Protein Alignment Pkn and PKN2

DIOPT Version :10

• Sequence 1: NP_001097237.1
• Gene: Pkn / 35950
• FlyBaseID: FBgn0020621
• Length: 1501
• Species: Drosophila melanogaster
• Sequence 2: NP_006247.1
• Gene: PKN2 / 5586
• HGNCID: 9406
• Length: 984
• Species: Homo sapiens
• Alignment Length: 1431
• Identity: 578/1431 - (40%)
• Similarity: 734/1431 - (51%)
• Gaps: 472/1431 - (32%)

  Fly    94 SNYEISAEGEYIKHPVLYEL---SHKYGFTENL--------PESCMSIRLEEIKEAIRREIRKEL 147
            ||.|   .||.:    |.||   |....|:||:        .::.:..:|::||:.|:|||||||
Human     3 SNPE---RGEIL----LTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKEL 60

  Fly   148 KIKEGAEKLREVAKDRRSLSDVAVLVKKSKSKLAELKSELQELESQILLTSANTAVNSNGQESIT 212
            |||||||.||:|..|::||:.|..::|||..||.||..:||||.:.|:::.         .|.||
Human    61 KIKEGAENLRKVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSD---------PEDIT 116

  Fly   213 AC----IDPNGGFLVSGAVGGLGGGNTALEGGAPATANDKVLASLEKQLQIEMKVKTGAENMIQ- 272
            .|    ..||.....|                   |:|:: |.:|:|||.||:|||.|||||||
Human   117 DCPRTPDTPNNDPRCS-------------------TSNNR-LKALQKQLDIELKVKQGAENMIQM 161

  Fly   273 -SLGIGCDKKLLAEAHQMLADSKAKIEFLRLRIIKVKQNREQA-DRLKASRQMIDEHGQTIGGNN 335
             |.|...|:||...|.|:|.|||.|||.:|::|::..|..|.| |..|
Human   162 YSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAK----------------- 209

  Fly   336 SSQPQSLETTLEERIEELRHRLRIEAAVVDGAKNVIRTLQTANRAPDKKALQEAHGRLSESSRKL 400
                 .:.:.||.|:|||||..|||.||.:|||||::.| .:.:..|:|||.||..|.:|||:||
Human   210 -----PVISPLELRMEELRHHFRIEFAVAEGAKNVMKLL-GSGKVTDRKALSEAQARFNESSQKL 268

  Fly   401 DLLRYSLDLRRQELPADSPAAQQLKTELQIVQLSTSPAPVTYTSLQSGQAGILGGKPYQSVSSLG 465
            |||:|||:.|..|:|.:.|.::.:..||.:|..|.:.:|  ..|:.|.|         ...|:|.
Human   269 DLLKYSLEQRLNEVPKNHPKSRIIIEELSLVAASPTLSP--RQSMISTQ---------NQYSTLS 322

  Fly   466 RCASVTGKLEVRLLGCQDLLEDVPGRSRRDKD--NNSSPGDLRSFVKGVTSRS---SSKSYSVKD 525
            :.|::||.|||||:||||:||:|||||:....  ...||.:.||.....||:|   ||::....|
Human   323 KPAALTGTLEVRLMGCQDILENVPGRSKATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTD 387

  Fly   526 ETSIEIMAVIKLDNITVGQTSWKQCSQQAWDQRFSIDLDRSRELEIGVYWRDWRSLCAVKVLRLE 590
            :.|.::.||:||||..|||||||..|.|:|||:|:::|||||||||.|||||||||||||.||||
Human   388 DLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLE 452

  Fly   591 EFIDDVRHGMALQLEPQGLLFAEVKFLNPMISQKPKLRRQRMIFNRQQAKNISRAKQMNINVATW 655
            :|:|:.||||.|.|||||.|||||.|.||:|.::|||:||:.||::||.|...||.|||||:|||
Human   453 DFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATW 517

  Fly   656 GRLLKRNAPNHVHMGSAGSGSSLTGSSPMVVGGSRDSESPISRTPSSDALVEPEPYTPGEQAQNL 720
            |||::|..|...|.|:
Human   518 GRLVRRAIPTVNHSGT------------------------------------------------- 533

  Fly   721 EFDPDAGINEHVETPGEYPDPAASGLSGMRPLSMHMQGISVLPPESPPVATGAAGRPNTLSLQMP 785
             |.|.|                                         ||.|
Human   534 -FSPQA-----------------------------------------PVPT-------------- 542

  Fly   786 GASKGQVIQGGRTAAPTTAPPPPPVLKATSTTPILDQELQDALHEFDFLSDLDSRPTTLRRLLKE 850
                                          |.|::|..:
Human   543 ------------------------------TVPVVDVRI-------------------------- 551

  Fly   851 QKMALDAGALENLLLQQQQTEQQALQQRQRQEQLRLQQFQEAQRQAILDLCVKQREPAEQTSPTL 915
                                                                             
Human   552 ----------------------------------------------------------------- 551

  Fly   916 ANQTPTFPPLASNQIMPSLNARPCQSQSPNHNEFQLQPQPPHPAQKPTNPEPPSAVEQQLHRPVL 980
                |...|.||:..:..|             :|.|:|:||        |.||.|..
Human   552 ----PQLAPPASDSTVTKL-------------DFDLEPEPP--------PAPPRASS-------- 583

  Fly   981 ILPPVVLLGGREEDRSVPPSPLVEYPEDDDEYLYRGSNENLGTRLHSSHSSSAGDRFCVEVIPQL 1045
                   ||..:|...:   .:::.|..|.|.::...|:.                  ..::|:
Human   584 -------LGEIDESSEL---RVLDIPGQDSETVFDIQNDR------------------NSILPK- 619

  Fly  1046 GKLYVGSSQQQYAQQSSPIIQEPATPTIYGNSAAAGAPQFPQPAQRQEKQPPQQQPIYANQYELN 1110
                   ||.:|         :|.||
Human   620 -------SQSEY---------KPDTP--------------------------------------- 629

  Fly  1111 VAKAAAAASVYSPSSSTTSNSNQQQQQQRRNVARGLQYRESGGLETGRAGKQPPNAGMLSMDNFR 1175
                          .|....|..|:.:.||:..| .|:                     ::.:||
Human   630 --------------QSGLEYSGIQELEDRRSQQR-FQF---------------------NLQDFR 658

  Fly  1176 LLSVLGRGHFGKVILSQLRSNNQYYAIKALKKGDIIARDEVESLLSEKRIFEVANAMRHPFLVNL 1240
            ..:||||||||||:|::.::.|:.:||||||||||:|||||:||:.||||||..|::||||||||
Human   659 CCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNL 723

  Fly  1241 YSCFQTEQHVCFVMEYAAGGDLMMHIHTDVFLEPRAVFYAACVVLGLQYLHENKIIYRDLKLDNL 1305
            ::||||::|||||||||||||||||||||||.||||||||||||||||||||:||:|||||||||
Human   724 FACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNL 788

  Fly  1306 LLDTEGYVKIADFGLCKEGMGFGDRTGTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLVG 1370
            ||||||:||||||||||||||:||||.||||||||||||||||||||||||||||||||:|||||
Human   789 LLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVG 853

  Fly  1371 ESPFPGDDEEEVFDSIVNDEVRYPRFLSLEAIAVMRRLLRKNPERRLGSSERDAEDVKKQAFFRS 1435
            ||||||||||||||||||||||||||||.|||::||||||:|||||||:||:|||||||..|||.
Human   854 ESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDVKKHPFFRL 918

  Fly  1436 IVWDDLLLRKVKPPFVPTINHLEDVSNFDEEFTSEKAQLTPPKEPRHLTEEEQLLFQDFSYTAEW 1500
            |.|..|:.:||||||:|||...|||||||:|||||...||||:|||.|:||||.:|:||.|.|:|
Human   919 IDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYIADW 983

  Fly  1501 C 1501
            |
Human   984 C 984

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pkn	NP_001097237.1	HR1_PKN_1	131..194	CDD:212012	36/62 (58%)
		HR1_PKN_2	243..309	CDD:212013	35/67 (52%)
		HR1_PKN_3	340..414	CDD:212015	38/73 (52%)
		C2	530..611	CDD:472691	57/80 (71%)
		STKc_PKN	1174..1499	CDD:270741	261/324 (81%)
PKN2	NP_006247.1	HR1_PKN_1	42..107	CDD:212012	36/64 (56%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	114..133		6/37 (16%)
		HR1	130..201	CDD:469609	37/90 (41%)
		HR1_PKN2_3	209..282	CDD:212025	39/95 (41%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	351..383		9/31 (29%)
		Necessary to rescue apical junction formation. /evidence=ECO:0000250	382..463		52/80 (65%)
		C2_PKN-like	390..473	CDD:176069	58/82 (71%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	558..584		12/61 (20%)
		STKc_PKN	657..982	CDD:270741	261/324 (81%)
		Necessary for the catalytic activity	917..977		38/59 (64%)
		Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoA	978..984		2/5 (40%)