DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment KCNQ and Kcnb1

DIOPT Version :9

Sequence 1:	NP_788300.3	Gene:	KCNQ / 36071	FlyBaseID:	FBgn0033494	Length:	993	Species:	Drosophila melanogaster
Sequence 2:	NP_037318.2	Gene:	Kcnb1 / 25736	RGDID:	2954	Length:	857	Species:	Rattus norvegicus

Alignment Length:	764	Identity:	166/764 - (21%)
Similarity:	280/764 - (36%)	Gaps:	216/764 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    58 QSRLYNFLERPRGLHA--IFYHVMVFLMVFTCLALSVFSTIKEYEEDAVY-------ILFRMEIL 113
            :.:|::.||:|....|  |...:.:..:|.:.:|||: :|:.|.:....:       .|..:|.:
  Rat   172 RKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSL-NTLPELQSLDEFGQSTDNPQLAHVEAV 235

  Fly   114 VVIWFTMEFGARLWSSGCRSRYQGCLGRLKFVKRPFCIIDIVTILASIVVLGMGTSG-------- 170
            .:.|||||:..|..||.         .:.||.|.|...||::.||...|.:.:..|.
  Rat   236 CIAWFTMEYLLRFLSSP---------KKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQN 291

  Fly   171 -----QVFATSALRGLRFFQILRMVRMDRRGGTWKLLGSVVYAHRQELITTMYIGFLGLIFASFL 230
                 |:|        |..:|||::::.|.....:.||..:.....||...:....:|::..|.|
  Rat   292 VRRVVQIF--------RIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSL 348

  Fly   231 VYMWEKDVND-KFSNFAQALWWGVITLCTVGYGDMVPITWQGKLIASCCALLGISFFALPAGILG 294
            |:..|||.:| ||.:...:.||..||:.||||||:.|.|..||::...|.:.|:...|||..|:.
  Rat   349 VFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIV 413

  Fly   295 SGFALKVQQQQRQKHMIRRRQPA------ATLI-----------------------QAVWRCYAA 330
            :.|:...::|:||:..|:||:..      .:::                       :::.:....
  Rat   414 NNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGESIAKKDKV 478

  Fly   331 DEHSVSVATWNIHRVALP--------------SPPASRASSSFKHNTSFVARLPTIRRHKSQTIQ 381
            .::.:|...|...:.||.              ||..:|:|||.:|..  |.:|..:....::|..
  Rat   479 QDNHLSPNKWKWTKRALSETSSSKSFETKEQGSPEKARSSSSPQHLN--VQQLEDMYSKMAKTQS 541

  Fly   382 TP--GGGDGGGVSKPPGSSRASTRYTRTIRDINASVENLEVVQNGKSMNPSFSEDSVAETTCLKN 444
            .|  ...:....||||                    |.||:     |..||......|.|..:.:
  Rat   542 QPILNTKEMAPQSKPP--------------------EELEM-----SSMPSPVAPLPARTEGVID 581

  Fly   445 IKNSDASQPATLANCKLSSSAGSLAQFPDRNRDRDQDRRGHGEGEGDQAEEQAKAGGSRRNLTIP 509
            :::..:          :.|.......||:..|          ......|...:|||.|    |.|
  Rat   582 MRSMSS----------IDSFISCATDFPEATR----------FSHSPLASLSSKAGSS----TAP 622

  Fly   510 VVLY-GFLHGNFLGSTLSLRNPRVAPANDRD---LEAGRDEDEVHKDTCQRSNTL------PLPV 564
            .|.: |.|..:  |..|:..||  .|...|.   :|:.|...:.:.....|:..:      |.|:
  Rat   623 EVGWRGALGAS--GGRLTETNP--IPETSRSGFFVESPRSSMKTNNPLKLRALKVNFVEGDPTPL 683

  Fly   565 KPD-SPSGSPGSGRTGRFFAAA----SHFLETGFSSP-NPIIDVANEEDEPRCTQLTNRHKTAIR 623
            .|. .....|...|.|...|.|    :..|:....|| :.|...|:....||..:   :| |||.
  Rat   684 LPSLGLYHDPLRNRGGAAAAVAGLECASLLDKPVLSPESSIYTTASARTPPRSPE---KH-TAIA 744

  Fly   624 FIRKLKYFVARRKFKEALKPYDVKDVMEQYAAGHVDLLGRVKMLHLRLDQILGKQGSKAKDVYAS 688
            |           .|:..:..|...|..::         |::         :.....|..|.::.|
  Rat   745 F-----------NFEAGVHHYIDTDTDDE---------GQL---------LYSVDSSPPKSLHGS 780

  Fly   689 ---KISLASRVVKVERQVADIEEKLDILIKAYMEDRDRFLALPLPAKPK 734
               |.|..:|.                       :::.|.:.|||..||
  Rat   781 TSPKFSTGART-----------------------EKNHFESSPLPTSPK 806

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
KCNQ	NP_788300.3	Ion_trans	100..305	CDD:278921	62/225 (28%)
		Ion_trans_2	221..295	CDD:285168	30/74 (41%)
		KCNQ_channel	<619..719	CDD:281513	14/102 (14%)
Kcnb1	NP_037318.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..21
		BTB_POZ_KCNB2	15..141	CDD:349719
		Self-association. /evidence=ECO:0000269\|PubMed:18463252, ECO:0000269\|PubMed:19690160	59..75
		Ion_trans	188..424	CDD:395416	69/253 (27%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:P63142	377..382		4/4 (100%)
		Self-association. /evidence=ECO:0000269\|PubMed:18463252, ECO:0000269\|PubMed:19690160	448..481		0/32 (0%)
		Kv2channel	467..678	CDD:397541	50/265 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	476..524		10/47 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	540..569		11/53 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	610..658		17/55 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	770..802		8/54 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	818..857
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810