPVPVPSLPSERAEHTLMGPGGSTHRRDRQAFSMYEPGSALKPFGGAPGDELATRLQPFHSTE 547

  Fly   566 SSNQS--TIKRPASMYERR---------------LVPNVAKGN--------------TDIRNTTS 599
            ..:.:  ::..||.:|..|               |:.:..:|:              :|..||.|
  Rat   548 LEDDAIYSVHVPAGLYRIRKGVSASSVTFTPSSPLLSSSQEGSRHASKLSRHGSGAESDYENTQS 612

  Fly   600 ------------------------MYQMAGD---GKPFGEEVKVRSDLVTRRLKELIRAMQPVPE 637
                                    :..:.||   |.|..|:|.::::.||:.::||:||.|....
  Rat   613 GEPLLGLEGKRFLELSKEDELHAELESLDGDPDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKH 677

  Fly   638 DQKQSIAPHGELIRSAVTDLIALYANLPPNASDPSRETLKLLTRQNILIQHECENLQKAIEADDK 702
            |   |..|..|.|..|||::.:|:...|  |.:|.|.:|:||......:|.||   :|.:..:..
  Rat   678 D---SFVPCSEKIHLAVTEMASLFPKRP--ALEPVRSSLRLLNASAYRLQSEC---RKTVPPEPG 734

  Fly   703 QAI--QKNTLEVRDCAFHIASAIKTLV 727
            ..:  |..|.:|..||:.||.|.|.||
  Rat   735 APVDFQLLTQQVIQCAYDIAKAAKQLV 761

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Git	NP_610599.3	ArfGap	48..164	CDD:279720	64/115 (56%)
		Ank_2	187..279	CDD:289560	48/91 (53%)
		ANK	<187..270	CDD:238125	45/82 (55%)
		ANK repeat	187..213	CDD:293786	16/25 (64%)
		ANK repeat	215..247	CDD:293786	17/31 (55%)
		ANK repeat	249..279	CDD:293786	13/29 (45%)
		GIT_SHD	314..342	CDD:285690	18/27 (67%)
		GIT	374..404	CDD:128828	18/29 (62%)
		GIT1_C	609..727	CDD:289013	39/119 (33%)
Git1	NP_114002.1	Interaction with gamma-tubulin and localization to the centrosome. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	1..124		69/131 (53%)
		ArfGap	3..124	CDD:214518	69/131 (53%)
		ANK	<123..220	CDD:238125	52/102 (51%)
		ANK 1	132..161		18/28 (64%)
		Ank_2	137..229	CDD:289560	48/91 (53%)
		ANK repeat	137..165	CDD:293786	17/27 (63%)
		ANK 2	166..195		17/28 (61%)
		ANK repeat	167..197	CDD:293786	16/29 (55%)
		ANK 3	199..228		12/28 (43%)
		Interaction with PCLO. /evidence=ECO:0000269\|PubMed:12473661	245..374		65/128 (51%)
		Interaction with PTK2/FAK1. /evidence=ECO:0000269\|PubMed:10938112	253..424		77/192 (40%)
		Interaction with ARHGEF7. /evidence=ECO:0000269\|PubMed:10938112	254..376		62/143 (43%)
		GIT_SHD	<281..301	CDD:285690	14/19 (74%)
		GIT_SHD	337..365	CDD:285690	17/27 (63%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	363..425		20/83 (24%)
		Interaction with NCK2 and GRIN3A. /evidence=ECO:0000269\|PubMed:17310244, ECO:0000269\|PubMed:24297929	375..596		48/220 (22%)
		Required for localization at synapses. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	375..596		48/220 (22%)
		GIT_CC	418..482	CDD:293167	16/63 (25%)
		Interaction with MAPK1. /evidence=ECO:0000250\|UniProtKB:Q68FF6	420..475		12/54 (22%)
		Interaction with IKBKG. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	429..629		37/199 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	574..615		6/40 (15%)
		Interaction with PXN and TGFB1I1. /evidence=ECO:0000269\|PubMed:10938112, ECO:0000269\|PubMed:12153727	646..770		42/124 (34%)
		GIT1_C	649..763	CDD:289013	41/121 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	140	1.000	Domainoid score	I4639
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	450	1.000	Inparanoid score	I1563
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D175450at33208
OrthoFinder	1	1.000	-	-		FOG0003139
OrthoInspector	1	1.000	-	-		otm45726
orthoMCL	1	0.900	-	-		OOG6_104890
Panther	1	1.100	-	-	LDO	PTHR46097
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X2107
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	10.970