Protein Alignment Mad1 and MAD1

DIOPT Version :10

• Sequence 1: NP_610477.2
• Gene: Mad1 / 35954
• FlyBaseID: FBgn0026326
• Length: 730
• Species: Drosophila melanogaster
• Sequence 2: NP_011429.3
• Gene: MAD1 / 852794
• SGDID: S000003054
• Length: 749
• Species: Saccharomyces cerevisiae
• Alignment Length: 863
• Identity: 180/863 - (20%)
• Similarity: 324/863 - (37%)
• Gaps: 260/863 - (30%)

  Fly     3 DIRSSIDDMMDRFNDSITHSAPKKLLFNRLSASFDL----GVSPNKRRRERESPERSLNDTASSL 63
            |:|:::.......:...|   .|||.....|..:.:    |.||     ..|||..|  ....|.
Yeast     2 DVRAALQCFFSALSGRFT---GKKLGLEIYSIQYKMSNSGGSSP-----FLESPGGS--PDVGST 56

  Fly    64 NMPANDSMASLQNSKLRTELIETKAIVIQLRNEIEKKSREHKEAILLAENKST------ALKDQC 122
            |..:|..:.:|| .||.|         :|...||||...:.:..||..:.|:|      ||.| .
Yeast    57 NGQSNRQIQALQ-FKLNT---------LQNEYEIEKLQLQKQTNILEKKYKATIDELEKALND-T 110

  Fly   123 DITSKKNLELQDDLKALRKRELVLKNEASRATAEL-----------NQLRLKFD----------- 165
            ....:.|.:|:.:||:|::|.....|:..:...||           ..||.::|
Yeast   111 KYLYESNDKLEQELKSLKERSANSMNDKDKCIEELRTTLQNKDLEMETLRQQYDSKLSKVTNQCD 175

  Fly   166 ------EST----LKLQKEKYLQKEDARDVH----------------LCIN----------NELS 194
                  ||:    :|.:||...|..|.:|:.                ..||          |||:
Yeast   176 HFKLEAESSHSLLMKYEKEIKRQSVDIKDLQHQVMEKDDELSSVKASKMINSHPNYSTEEFNELT 240

  Fly   195 EYRRIAQ-----RADLELQSTR--NELERLRQL----------NEELQARASGFEQLRANHEKQT 242
            |..::.|     ..:|||.:.:  |||::|:|.          ||:||.:.|....|    |.|.
Yeast   241 EMNKMIQDQVQYTKELELANMQQANELKKLKQSQDTSTFWKLENEKLQNKLSQLHVL----ESQY 301

  Fly   243 QSLKVAN----DRIQELEFEIQSYSDWKEVVKTSRERLASVPDLLAEVEHLRSHNKHLNTLIGDK 303
            ::|::.|    .::.:.|....|..|....|..:.....:..|...:..:..|:|.::|.
Yeast   302 ENLQLENIDLKSKLTKWEIYNDSDDDDDNNVNNNDNNNNNKNDNNNDNNNDTSNNNNINN----- 361

  Fly   304 LLLEEQVYDYKTRLEREEGARAEAASLQVKLLHMEQELKEWVKVAQDHCLANTLVSPMALRSRIE 368
                    :.:|:.....              :.|:.:::| |:.:..||   :::.|..:.|::
Yeast   362 --------NNRTKNNIRN--------------NPEEIIRDW-KLTKKECL---ILTDMNDKLRLD 400

  Fly   369 QLLKEDIIHVAEKTSSASDTKHLNTTIRDLEHKCAIYLKNIEDLN-------IGLKRHKNFKERL 426
                                   |..::.|..:.|:....|.|||       :.|||..:  |..
Yeast   401 -----------------------NNNLKLLNDEMALERNQILDLNKNYENNIVNLKRLNH--ELE 440

  Fly   427 QRKLITVSKERDFYKQL-------------VENFDKDTTLSNASVADMTQDMQVRVRMEVLERTV 478
            |:|.::..:.|...:||             |||  .:|..||.:|   .:||         ...:
Yeast   441 QQKSLSFEECRLLREQLDGLYSAQNNALLEVEN--SETHASNKNV---NEDM---------NNLI 491

  Fly   479 TGYKDMCATLEREIQSLRQQ---------------ELVNEPAGEGYDSVKKELDTLRMENDRLRR 528
            ..||:....|..|::.|..|               :|.::..|..|   .:.|:.|::||..:.|
Yeast   492 DTYKNKTEDLTNELKKLNDQLLSNSNDVETQRKKRKLTSDQIGLNY---SQRLNELQLENVSVSR 553

  Fly   529 R--KEELEMEMMHRCLRGDFNMKD--FKVVHFSENPAAEAYESTKN---MMEKLQAE-IERLKRR 585
            .  |.:..::::...|.....:|:  .:::...:.|..:.....||   ::||..|: :..||:.
Yeast   554 ELSKAQTTIQLLQEKLEKLTKLKEKKIRILQLRDGPFIKDQFIKKNKLLLLEKENADLLNELKKN 618

  Fly   586 NKKLED------DNEQRLNETTSTGGMTLNFKEFNQLQAELESANGKMRKMRDCFKAAREEFRDV 644
            |..:|.      |              :||| |..|.:.|:..:|.:..:::..|.....||.||
Yeast   619 NPAVETVPISVYD--------------SLNF-ELKQFEQEVFKSNKRFSRLKQVFNNKSLEFIDV 668

  Fly   645 CYMLLGYRIDRIGANSNYRISSMFAEGPDDYLDISLNESNCLALLESP---YSHTFNPPIDQQLA 706
            ...|||:::: ...:|..:|.|.|.  |:.||...|||:...:.|::.   :....|..::.:
Yeast   669 VNSLLGFKLE-FQQDSRVKIFSCFK--PEKYLIADLNENTLKSNLDADIEGWDDLMNLWVEDR-- 728

  Fly   707 ASNFPAFFSALTLELFQK 724
             ...|.|.:.:||.|:::
Yeast   729 -GQLPCFLATITLRLWEQ 745

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad1	NP_610477.2	MAD	80..726	CDD:461677	159/782 (20%)
MAD1	NP_011429.3	MAD	83..747	CDD:461677	153/762 (20%)