Protein Alignment Mad1 and MAD1L1

DIOPT Version :10

• Sequence 1: NP_610477.2
• Gene: Mad1 / 35954
• FlyBaseID: FBgn0026326
• Length: 730
• Species: Drosophila melanogaster
• Sequence 2: NP_003541.2
• Gene: MAD1L1 / 8379
• HGNCID: 6762
• Length: 718
• Species: Homo sapiens
• Alignment Length: 740
• Identity: 188/740 - (25%)
• Similarity: 348/740 - (47%)
• Gaps: 103/740 - (13%)

  Fly    54 RSLNDTAS-----------SLNMPANDSMASLQNSKL--RTELIETKAIVIQLRNEIEKKSREHK 105
            ||||:..|           |.:.|.:..|...|:.:|  |.|.|.:|:.:||:..|..:....||
Human    15 RSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRSKSHLIQVEREKMQMELSHK 79

  Fly   106 EAILLAENKSTALKDQCDITSKKNLELQDDLKALRKRELVLKNEASRATAELNQLRLKFDESTLK 170
            .|.:..|..::......:....:|.||...::.|::||...:.:.........|.:...|.::.:
Human    80 RARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDAASKR 144

  Fly   171 LQKEKYLQKED----ARDVHLCINNELSEYR--------RIAQRADLELQSTRNELE----RLRQ 219
            |:     :|||    |.:....:...:||.:        |: :|.:.|.|..:.:|:    :.::
Human   145 LR-----EKEDSLAQAGETINALKGRISELQWSVMDQEMRV-KRLESEKQELQEQLDLQHKKCQE 203

  Fly   220 LNEELQ-ARASGFEQLRANHEKQTQSLKVANDRIQELEFEIQSYSDWKEVVKTSRERLASVPDLL 283
            .|:::| .:||  ::.||:||:|.:.|:      |:|..:.|..:    :||..:..|..:|.|.
Human   204 ANQKIQELQAS--QEARADHEQQIKDLE------QKLSLQEQDAA----IVKNMKSELVRLPRLE 256

  Fly   284 AEVEHLRSHNKHLNTLIGDKLLLEEQVYDYKTRLEREEGARAEAASLQVKLLHMEQELKEWVKVA 348
            .|::.||..:.||..:.....||:|::...:.:|.|:|..:.....|:::...:..:|:.|.::
Human   257 RELKQLREESAHLREMRETNGLLQEELEGLQRKLGRQEKMQETLVGLELENERLLAKLQSWERL- 320

  Fly   349 QDHCLANTLVSPMALRSRIEQLLKEDIIHVAEKTSSASDTKHLNTTIRDLEHKCAIYLKNIED-- 411
             |..:..::.:|..|...:.:|.:.::....:.::..|..:.|....:.|:.:    |:.:..
Human   321 -DQTMGLSIRTPEDLSRFVVELQQRELALKDKNSAVTSSARGLEKARQQLQEE----LRQVSGQL 380

  Fly   412 LNIGLKR--HKNFKERLQRKLITVSKERDFYKQLVENFDKDTTLSNAS---------VADMTQ-- 463
            |....||  |:....|||::::.::||||..:.::.::|.:.|.:..|         ..||.|
Human   381 LEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKV 445

  Fly   464 -----DMQVRVRMEVLERTVTGYKDMCATLEREIQSLRQQELVNEPAGEGYDSVKKELDTLRM-- 521
                 :|:.::...:.|  :.|.|.....||.|::.|:.|   :..|.:.:...::|.||||:
Human   446 HSHSAEMEAQLSQALEE--LGGQKQRADMLEMELKMLKSQ---SSSAEQSFLFSREEADTLRLKV 505

  Fly   522 -----ENDRLRRRKEELEMEMMHRCLRGDFNMKDFKVVHFSENPAAEAYESTKNMMEKLQAEIER 581
                 |..||...|..||.::..|.|:||::....||:|.|.||.:.|.:..:....:||||.||
Human   506 EELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECER 570

  Fly   582 LK------RRNKKLEDDNEQRLNETTSTGGMTLNFKEFNQLQAELESANGKMRKMRDCFKAAREE 640
            |:      .|...:..|.|.......|:       ||..:|:.::|||..|.:::::.|:...:|
Human   571 LRGLLRAMERGGTVPADLEAAAASLPSS-------KEVAELKKQVESAELKNQRLKEVFQTKIQE 628

  Fly   641 FRDVCYMLLGYRIDRIGANSNYRISSMFAEGPDDYLDISLN--ESNCLALLESPYSHTFNPPIDQ 703
            ||..||.|.||:|| |...:.||::|::||.|.|.|.....  ..:.:.|||:.:|||....|:.
Human   629 FRKACYTLTGYQID-ITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEV 692

  Fly   704 QL-AASNFPAFFSALTLELFQKATV 727
            .| ...:.|||.|:||||||.:.||
Human   693 HLRRQDSIPAFLSSLTLELFSRQTV 717

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mad1	NP_610477.2	MAD	80..726	CDD:461677	176/698 (25%)
MAD1L1	NP_003541.2	MAD	54..716	CDD:461677	176/698 (25%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:19010891	79..82		1/2 (50%)
		Important for interaction with IK. /evidence=ECO:0000269|PubMed:22351768	301..340		6/40 (15%)
		Necessary for interaction with NEK2. /evidence=ECO:0000269|PubMed:14978040	380..532		39/156 (25%)
		Important for interaction with IK. /evidence=ECO:0000269|PubMed:22351768	439..480		10/42 (24%)
		Necessary for interaction with MAD2L1. /evidence=ECO:0000269|PubMed:19010891	540..551		6/10 (60%)