DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pkn and pkn2a

DIOPT Version :9

Sequence 1:	NP_001097237.1	Gene:	Pkn / 35950	FlyBaseID:	FBgn0020621	Length:	1501	Species:	Drosophila melanogaster
Sequence 2:	XP_700704.3	Gene:	pkn2a / 571965	ZFINID:	ZDB-GENE-061207-42	Length:	970	Species:	Danio rerio

Alignment Length:	1412	Identity:	560/1412 - (39%)
Similarity:	697/1412 - (49%)	Gaps:	475/1412 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   113 LSHKYGFTEN--LPESCMSIRLEEIKEAIRREIRKELKIKEGAEKLREVAKDRRSLSDVAVLVKK 175
            :|.:.|...|  |.::.:..:|:||||.|:||||||||||||.|.||:|..:::.|:.|..::||
Zfish    11 VSEQLGLGHNLDLSDTMVQQKLDEIKEQIKREIRKELKIKEGVENLRKVTTEKKRLAYVDNMLKK 75

  Fly   176 SKSKLAELKSELQELESQI-------LLTSANTAVNSNGQESITACIDPNGGFLVSGAVGGLGGG 233
            |..||.||..|||||.:.|       ||......::|...||.|
Zfish    76 SNKKLEELHQELQELNAHIVVKDPEDLLLECPLTLDSPKSESRT--------------------- 119

  Fly   234 NTALEGGAPATANDKVLASLEKQLQIEMKVKTGAENMIQ--SLGIGCDKKLLAEAHQMLADSKAK 296
                     .|::.: ||:|:||..||:|||.|||||||  |.|...|:||||.|.|||.|||.|
Zfish   120 ---------CTSSSR-LAALKKQNDIELKVKQGAENMIQMYSNGSSKDRKLLATAQQMLQDSKTK 174

  Fly   297 IEFLRLRIIKVKQNREQADRLKASRQMIDEHGQTIGGNNSSQPQSLETTLEERIEELRHRLRIEA 361
            |||:|::|:|..|..|.:         .|        ||....:.:.:.|:.|:|||.|..|||.
Zfish   175 IEFIRMQILKASQTSELS---------FD--------NNDVIAKPIISPLDLRVEELCHHTRIEF 222

  Fly   362 AVVDGAKNVIRTLQTANRAPDKKALQEAHGRLSESSRKLDLLRYSLDLRRQELPADSPAAQQLKT 426
            ||.:|||||::.|.:...| :|:|..||..|.:|||:|||||:|||:.|..|||.:.|....:..
Zfish   223 AVAEGAKNVMKLLGSGKLA-EKRAHSEAQARFNESSQKLDLLKYSLEQRLNELPKNHPKCSLIME 286

  Fly   427 ELQIVQLSTSPA---PVTYTSLQSGQAGI---LGGKPYQSVSSLGRCASVTGKLEVRLLGCQDLL 485
            ||   .|.:|||   ..::.|.|:..:.:   ...||          |::||||||||:||||||
Zfish   287 EL---SLMSSPALSPRQSFISTQNHNSTVKNQYSTKP----------AALTGKLEVRLMGCQDLL 338

  Fly   486 EDVPGRSRRDKD--NNSSPGDLRS--FVKGVTSRSSSKSYSVKDETSIEIMAVIKLDNITVGQTS 546
            |.|||||:....  ...||.:.||  ..:|....:|.::.|..|:.|.||.||:||||..|||||
Zfish   339 ESVPGRSKATSVPLPGWSPSETRSSFMSRGNRRGTSVRNLSKNDDLSNEISAVLKLDNTVVGQTS 403

  Fly   547 WKQCSQQAWDQRFSIDLDRSRELEIGVYWRDWRSLCAVKVLRLEEFIDDVRHGMALQLEPQGLLF 611
            ||..|.|:|||:|:::|||||||||.|||||||||||||.||||:|:|:.||||.|.|||||.||
Zfish   404 WKPVSNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLF 468

  Fly   612 AEVKFLNPMISQKPKLRRQRMIFNRQQAKNISRAKQMNINVATWGRLLKRNAPNHVHMGSAGSGS 676
            |||.|.||:|.::.||:||:.||::||.:...||.||:|  .|||||::|..|
Zfish   469 AEVTFFNPVIERRTKLQRQKKIFSKQQGRTFLRAPQMSI--GTWGRLVRRAIP------------ 519

  Fly   677 SLTGSSPMVVGGSRDSESPISRTPSSDALVEPEPYTPGEQAQNLEFDPDAGINEHVETPGEYPDP 741
                 ||      .:|.||::                          .:.|.:.|..||....||
Zfish   520 -----SP------NNSFSPLA--------------------------AETGHSSHPGTPALRSDP 547

  Fly   742 AASGLSGMRPLSMHMQGISVLPPESPPVATGAAGRPNTLSLQMPGASKGQVIQGGRTAAPTTAPP 806
            ..:.|...|
Zfish   548 IVTKLDFDR-------------------------------------------------------- 556

  Fly   807 PPPVLKATSTTPILDQELQDALHEFDFLSDLDSRPTTLRRLLKEQKMALDAGALENLLLQQQQTE 871
                    .|||                  .:..|.::|.:|.|.|::
Zfish   557 --------DTTP------------------KNYSPVSVREVLPEDKIS----------------- 578

  Fly   872 QQALQQRQRQEQLRLQQFQEAQRQAILDLCVKQREPAEQTSPTLANQTPTFPPLASNQIMPSLNA 936
                                          .|:||....|:....|...:
Zfish   579 ------------------------------NKERERGALTAFAYRNDRNS--------------- 598

  Fly   937 RPCQSQSPNHNEFQLQPQPPHPAQKPTNPEPPSAVEQQLHRPVLILPPVVLLGGREE--DRSVPP 999
                                                            :::..|.||  :...||
Zfish   599 ------------------------------------------------IIVQSGLEEIVEHDTPP 615

  Fly  1000 SPLVEYPEDDDEYLYRGSNENLGTRLHSSHSSSAGDRFCVEVIPQLGKLYVGSSQQQYAQQSSPI 1064
            |.|                                         ||
Zfish   616 SAL-----------------------------------------QL------------------- 620

  Fly  1065 IQEPATPTIYGNSAAAGAPQFPQPAQRQEKQPPQQQPIYANQYELNVAKAAAAASVYSPSSSTTS 1129
                                                        :||.|            ||..
Zfish   621 --------------------------------------------INVQK------------STEI 629

  Fly  1130 NSNQQQQQQRRNVARGLQYRESGGLETGRAGKQPPNAGMLSMDNFRLLSVLGRGHFGKVILSQLR 1194
            ...::|..                               .|:.:|:.::||||||||||:|::..
Zfish   630 RDEEEQFH-------------------------------FSLKDFKCVAVLGRGHFGKVLLAEYT 663

  Fly  1195 SNNQYYAIKALKKGDIIARDEVESLLSEKRIFEVANAMRHPFLVNLYSCFQTEQHVCFVMEYAAG 1259
            ...:.:||||||||||:||||||||:.||||||..|::||||||||::||||:.|||||||||||
Zfish   664 ITGEMFAIKALKKGDIVARDEVESLMCEKRIFETVNSVRHPFLVNLFACFQTKDHVCFVMEYAAG 728

  Fly  1260 GDLMMHIHTDVFLEPRAVFYAACVVLGLQYLHENKIIYRDLKLDNLLLDTEGYVKIADFGLCKEG 1324
            ||||||||.|||.||||.|||||||||||:|||:||:|||||||||||||||:||||||||||||
Zfish   729 GDLMMHIHADVFSEPRATFYAACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEG 793

  Fly  1325 MGFGDRTGTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLVGESPFPGDDEEEVFDSIVND 1389
            ||:.|||.|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Zfish   794 MGYRDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLVGESPFPGDDEEEVFDSIVND 858

  Fly  1390 EVRYPRFLSLEAIAVMRRLLRKNPERRLGSSERDAEDVKKQAFFRSIVWDDLLLRKVKPPFVPTI 1454
            ||||||.||.|||::||||||:|||||||::|||||||||..|||.|.||.||.:||||||||||
Zfish   859 EVRYPRVLSTEAISIMRRLLRRNPERRLGAAERDAEDVKKHLFFRDIDWDGLLAKKVKPPFVPTI 923

  Fly  1455 NHLEDVSNFDEEFTSEKAQLTPPKEPRHLTEEEQLLFQDFSYTAEWC 1501
            ....||||||:|||||...||||:|||.|.:.||.||.||.|.|:||
Zfish   924 QCSSDVSNFDDEFTSEAPVLTPPREPRPLNQHEQDLFTDFDYIADWC 970

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkn	NP_001097237.1	HR1_PKN_1	131..194	CDD:212012	36/62 (58%)
		HR1_PKN_2	243..309	CDD:212013	39/67 (58%)
		HR1_PKN_3	340..414	CDD:212015	35/73 (48%)
		C2	530..611	CDD:301316	59/80 (74%)
		STKc_PKN	1174..1499	CDD:270741	257/324 (79%)
		S_TKc	1174..1433	CDD:214567	213/258 (83%)
pkn2a	XP_700704.3	HR1_PKN_1	29..94	CDD:212012	36/64 (56%)
		HR1	116..189	CDD:294066	43/103 (42%)
		HR1	201..274	CDD:294066	35/73 (48%)
		C2_PKN-like	387..468	CDD:176069	59/80 (74%)
		STKc_PKN	643..968	CDD:270741	257/324 (79%)
		S_TKc	643..902	CDD:214567	213/258 (83%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170583297
Domainoid	1	1.000	461	1.000	Domainoid score	I462
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2054
Inparanoid	1	1.050	966	1.000	Inparanoid score	I307
OMA	1	1.010	-	-		QHG49182
OrthoDB	1	1.010	-	-		D241576at33208
OrthoFinder	1	1.000	-	-		FOG0001458
OrthoInspector	1	1.000	-	-		otm25508
orthoMCL	1	0.900	-	-		OOG6_103771
Panther	1	1.100	-	-	O	PTHR24356
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X914
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	14	13.910