DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Git and Git1

DIOPT Version :9

Sequence 1:	NP_610599.3	Gene:	Git / 36122	FlyBaseID:	FBgn0033539	Length:	731	Species:	Drosophila melanogaster
Sequence 2:	NP_001004144.1	Gene:	Git1 / 216963	MGIID:	1927140	Length:	770	Species:	Mus musculus

Alignment Length:	809	Identity:	292/809 - (36%)
Similarity:	406/809 - (50%)	Gaps:	168/809 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    36 KMPRGKSRLQTEVCGDCGAGDPSWASINRGILLCADCCSVHRSLGRHISIVKSLRQGNWEPSVLN 100
            |.||      .|||.||.|.||.||||:||:|:|.:||||||||||||||||.||...|.|::|.
Mouse     4 KGPR------AEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQ 62

  Fly   101 FVNSLNAHGANSVWEHHLLDGSTNSTGGKHVPRWRKPTPKDALHPTKSDFIKAKHVNLTFVLKPS 165
            .|::|.::||||:|||.|||.:...:|.      ||..|:|.:||.||:||:||:..|.||.|..
Mouse    63 MVHTLASNGANSIWEHSLLDPAQVQSGR------RKANPQDKVHPIKSEFIRAKYQMLAFVHKLP 121

  Fly   166 LQDDDDGNGSAGCLEQELSRQLHASVRTSNLETSLRFLVQGADPNYYHEDKLSTPLHMAAKFGQA 230
            .:|||      |...::||:|||:||||.||||.||.|..||..|::|.:|.:||||:|||.||.
Mouse   122 CRDDD------GVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQT 180

  Fly   231 SQIEMLLIYGADVNALDGNGMTPLELARANNHNTIAERLLDAMYDVTDRIITFLGGKKPDHASGR 295
            .|.|:|::||||..:.|.||.||::.||...|:.:||||::..|::|||:..:|.|:||||.:|
Mouse   181 LQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNG- 244

  Fly   296 HMIIPD----------ANGADISEQLKIARGKLQLVPNKMFEELVMDLYDEVDRRECEAIW---- 346
            |.|||.          :...|:||..|.|:.|||.:.|::||||.||:||||||||.:|:|
Mouse   245 HYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQ 309

  Fly   347 STSTLNADHATVPFLPANPFLSATRNQGRQKLARFNRAEFTGLLTDVLVDAMRRQNMANL-RPMD 410
            :.|||..:.:.|||||.||..|||||||||||||||..||..|:.|:|.:|.|||...:| .|.|
Mouse   310 NHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTD 374

  Fly   411 APVAGHQSLQSLPYANNSMLLGSFEQGGHDPNLSDDEPIYDPVASDDDYAPVPPMAQQAIVHTPP 475
                                  :.|......:..||:..||.||||:|....|..:..|..:...
Mouse   375 ----------------------NLELSARSQSELDDQHDYDSVASDEDTDQEPLPSAGATRNNRA 417

  Fly   476 RS-----------------------ANSHNEMETLRKQLNDYKSEINQLKNVVQMLSSENTQLKS 517
            ||                       |.|..:::.|.|..:....|:.:|:..:..|.:||.||:.
Mouse   418 RSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQLRQ 482

  Fly   518 KFSSASNNSVYDEPLRIDLSLSSPD-----------TEHEPLSL--PEGGT-------------- 555
            ........|:..|  |.:.:|..|.           :.:||.|.  |.|||
Mouse   483 PPGPVPPPSLPSE--RAEHTLMGPGGSTHRRDRQAFSMYEPGSALKPFGGTPGDELATRLQPFHS 545

  Fly   556 --------------------ANGESGSS---NDSSNQSTIKRPASMYERRLVPNVAKGNTDIRNT 597
                                ..|.|.||   ..||...:..:..|.:..:|..:.:..::|..||
Mouse   546 TELEDDAIYSVHVPAGLYRIRKGVSASSVPFTPSSPLLSCSQEGSRHASKLSRHGSGADSDYENT 610

  Fly   598 TS------------------------MYQMAGD---GKPFGEEVKVRSDLVTRRLKELIRAMQPV 635
            .|                        :..:.||   |.|..|:|.::::.||:.::||:||.|..
Mouse   611 QSGDPLLGLEGKRFLELSKEDELHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEF 675

  Fly   636 PEDQKQSIAPHGELIRSAVTDLIALYANLPPNASDPSRETLKLLTRQNILIQHECENLQKAIEAD 700
            ..|   |..|..|.|..|||::.:|:...|  |.:|.|.:|:||......:|.||   :|.:..:
Mouse   676 KHD---SFVPCSEKIHLAVTEMASLFPKRP--ALEPVRSSLRLLNASAYRLQSEC---RKTVPPE 732

  Fly   701 DKQAI--QKNTLEVRDCAFHIASAIKTLV 727
            ....:  |..|.:|..||:.||.|.|.||
Mouse   733 PGAPVDFQLLTQQVIQCAYDIAKAAKQLV 761

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Git	NP_610599.3	ArfGap	48..164	CDD:279720	64/115 (56%)
		Ank_2	187..279	CDD:289560	48/91 (53%)
		ANK	<187..270	CDD:238125	45/82 (55%)
		ANK repeat	187..213	CDD:293786	16/25 (64%)
		ANK repeat	215..247	CDD:293786	17/31 (55%)
		ANK repeat	249..279	CDD:293786	13/29 (45%)
		GIT_SHD	314..342	CDD:285690	18/27 (67%)
		GIT	374..404	CDD:128828	18/29 (62%)
		GIT1_C	609..727	CDD:289013	39/119 (33%)
Git1	NP_001004144.1	Interaction with gamma-tubulin and localization to the centrosome. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	1..124		69/131 (53%)
		ArfGap_GIT1	1..111	CDD:350071	64/118 (54%)
		ANK 1	132..161		18/28 (64%)
		Ank_2	137..229	CDD:372319	48/91 (53%)
		ANK repeat	137..165	CDD:293786	17/27 (63%)
		ANK 2	166..195		17/28 (61%)
		ANK repeat	167..197	CDD:293786	16/29 (55%)
		ANK 3	199..228		12/28 (43%)
		Interaction with PCLO. /evidence=ECO:0000250\|UniProtKB:Q9Z272	245..374		65/128 (51%)
		Interaction with PTK2/FAK1. /evidence=ECO:0000250\|UniProtKB:Q9Z272	253..424		76/192 (40%)
		Interaction with ARHGEF7. /evidence=ECO:0000250\|UniProtKB:Q9Z272	254..376		62/143 (43%)
		GIT_SHD	<281..301	CDD:369921	14/19 (74%)
		GIT_SHD	337..365	CDD:369921	17/27 (63%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	363..425		19/83 (23%)
		Interaction with NCK2 and GRIN3A. /evidence=ECO:0000250\|UniProtKB:Q9Z272	375..596		44/222 (20%)
		Required for localization at synapses. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	375..596		44/222 (20%)
		GIT_CC	418..482	CDD:374629	13/63 (21%)
		Interaction with MAPK1. /evidence=ECO:0000269\|PubMed:15923189	420..475		7/54 (13%)
		Interaction with IKBKG. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	429..629		35/201 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	578..615		8/36 (22%)
		Interaction with PXN and TGFB1I1. /evidence=ECO:0000250\|UniProtKB:Q9Z272	646..770		42/124 (34%)
		GIT1_C	649..763	CDD:371961	41/121 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	140	1.000	Domainoid score	I4741
eggNOG	1	0.900	-	-		E1_COG5347
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	416	1.000	Inparanoid score	I1803
Isobase	1	0.950	-	0	Normalized mean entropy	S3377
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D175450at33208
OrthoFinder	1	1.000	-	-		FOG0003139
OrthoInspector	1	1.000	-	-		otm43651
orthoMCL	1	0.900	-	-		OOG6_104890
Panther	1	1.100	-	-	LDO	PTHR46097
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3769
SonicParanoid	1	1.000	-	-		X2107
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.810