DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Uba1 and Uba3

DIOPT Version :9

Sequence 1:	NP_477310.2	Gene:	Uba1 / 35998	FlyBaseID:	FBgn0023143	Length:	1191	Species:	Drosophila melanogaster
Sequence 2:	NP_035796.2	Gene:	Uba3 / 22200	MGIID:	1341217	Length:	462	Species:	Mus musculus

Alignment Length:	650	Identity:	129/650 - (19%)
Similarity:	211/650 - (32%)	Gaps:	293/650 - (45%)

- Green bases have known domain annotations that are detailed below.

  Fly   596 KKFQEK------------------LADS-KWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMD 641
            |||.|:                  |.|: |..::|||.:|||||||..:    :|..||.|.|||
Mouse    42 KKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLAL----SGFRQIHVIDMD 102

  Fly   642 LIEKSNLNRQFLFRPHDVQKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLDGV 706
            .|:.|||||||||||.||.:||:..||:.:....|..||..:..::     :.|::.|:.:...:
Mouse   103 TIDVSNLNRQFLFRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKI-----QDFNDTFYRQFHII 162

  Fly   707 ANALDNVDARIYMDRKCI------------FNRIPLVETGTLGTLGNVQVIVPFATESYSSSQD- 758
            ...||::.||.:::...|            .:.:||::.||.|..||.:||:|..|.....:.:
Mouse   163 VCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLEL 227

  Fly   759 -PPEKSIPICTLKNFPNAIEHTLQWARDAFEGVFKQSAENAAQYIADPQFTERIAKLPGIQPLEI 822
             ||:.:.|:||:.:.|                                       :||
Mouse   228 YPPQVNFPMCTIASMP---------------------------------------RLP------- 246

  Fly   823 LDSIKKALIDDKPKSFAHCVEWAR-LYWEDQYVNQIKQLLFNFPPDQITSSGQPFWSGPKRCPDP 886
                            .||:|:.| |.|..:                     |||..|.     |
Mouse   247 ----------------EHCIEYVRMLQWPKE---------------------QPFGDGV-----P 269

  Fly   887 LVFDVNDPMHLDFIYAAANLRAEVYGIEQVRNRETIAELVQKVKVPEFKPRSGVKIETNEAAAAA 951
            |  |.:||.|:.:|:..:..||..|
Mouse   270 L--DGDDPEHIQWIFQKSIERASQY---------------------------------------- 292

  Fly   952 SANNFDDGELDQDRVDKIISELLKNADKSSKITPLEFEKDDDSNLHMDFIVACSNLRAANYKIPP 1016
                                                                  |:|...|::
Mouse   293 ------------------------------------------------------NIRGVTYRL-- 301

  Fly  1017 ADRHKSKLIAGKIIPAIATTTSVLSGLAVLEVIKLIVGHRDLVKFKNGFANLALPFMAFS----- 1076
                 ::.:..:||||:|:|.:|::.:...||.|:...  ..:...|        ::.|:
Mouse   302 -----TQGVVKRIIPAVASTNAVIAAVCATEVFKIATS--AYIPLNN--------YLVFNDVDGL 351

  Fly  1077 ----------EPLPAAKNTYYGKEWTLWDRFEVTGELSLQEFLNYFEENEKLK-----ITMLSQG 1126
                      |..||...        |....:.:....|||.|:|...:..|:     ||...:|
Mouse   352 YTYTFEAERKENCPACSQ--------LPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEG 408

  Fly  1127 VSMLYSFFMPKAKCSERLPLPMSEVVRRVSKRRLEPHERSLVFEICCNDVDGEDVEVPYVRYTLP 1191
            .:... :........||....:|:.::.:..                  |||:::.|..|  |.|
Mouse   409 KNRTL-YLQSVTSIEERTRPNLSKTLKELGL------------------VDGQELAVADV--TTP 452

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Uba1	NP_477310.2	Ube1	194..1188	CDD:273603	127/645 (20%)
		Ube1_repeat1	199..574	CDD:238768
		E1_4HB	447..510	CDD:292809
		Ube1_repeat2	606..1144	CDD:238767	114/572 (20%)
Uba3	NP_035796.2	Interaction with UBE2M N-terminus. /evidence=ECO:0000250	53..70		2/16 (13%)
		ThiF	67..366	CDD:279270	102/508 (20%)
		Uba3_RUB	71..368	CDD:238765	103/506 (20%)
		Interaction with UBE2M N-terminus. /evidence=ECO:0000250	157..161		0/3 (0%)
		Interaction with UBE2M N-terminus. /evidence=ECO:0000250	192..217		10/24 (42%)
		Interaction with NEDD8. /evidence=ECO:0000250	227..229		0/1 (0%)
		Interaction with NAE1. /evidence=ECO:0000250	242..248		3/67 (4%)
		Interaction with NAE1. /evidence=ECO:0000250	292..295		2/96 (2%)
		Interaction with UBE2M N-terminus. /evidence=ECO:0000250	331..338		0/8 (0%)
		Interaction with NEDD8. /evidence=ECO:0000250	352..357		0/4 (0%)
		Interaction with UBE2M core domain. /evidence=ECO:0000250	368..462		19/113 (17%)
		E2_bind	376..461	CDD:285976	18/97 (19%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0476
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810