DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pfk and Pfkl

DIOPT Version :9

Sequence 1:	NP_001246234.1	Gene:	Pfk / 36060	FlyBaseID:	FBgn0003071	Length:	950	Species:	Drosophila melanogaster
Sequence 2:	NP_032852.2	Gene:	Pfkl / 18641	MGIID:	97547	Length:	780	Species:	Mus musculus

Alignment Length:	764	Identity:	431/764 - (56%)
Similarity:	572/764 - (74%)	Gaps:	12/764 - (1%)

- Green bases have known domain annotations that are detailed below.

  Fly   180 KGLAVFTSGGDSQGMNAAVRACVRMAIYLGCKVYFIREGYQGMVDGGDCIQEANWASVSSIIHRG 244
            |.:.|.|||||:|||||||||..||.||:|.||:.|.|||:|:|:||:.|:.|||.|||:||..|
Mouse    16 KAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKPANWLSVSNIIQLG 80

  Fly   245 GTIIGSARCQDFRERQGRLKAANNLIQRGITNLVVIGGDGSLTGANLFRQEWSSLLDELVKNKTI 309
            |||||||||:.|..|:|||.||.||:|.|||||.||||||||||||:||.||.|||:||||...|
Mouse    81 GTIIGSARCKAFTTREGRLAAAYNLLQHGITNLCVIGGDGSLTGANIFRNEWGSLLEELVKEGKI 145

  Fly   310 TTEQQEKFNVLHIVGLVGSIDNDFCGTDMTIGTDTALHRIIEAIDAISSTAYSHQRTFIMEVMGR 374
            :....:.:..|.|.||||||||||||||||||||:|||||:|.||||::||.||||||::|||||
Mouse   146 SESTAQNYAHLTIAGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGR 210

  Fly   375 HCGYLSVVAGIISEADYVFLPESPPQADWPDRLVLKLEQERSAGQRLNIVIVAEGAMDREGHPIT 439
            |||||::|:.:.|.||::|:||:||:..|.:.:..:|.:.||.|.||||:|:||||:||.|.||:
Mouse   211 HCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRHGKPIS 275

  Fly   440 AEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRILACRMGAEATLALMEATKDSVPVVISLDG 504
            :..||.::.:||..|.|:||||||||||.||||||||:.:||.||.:||:|||.|:...|:||.|
Mouse   276 SSYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVSLSG 340

  Fly   505 NQAVRVPLMECVERTQAVAKAMAEKRWADAVKLRGRSFERNLETYKMLTRLKPPKENFDADGKGI 569
            ||:||:||||||:.|:.|.|||.|:|:.:|::|||||||.|.:.||:|...|..||.
Mouse   341 NQSVRLPLMECVQVTKDVQKAMDEERFDEAIQLRGRSFENNWKIYKLLAHQKVSKEK-------- 397

  Fly   570 EGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVEGLIAGNVRELGWSDVSGWVGQG 634
            ..:.||::::||||.||||||||.||..|..|..||.::||.|||..|.|:|:||.||:||:|:|
Mouse   398 SNFSLAILNVGAPAAGMNAAVRSAVRTGISEGHTVYIVHDGFEGLAKGQVQEVGWHDVAGWLGRG 462

  Fly   635 GAYLGTKRTLPEGKFKEIAARLKEFKIQGLLIIGGFESYHAAGQIADQRDNYPQFCIPIVVIPST 699
            |:.||||||||:...:.|...|:.:.|..||:|||||:|....|:.:.|..|.:.||.:.|||:|
Mouse   463 GSMLGTKRTLPKPHLEAIVENLRTYNIHALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPAT 527

  Fly   700 ISNNVPGTEFSLGCDTGLNEITEICDRIRQSAQGTKRRVFVIETMGGYCGYLATLAGLAGGADAA 764
            ||||||||:||||.||.:|...|.||||:|||.|||||||::||||||||||||:.|:|.|||||
Mouse   528 ISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAA 592

  Fly   765 YIYEEKFSIKDLQQDVYHMASKMAEGVSRGLILRNEKASENYSTDFIYRLYSEEGKGLFTCRMNI 829
            |::|:.|:|.||:.:|.||..||...:.|||:|||||..|:|:|:|:|.|||.||:|:|.||.|:
Mouse   593 YVFEDPFNIHDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGRGVFDCRTNV 657

  Fly   830 LGHMQQGGSPTPFDRNMGTKMAAKCVDWLAAQIKANIDANGVVNCKSPDTATLLGIVSRQYRFSP 894
            |||:||||:|||||||.|||:..|.:.|::.::: ::...|.|...:||:|.::|:..:...|||
Mouse   658 LGHLQQGGAPTPFDRNYGTKLGVKAMLWVSEKLR-DVYRKGRVFANAPDSACVIGLRKKVVAFSP 721

  Fly   895 LVDLIAETNFDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEE 943
            :.:|..||:|:.|:|::||||.||.:|::||.:..:..:   |::.|.|
Mouse   722 VTELKKETDFEHRMPREQWWLNLRLMLKMLAHYRISMAD---YVSGELE 767

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pfk	NP_001246234.1	PFK	178..914	CDD:294137	420/733 (57%)
Pfk	NP_001246234.1	6PF1K_euk	180..929	CDD:274152	428/748 (57%)
Pfkl	NP_032852.2	N-terminal catalytic PFK domain 1	2..390		234/373 (63%)
		Eukaryotic_PFK	13..773	CDD:238389	431/764 (56%)
		6PF1K_euk	16..755	CDD:274152	428/747 (57%)
		Substrate binding. /evidence=ECO:0000255\|HAMAP-Rule:MF_03184	164..166		1/1 (100%)
		Substrate binding. /evidence=ECO:0000255\|HAMAP-Rule:MF_03184	208..210		1/1 (100%)
		Substrate binding. /evidence=ECO:0000255\|HAMAP-Rule:MF_03184	298..301		2/2 (100%)
		Interdomain linker	391..400		3/16 (19%)
		C-terminal regulatory PFK domain 2	401..780		194/371 (52%)
		Fructose 2,6-bisphosphate binding, allosteric activator. /evidence=ECO:0000255\|HAMAP-Rule:MF_03184	527..531		3/3 (100%)
		Fructose 2,6-bisphosphate binding, allosteric activator. /evidence=ECO:0000255\|HAMAP-Rule:MF_03184	572..574		1/1 (100%)
		Fructose 2,6-bisphosphate binding, allosteric activator. /evidence=ECO:0000255\|HAMAP-Rule:MF_03184	660..663		1/2 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167838237
Domainoid	1	1.000	434	1.000	Domainoid score	I570
eggNOG	1	0.900	-	-		E1_COG0205
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	951	1.000	Inparanoid score	I341
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG63571
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000489
OrthoInspector	1	1.000	-	-		otm42642
orthoMCL	1	0.900	-	-		OOG6_100319
Panther	1	1.100	-	-	O	PTHR13697
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2168
SonicParanoid	1	1.000	-	-		X474
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.790