DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pfk and PFK2

DIOPT Version :9

Sequence 1:	NP_001246234.1	Gene:	Pfk / 36060	FlyBaseID:	FBgn0003071	Length:	950	Species:	Drosophila melanogaster
Sequence 2:	NP_013932.1	Gene:	PFK2 / 855245	SGDID:	S000004818	Length:	959	Species:	Saccharomyces cerevisiae

Alignment Length:	830	Identity:	339/830 - (40%)
Similarity:	493/830 - (59%)	Gaps:	56/830 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly   134 SEKNAVKKKKSAP-----KRKCG-----KSVDELRKCLRTMQDVIDFVHPV-KPFKDKGLAVFTS 187
            |.||||..|.:.|     ..:.|     .|..:|...::|.........|: :|  .|.:||.||
Yeast   142 STKNAVSTKPTPPPAPEASAESGLSSKVHSYTDLAYRMKTTDTYPSLPKPLNRP--QKAIAVMTS 204

  Fly   188 GGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGG-DCIQEANWASVSSIIHRGGTIIGSA 251
            |||:.|||:.|||.||.||:.||:.:.:.|||:|:|.|| :.|:|.:|..|......|||.||:|
Yeast   205 GGDAPGMNSNVRAIVRSAIFKGCRAFVVMEGYEGLVRGGPEYIKEFHWEDVRGWSAEGGTNIGTA 269

  Fly   252 RCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTITTEQQEK 316
            ||.:|::|:|||..|.:||:.|:..|:|.|||||||||:|||.||.||::||:|...|:.||.|:
Yeast   270 RCMEFKKREGRLLGAQHLIEAGVDALIVCGGDGSLTGADLFRSEWPSLIEELLKTNRISNEQYER 334

  Fly   317 FNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGRHCGYLSV 381
            ...|:|.|.|||||||...||.|||..:||.||.:|||.:.:||.||.|.|::|||||:||:|::
Yeast   335 MKHLNICGTVGSIDNDMSTTDATIGAYSALDRICKAIDYVEATANSHSRAFVVEVMGRNCGWLAL 399

  Fly   382 VAGIISEADYVFLPESP-PQADWPDRLVLKLEQERSAGQRLNIVIVAEGAMDREGHPITAEDVKK 445
            :|||.:.|||:|:||.| ..::|.|::...:.:.||.|:|..||:|||||:..:..||:..||.|
Yeast   400 LAGIATSADYIFIPEKPATSSEWQDQMCDIVSKHRSRGKRTTIVVVAEGAIAADLTPISPSDVHK 464

  Fly   446 VIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDGNQAVRV 510
            |:.:||..|.|||.||||||||...|:|||||...|.||..|::|:|.|:...:|:::.|:.||.
Yeast   465 VLVDRLGLDTRITTLGHVQRGGTAVAYDRILATLQGLEAVNAVLESTPDTPSPLIAVNENKIVRK 529

  Fly   511 PLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRL-----KPPKENFDADGKGIE 570
            ||||.|:.|:|||:|:..|.:..|:.||...|..:|..:..:...     |.||:         :
Yeast   530 PLMESVKLTKAVAEAIQAKDFKRAMSLRDTEFIEHLNNFMAINSADHNEPKLPKD---------K 585

  Fly   571 GYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIA-GNVRELGWSDVSGWVGQG 634
            ..::|::::||||.|:|:||.|.....:.:|...|.|.:|..||.. .:||.|.|.|:.||..:|
Yeast   586 RLKIAIVNVGAPAGGINSAVYSMATYCMSQGHRPYAIYNGWSGLARHESVRSLNWKDMLGWQSRG 650

  Fly   635 GAYLGTKRTLP-EGKFKEIAARLKEFKIQGLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPS 698
            |:.:||.|..| |.....||...::::..||:|:||||::.:..|:...|::||.|.||:|:||:
Yeast   651 GSEIGTNRVTPEEADLGMIAYYFQKYEFDGLIIVGGFEAFESLHQLERARESYPAFRIPMVLIPA 715

  Fly   699 TISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADA 763
            |:||||||||:|||.||.||.:.|.||.::|||..|:.|.||::..||..|||||.|.||.||..
Yeast   716 TLSNNVPGTEYSLGSDTALNALMEYCDVVKQSASSTRGRAFVVDCQGGNSGYLATYASLAVGAQV 780

  Fly   764 AYIYEEKFSIKDLQQDVYHMASKMAEGVSRG----LILRNEKASENYSTDFIYRLYSEEGKGLFT 824
            :|:.||..|::.|.:|:.::|....:...||    |||::..||:..|...:..:.:.|..|.|.
Yeast   781 SYVPEEGISLEQLSEDIEYLAQSFEKAEGRGRFGKLILKSTNASKALSATKLAEVITAEADGRFD 845

  Fly   825 CRMNILGHMQQGGSPTPFDRNMGTKMAAKCVDWL-----------AAQIKANIDANGVVNCKSPD 878
            .:....||:||||.|:|.||...|:||.|.|.::           ||:...|.|...:     .|
Yeast   846 AKPAYPGHVQQGGLPSPIDRTRATRMAIKAVGFIKDNQAAIAEARAAEENFNADDKTI-----SD 905

  Fly   879 TATLLGIVSRQYRFSPLVDLI-AETNFDQRIPKKQWWLRLRPLLRILAKH 927
            ||.::|:......::.:..|. .||....|:||...|    ...|::|.|
Yeast   906 TAAVVGVKGSHVVYNSIRQLYDYETEVSMRMPKVIHW----QATRLIADH 951

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pfk	NP_001246234.1	PFK	178..914	CDD:294137	322/760 (42%)
Pfk	NP_001246234.1	6PF1K_euk	180..929	CDD:274152	326/773 (42%)
PFK2	NP_013932.1	Glo_EDI_BRP_like	17..140	CDD:211348
		PFK	196..947	CDD:294137	323/768 (42%)
		6PF1K_euk	197..957	CDD:274152	326/773 (42%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157342228
Domainoid	1	1.000	320	1.000	Domainoid score	I182
eggNOG	1	0.900	-	-		E1_COG0205
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	588	1.000	Inparanoid score	I139
Isobase	1	0.950	-	0	Normalized mean entropy	S685
OMA	1	1.010	-	-		QHG63571
OrthoFinder	1	1.000	-	-		FOG0000489
OrthoInspector	1	1.000	-	-		otm46579
orthoMCL	1	0.900	-	-		OOG6_100319
Panther	1	1.100	-	-	LDO	PTHR13697
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2168
SonicParanoid	1	1.000	-	-		X474
TreeFam	1	0.960	-	-
	15	14.740