DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment KCNQ and Kcnq1

DIOPT Version :9

Sequence 1:	NP_788300.3	Gene:	KCNQ / 36071	FlyBaseID:	FBgn0033494	Length:	993	Species:	Drosophila melanogaster
Sequence 2:	NP_114462.2	Gene:	Kcnq1 / 84020	RGDID:	621503	Length:	669	Species:	Rattus norvegicus

Alignment Length:	703	Identity:	266/703 - (37%)
Similarity:	364/703 - (51%)	Gaps:	188/703 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    25 PNSERILQPRMSLLGKPLNYNRGTRRDVRYR-RLQSRLYNFLERPRGLHAIFYHVMVFLMVFTCL 88
            |.....|.||:|:.        .|||.:..| .:|.|:|||||||.|.....||..|||:|..||
  Rat    81 PRPRVSLDPRVSIY--------STRRPLLARTHIQGRVYNFLERPTGWKCFVYHFTVFLIVLVCL 137

  Fly    89 ALSVFSTIKEYEEDAVYILFRMEILVVIWFTMEFGARLWSSGCRSRYQGCLGRLKFVKRPFCIID 153
            ..||.|||::|...|...||.|||::|::|..|:..||||:||||:|.|..|||:|.::|..|||
  Rat   138 IFSVLSTIEQYAALATGTLFWMEIVLVVFFGTEYVVRLWSAGCRSKYVGIWGRLRFARKPISIID 202

  Fly   154 IVTILASIVVLGMGTSGQVFATSALRGLRFFQILRMVRMDRRGGTWKLLGSVVYAHRQELITTMY 218
            ::.::||:|||.:|:.||||||||:||:||.|||||:.:||:||||:||||||:.||||||||:|
  Rat   203 LIVVVASMVVLCVGSKGQVFATSAIRGIRFLQILRMLHVDRQGGTWRLLGSVVFIHRQELITTLY 267

  Fly   219 IGFLGLIFASFLVYMWEKD-VND----KFSNFAQALWWGVITLCTVGYGDMVPITWQGKLIASCC 278
            ||||||||:|:.||:.||| ||:    :|.::|.||||||:|:.|:||||.||.||.||.||||.
  Rat   268 IGFLGLIFSSYFVYLAEKDAVNESGRIEFGSYADALWWGVVTVTTIGYGDKVPQTWVGKTIASCF 332

  Fly   279 ALLGISFFALPAGILGSGFALKVQQQQRQKHMIRRRQPAATLIQAVWRCYAADEHSVSVATWNIH 343
            ::..|||||||||||||||||||||:|||||..|:...||:|||..||||||:....|  ||.|:
  Rat   333 SVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAASLIQTAWRCYAAENPDSS--TWKIY 395

  Fly   344 RVALPSPPASRASSSFKHNTSFVARLPTIRRHKSQTIQTPGGGDGGGVSKPPGSSRASTRYTRTI 408
                                   .|.|.    :|.|:.:|.       .||..|.....:..:..
  Rat   396 -----------------------VRKPA----RSHTLLSPS-------PKPKKSVMVKKKKFKLD 426

  Fly   409 RDINASVENLEVVQNGKSMNPSFSEDSVAETTCLKNIKNSDASQPATLANCKLSSSAGSLAQFPD 473
            :|            ||  ::|.....:|...||
  Rat   427 KD------------NG--LSPGEKIFNVPHITC-------------------------------- 445

  Fly   474 RNRDRDQDRR-GHGEGEGDQAEEQAKAGGSRRNLT-IPVVLYGFLHGNFLGSTLSLRNPRVAPAN 536
               |..:||| .|...:|..:       ..|::.| :.|....||..|..             |.
  Rat   446 ---DPPEDRRPDHFSIDGYDS-------SVRKSPTLLEVSTPHFLRTNSF-------------AE 487

  Fly   537 DRDLEAGRDEDEVHKDTCQRSNTLPLPVKPDSPSGSPGSGRTGRFFAAASHFLETGFSSPNPIID 601
            |.|||               ..||..|:                     :|
  Rat   488 DLDLE---------------GETLLTPI---------------------TH-------------- 502

  Fly   602 VANEEDEPRCTQLTNRHKTAIRFIRKLKYFVARRKFKEALKPYDVKDVMEQYAAGHVDLLGRVKM 666
                     .:||.:.|:..|:.||:::||||::||::|.|||||:||:|||:.||::|:.|:|.
  Rat   503 ---------VSQLRDHHRATIKVIRRMQYFVAKKKFQQARKPYDVRDVIEQYSQGHLNLMVRIKE 558

  Fly   667 LHLRLDQILGKQ------GSKAKDVYASKISLASRVVKVERQVADIEEKLDIL 713
            |..||||.:||.      ..|:||  ....::.:|:.:||.:|..::::|.|:
  Rat   559 LQRRLDQSIGKPSLFIPISEKSKD--RGSNTIGARLNRVEDKVTQLDQRLVII 609

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
KCNQ	NP_788300.3	Ion_trans	100..305	CDD:278921	132/209 (63%)
		Ion_trans_2	221..295	CDD:285168	49/78 (63%)
		KCNQ_channel	<619..719	CDD:281513	41/101 (41%)
Kcnq1	NP_114462.2	Ion_trans	123..359	CDD:395416	146/235 (62%)
		Selectivity filter. /evidence=ECO:0000250	312..317		3/4 (75%)
		Interaction with CALM. /evidence=ECO:0000250\|UniProtKB:P51787	370..382		7/11 (64%)
		KCNQ_channel	<511..617	CDD:397540	41/101 (41%)
		Interaction with CALM, calcium-dependent. /evidence=ECO:0000250\|UniProtKB:P51787	515..529		6/13 (46%)
		Interaction with KCNE1 C-terminus. /evidence=ECO:0000250\|UniProtKB:P51787	535..572		21/36 (58%)
		Interaction with AKAP9. /evidence=ECO:0000250\|UniProtKB:P51787	588..616		6/22 (27%)
		C-terminal assembly domain. /evidence=ECO:0000250\|UniProtKB:P51787	589..620		6/21 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1168835at2759
OrthoFinder	1	1.000	-	-		FOG0003178
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	7	6.780