DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pkn and pkn2b

DIOPT Version :9

Sequence 1:	NP_001097237.1	Gene:	Pkn / 35950	FlyBaseID:	FBgn0020621	Length:	1501	Species:	Drosophila melanogaster
Sequence 2:	XP_009300691.1	Gene:	pkn2b / 567134	ZFINID:	ZDB-GENE-061013-393	Length:	984	Species:	Danio rerio

Alignment Length:	1432	Identity:	562/1432 - (39%)
Similarity:	724/1432 - (50%)	Gaps:	484/1432 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly    95 NYEISAEGEYIKHPVLYELSHKYGFTENL--PESCMSIRLEEIKEAIRREIRKELKIKEGAEKLR 157
            |:...|.|..:        |.:..|.:||  .::.:...|:|||:.|:||||||||||||||.||
Zfish    12 NFVNDARGPMV--------SGRLDFDQNLDFSDTMVQKNLDEIKDQIKREIRKELKIKEGAENLR 68

  Fly   158 EVAKDRRSLSDVAVLVKKSKSKLAELKSELQELESQILLTSANTAVNSNGQESITACIDPNGGFL 222
            :|..|::||:.|..::|||..|:.||..|||||.:.|::        .:.:|.:...:.|:
Zfish    69 KVTTDKKSLAYVDNMLKKSNKKVEELHQELQELNAHIVV--------KDPEEVVEYPLTPD---- 121

  Fly   223 VSGAVGGLGGGNTALEGGAPATANDKVLASLEKQLQIEMKVKTGAENMIQ--SLGIGCDKKLLAE 285
                         ..:.....:.|...||:|:||..||:|||.|||||||  |.|...|:||||.
Zfish   122 -------------TPKSETRMSTNSNRLAALKKQADIELKVKQGAENMIQMYSNGSSKDRKLLAA 173

  Fly   286 AHQMLADSKAKIEFLRLRIIKVKQNREQADRLKASRQMIDEHGQTIGGNNSSQPQSLETTLEERI 350
            |.|||.|||.||||:|::|:|..|..|           |:.....:   .:|:|  :.:.|:.||
Zfish   174 AQQMLQDSKTKIEFIRMQILKASQTSE-----------INYENNDV---TTSKP--IISPLDLRI 222

  Fly   351 EELRHRLRIEAAVVDGAKNVIRTLQTANRAPDKKALQEAHGRLSESSRKLDLLRYSLDLRRQELP 415
            |||||..|||:||.||||||::.|.| .:..:|||..||..||:|||:|||||::||:.|..|||
Zfish   223 EELRHHYRIESAVADGAKNVMKLLGT-GKVTEKKAHSEAQARLNESSQKLDLLKFSLEQRLSELP 286

  Fly   416 ADSPAAQQLKTELQIVQLSTSPAPVTYTSLQSGQAGILGGKPYQSV-------SSLGRCASVTGK 473
            .:.|....:..||.:|   .||.                ..|.||:       |::.:.|::||.
Zfish   287 KNHPKGTLIMEELAMV---ASPP----------------NSPRQSIMSTSNQYSTVAKPAALTGT 332

  Fly   474 LEVRLLGCQDLLEDVPGRSRRDKDN--NSSPGDLRS--FVKGVTSRS-SSKSYSVKDETSIEIMA 533
            |:|||:|||||||:|||||:....:  ..||.:.||  ..:|..::| ||::.|..|:.|.||.|
Zfish   333 LDVRLMGCQDLLENVPGRSKTASVSLPGWSPSEARSSFMSRGNKNKSGSSRTLSKSDDLSNEISA 397

  Fly   534 VIKLDNITVGQTSWKQCSQQAWDQRFSIDLDRSRELEIGVYWRDWRSLCAVKVLRLEEFIDDVRH 598
            |:||||..||||.||..|.|:|||:|:::|||||||||.|||||||||||||.||||:|:|:.||
Zfish   398 VLKLDNTVVGQTHWKPVSNQSWDQKFTLELDRSRELEIAVYWRDWRSLCAVKFLRLEDFLDNQRH 462

  Fly   599 GMALQLEPQGLLFAEVKFLNPMISQKPKLRRQRMIFNRQQAKNISRAKQMNINVATWGRLLKRN- 662
            ||.|.|||||.|||||.|.||:|.::|||:||:.||::||.|...||.|||||:||||||::|.
Zfish   463 GMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAI 527

  Fly   663 -------APNHVHMGSAGSGSSLTGSSPMVVGGSRDSESPISRTPSSDALVEPEPYTPGEQAQNL 720
                   :|....:|||.|..    ::||   |..|:.|    .||...:.:            |
Zfish   528 PSVNTSFSPQAADLGSAMSHE----TAPM---GHPDAHS----LPSDPTVTK------------L 569

  Fly   721 EFDPDAGINEHVETPGEYPDPAASGLSGMRPLSMHMQGISVLPPESPPVATGAAGRPNTLSLQMP 785
            :||.                                   :|.||          .:.|::.:::
Zfish   570 DFDK-----------------------------------AVTPP----------SKRNSIEVEI- 588

  Fly   786 GASKGQVIQGGRTAAPTTAPPPPPVLKATSTTPILD-QELQDALHEFDFLSDLDSRPTTLRRLLK 849
                           ..||||          ..|.| :|:||||..||||::..::|
Zfish   589 ---------------EETAPP----------DKISDGKEVQDALATFDFLNNTVAKP-------- 620

  Fly   850 EQKMALDAGALENLLLQQQQTEQQALQQRQRQEQLRLQQFQEAQRQAILDLCVKQREPAEQTSPT 914
                  |..:|       .:.||..|:..:.|.:..:::.:|.|
Zfish   621 ------DYDSL-------VEHEQPGLELTEIQRKTEIREEEEVQ--------------------- 651

  Fly   915 LANQTPTFPPLASNQIMPSLNARPCQSQSPNHNEFQLQPQPPHPAQKPTNPEPPSAVEQQLHRPV 979

Zfish   652 ----------------------------------------------------------------- 651

  Fly   980 LILPPVVLLGGREEDRSVPPSPLVEYPEDDDEYLYRGSNENLGTRLHSSHSSSAGDRFCVEVIPQ 1044

Zfish   652 ----------------------------------------------------------------- 651

  Fly  1045 LGKLYVGSSQQQYAQQSSPIIQEPATPTIYGNSAAAGAPQFPQPAQRQEKQPPQQQPIYANQYEL 1109

Zfish   652 ----------------------------------------------------------------- 651

  Fly  1110 NVAKAAAAASVYSPSSSTTSNSNQQQQQQRRNVARGLQYRESGGLETGRAGKQPPNAGMLSMDNF 1174
                                                                       .|:.:|
Zfish   652 -----------------------------------------------------------FSLSDF 657

  Fly  1175 RLLSVLGRGHFGKVILSQLRSNNQYYAIKALKKGDIIARDEVESLLSEKRIFEVANAMRHPFLVN 1239
            :.::||||||||||:|:..::..:.:||||||||||:|||||:||:.||||||..|::|||||||
Zfish   658 KCVAVLGRGHFGKVLLADYKTTGEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVN 722

  Fly  1240 LYSCFQTEQHVCFVMEYAAGGDLMMHIHTDVFLEPRAVFYAACVVLGLQYLHENKIIYRDLKLDN 1304
            |::||||::|||||||||||||||||||.|||.|.|:||||||||||||:||::||:||||||||
Zfish   723 LFACFQTKEHVCFVMEYAAGGDLMMHIHADVFSETRSVFYAACVVLGLQFLHDHKIVYRDLKLDN 787

  Fly  1305 LLLDTEGYVKIADFGLCKEGMGFGDRTGTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLV 1369
            |||||||||||||||||||||||.|||.|||||||||||||||||||||||||||||||||||||
Zfish   788 LLLDTEGYVKIADFGLCKEGMGFKDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLV 852

  Fly  1370 GESPFPGDDEEEVFDSIVNDEVRYPRFLSLEAIAVMRRLLRKNPERRLGSSERDAEDVKKQAFFR 1434
            |||||||||||||||||||||||||::||.|||::||||||:|||||||:.|||||:||:..|||
Zfish   853 GESPFPGDDEEEVFDSIVNDEVRYPKYLSTEAISIMRRLLRRNPERRLGAGERDAEEVKRHPFFR 917

  Fly  1435 SIVWDDLLLRKVKPPFVPTINHLEDVSNFDEEFTSEKAQLTPPKEPRHLTEEEQLLFQDFSYTAE 1499
            .:.|..||.:|::|||||||...|||||||:|||||...||||:|||.||..||.||.||.|.|:
Zfish   918 DMDWPGLLAKKIRPPFVPTITSREDVSNFDDEFTSEAPILTPPREPRILTLGEQDLFADFDYIAD 982

  Fly  1500 WC 1501
            ||
Zfish   983 WC 984

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkn	NP_001097237.1	HR1_PKN_1	131..194	CDD:212012	37/62 (60%)
		HR1_PKN_2	243..309	CDD:212013	39/67 (58%)
		HR1_PKN_3	340..414	CDD:212015	39/73 (53%)
		C2	530..611	CDD:301316	58/80 (73%)
		STKc_PKN	1174..1499	CDD:270741	251/324 (77%)
		S_TKc	1174..1433	CDD:214567	209/258 (81%)
pkn2b	XP_009300691.1	HR1_PKN_1	40..105	CDD:212012	37/64 (58%)
		HR1_PKN2_2	126..199	CDD:212021	40/72 (56%)
		HR1_PKN2_3	212..285	CDD:212025	40/75 (53%)
		C2_PKN-like	394..475	CDD:176069	58/80 (73%)
		STKc_PKN	657..982	CDD:270741	251/324 (77%)
		S_TKc	657..916	CDD:214567	209/258 (81%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170583298
Domainoid	1	1.000	461	1.000	Domainoid score	I462
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	966	1.000	Inparanoid score	I307
OMA	1	1.010	-	-		QHG49182
OrthoDB	1	1.010	-	-		D241576at33208
OrthoFinder	1	1.000	-	-		FOG0001458
OrthoInspector	1	1.000	-	-		otm25508
orthoMCL	1	0.900	-	-		OOG6_103771
Panther	1	1.100	-	-	O	PTHR24356
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	1	1.000	-	-		X914
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	12.030