DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Uba1 and uba3

DIOPT Version :9

Sequence 1:	NP_477310.2	Gene:	Uba1 / 35998	FlyBaseID:	FBgn0023143	Length:	1191	Species:	Drosophila melanogaster
Sequence 2:	NP_998632.1	Gene:	uba3 / 406776	ZFINID:	ZDB-GENE-040426-2825	Length:	462	Species:	Danio rerio

Alignment Length:	499	Identity:	121/499 - (24%)
Similarity:	185/499 - (37%)	Gaps:	184/499 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly   596 KKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEKSNLNRQFLFRPHDVQ 660
            :..|..|...|..::|||.:||||||:..:    :|...|.|.|||.|:.|||||||||||.||.
Zfish    60 ESLQFLLDTCKILVIGAGGLGCELLKDLAL----SGFRHIHVVDMDTIDVSNLNRQFLFRPKDVG 120

  Fly   661 KPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLDGVANALDNVDARIYMDRKCI- 724
            :||:..|||.:....|..:|..:..::     :...|.|:.:...|...||:|.||.:|:...:
Zfish   121 RPKAEVAADFVNDRVPGCSVVPHFKKI-----QDLDETFYRQFHIVVCGLDSVIARRWMNGMLLS 180

  Fly   725 -----------FNRIPLVETGTLGTLGNVQVIVPFATESYSSSQD--PPEKSIPICTLKNFPNAI 776
                       .:.|||::.||.|..||.:||:|..|.....:.:  ||:.:.|:||:.:.|
Zfish   181 LLIYEDGVLDPSSIIPLIDGGTEGFKGNARVILPGMTACIDCTLELYPPQINFPMCTIASMP--- 242

  Fly   777 EHTLQWARDAFEGVFKQSAENAAQYIADPQFTERIAKLPGIQPLEILDSIKKALIDDKPKSFAHC 841
                                                :||                       .||
Zfish   243 ------------------------------------RLP-----------------------EHC 248

  Fly   842 VEWAR-LYWEDQYVNQIKQLLFNFPPDQITSSGQPFWSGPKRCP--DPLVFDVNDPMHLDFIYAA 903
            ||:.| |.|                              ||..|  |.:|.|.:||.|:.::|..
Zfish   249 VEYVRMLLW------------------------------PKEKPFGDGVVLDGDDPKHIQWVYQK 283

  Fly   904 ANLRAEVYGIEQVRNRETIAELVQKVKVPEFKPRSGVKIETNEAAAAASA--------------N 954
            :..||..:.|..|..|  :.:.|.|..:|       ....||...|||.|              |
Zfish   284 SLERAAEFNITGVTYR--LTQGVVKRIIP-------AVASTNAVIAAACATEVFKIATSAYVPLN 339

  Fly   955 N---FDDGELDQDRVDKII-----SELLKNADKSSKITPLEFEKDDDSNLH--MDFIVACSNLRA 1009
            |   |:|       ||.:.     :|..:|....|:: |.:.:....:.|.  :|::...::|:.
Zfish   340 NYLVFND-------VDGLYTYTFEAERKENCSACSQV-PQDMQFTPSAKLQEVLDYLTENASLQM 396

  Fly  1010 ANYKIPPADRHKSKLIAGKIIPAIATT------TSVLSGLAVLE 1047
            .:                   |||.||      |..|..:|.:|
Zfish   397 KS-------------------PAITTTLDGKNKTLYLQTVASIE 421

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Uba1	NP_477310.2	Ube1	194..1188	CDD:273603	121/499 (24%)
		Ube1_repeat1	199..574	CDD:238768
		E1_4HB	447..510	CDD:292809
		Ube1_repeat2	606..1144	CDD:238767	119/489 (24%)
uba3	NP_998632.1	Interaction with ube2m N-terminus. /evidence=ECO:0000250	52..69		2/8 (25%)
		ThiF	66..>240	CDD:279270	64/182 (35%)
		Uba3_RUB	70..367	CDD:238765	105/413 (25%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	156..160		0/3 (0%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	191..216		11/24 (46%)
		Interaction with nedd8. /evidence=ECO:0000250	226..228		0/1 (0%)
		Interaction with nae1. /evidence=ECO:0000250	241..247		3/67 (4%)
		Interaction with nae1. /evidence=ECO:0000250	291..294		0/2 (0%)
		Interaction with ube2m N-terminus. /evidence=ECO:0000250	330..337		0/6 (0%)
		Interaction with nedd8. /evidence=ECO:0000250	351..356		0/4 (0%)
		Interaction with ube2m core domain. /evidence=ECO:0000250	367..462		14/75 (19%)
		E2_bind	375..460	CDD:285976	12/66 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0476
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810