DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pkn and Pkn2

DIOPT Version :9

Sequence 1:	NP_001097237.1	Gene:	Pkn / 35950	FlyBaseID:	FBgn0020621	Length:	1501	Species:	Drosophila melanogaster
Sequence 2:	NP_848769.2	Gene:	Pkn2 / 109333	MGIID:	109211	Length:	983	Species:	Mus musculus

Alignment Length:	1416	Identity:	564/1416 - (39%)
Similarity:	718/1416 - (50%)	Gaps:	466/1416 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   109 VLYEL---SHKYGFTENL--------PESCMSIRLEEIKEAIRREIRKELKIKEGAEKLREVAKD 162
            :|.||   |....|:||:        .::.:..:|::||:.|:||||||||||||||.||:|..|
Mouse    11 LLTELQGDSRTLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLRKVTTD 75

  Fly   163 RRSLSDVAVLVKKSKSKLAELKSELQELESQILLTSANTAVNSNGQESITACIDPNGGFLVSGAV 227
            :::|:.|..::|||..||.||..:||||.:.|::        |:.::|......|
Mouse    76 KKNLAYVDNILKKSNKKLEELHHKLQELNAHIVV--------SDPEDSTDCPRTP---------- 122

  Fly   228 GGLGGGNTALEGGAPATANDKVLASLEKQLQIEMKVKTGAENMIQ--SLGIGCDKKLLAEAHQML 290
                  :|.......:|:|::::| |:|||.||:|||.|||||||  |.|...|:||...|.|:|
Mouse   123 ------DTPNSDSRSSTSNNRLMA-LQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLL 180

  Fly   291 ADSKAKIEFLRLRIIKVKQNREQA-DRLKASRQMIDEHGQTIGGNNSSQPQSLETTLEERIEELR 354
            .|||.|||.:|::|::..|..|.| |..|                      .:.:.||.|:||||
Mouse   181 QDSKTKIEVIRMQILQAVQTNELAFDNAK----------------------PVISPLELRMEELR 223

  Fly   355 HRLRIEAAVVDGAKNVIRTLQTANRAPDKKALQEAHGRLSESSRKLDLLRYSLDLRRQELPADSP 419
            |..:||.||.:|||||::.| .:.:..|:|||.||..|.:|||:|||||:|||:.|..|||.:.|
Mouse   224 HHFKIEFAVAEGAKNVMKLL-GSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLNELPRNHP 287

  Fly   420 AAQQLKTELQIVQLSTSPAPVTYTSLQSGQAGILGGKPYQSVSSLGRCASVTGKLEVRLLGCQDL 484
            .:..:..||.:|   .||......|:.|.|         ...|:|.:.|::||.|||||:||||:
Mouse   288 KSSVVIEELSLV---ASPTLSPRQSMLSTQ---------NQYSTLSKPAALTGTLEVRLMGCQDI 340

  Fly   485 LEDVPGRSRRDKD--NNSSPGDLRSFVKGVTSRS---SSKSYSVKDETSIEIMAVIKLDNITVGQ 544
            ||:|||||:....  ...||.|.||.....||:|   ||::....|:.|.::.||:||||..|||
Mouse   341 LENVPGRSKATSVALPGWSPSDNRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQ 405

  Fly   545 TSWKQCSQQAWDQRFSIDLDRSRELEIGVYWRDWRSLCAVKVLRLEEFIDDVRHGMALQLEPQGL 609
            ||||..|.|:|||:|:::|||||||||.|||||||||||||.||||:|:|:.||||.|.|||||.
Mouse   406 TSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGT 470

  Fly   610 LFAEVKFLNPMISQKPKLRRQRMIFNRQQAKNISRAKQMNINVATWGRLLKRNAPNHVHMGSAGS 674
            |||||.|.||:|.::|||:||:.||::||.|...||.|||||:||||||::|..|...|.|
Mouse   471 LFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSG---- 531

  Fly   675 GSSLTGSSPMVVGGSRDSESPISRTPSSDALVEPEPYTPGEQAQNLEFDPDAGINEHVETPGEYP 739

Mouse   532 ----------------------------------------------------------------- 531

  Fly   740 DPAASGLSGMRPLSMHMQGISVLPPESPPVATGAAGRPNTLSLQMPGASKGQVIQGGRTAAPTTA 804
                                                                      |.:|.|.
Mouse   532 ----------------------------------------------------------TFSPQTP 538

  Fly   805 PPPPPVLKATSTTPILDQELQDALHEFDFLSDLDSRPTTLRRLLKEQKMALDAGALENLLLQQQQ 869
            .|        :|.|::|..:
Mouse   539 VP--------ATVPVVDARI--------------------------------------------- 550

  Fly   870 TEQQALQQRQRQEQLRLQQFQEAQRQAILDLCVKQREPAEQTSPTLANQTPTFPPLASNQIMPSL 934
                                                              |...|.||:..:..|
Mouse   551 --------------------------------------------------PDLAPPASDSTVTKL 565

  Fly   935 NARPCQSQSPNHNEFQLQPQPPHPAQKPTNPEPPSAVEQQLHRPVLILPPVVLLGGREEDRSVPP 999
                         :|.|:|:||        |.||.|..               ||..:|...:
Mouse   566 -------------DFDLEPEPP--------PAPPRASS---------------LGETDESSEL-- 592

  Fly  1000 SPLVEYPEDDDEYLYRGSNENLGTRLHSSHSSSAGDRFCVEVIPQLGKLYVGSSQQQYAQQSSPI 1064
             .:::.|....|.::...|:....|
Mouse   593 -RVLDIPGQGSETVFNIENDRNNLR---------------------------------------- 616

  Fly  1065 IQEPATPTIYGNSAAAGAPQFPQPAQRQEKQPPQQQPIYANQYELNVAKAAAAASVYSPSSSTT- 1128
                                                |...::|||::           |.|..:
Mouse   617 ------------------------------------PKSKSEYELSI-----------PDSGRSC 634

  Fly  1129 ---SNSNQQQQQQRRNVARGLQYRESGGLETGRAGKQPPNAGMLSMDNFRLLSVLGRGHFGKVIL 1190
               ...:.::.|||      .|:                     |:.:||..:||||||||||:|
Mouse   635 WGVGELDDKRAQQR------FQF---------------------SLQDFRCCAVLGRGHFGKVLL 672

  Fly  1191 SQLRSNNQYYAIKALKKGDIIARDEVESLLSEKRIFEVANAMRHPFLVNLYSCFQTEQHVCFVME 1255
            ::.:..|:.:||||||||||:|||||:||:.||||||..|::||||||||::||||::|||||||
Mouse   673 AEYKHTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVME 737

  Fly  1256 YAAGGDLMMHIHTDVFLEPRAVFYAACVVLGLQYLHENKIIYRDLKLDNLLLDTEGYVKIADFGL 1320
            ||||||||||||||||.||||||||||||||||||||:||:|||||||||||||||:||||||||
Mouse   738 YAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGL 802

  Fly  1321 CKEGMGFGDRTGTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLVGESPFPGDDEEEVFDS 1385
            ||||||:||||.||||||||||||||||||||||||||||||||:||||||||||||||||||||
Mouse   803 CKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDS 867

  Fly  1386 IVNDEVRYPRFLSLEAIAVMRRLLRKNPERRLGSSERDAEDVKKQAFFRSIVWDDLLLRKVKPPF 1450
            |||||||||||||.|||::||||||:|||||||:.|:|||||||..|||...|..|:.:||||||
Mouse   868 IVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVKKHPFFRLTDWSALMDKKVKPPF 932

  Fly  1451 VPTINHLEDVSNFDEEFTSEKAQLTPPKEPRHLTEEEQLLFQDFSYTAEWC 1501
            ||||...|||||||:|||||...||||:|||.|.||||.:|.||.|.|:||
Mouse   933 VPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEMFHDFDYVADWC 983

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkn	NP_001097237.1	HR1_PKN_1	131..194	CDD:212012	35/62 (56%)
		HR1_PKN_2	243..309	CDD:212013	35/67 (52%)
		HR1_PKN_3	340..414	CDD:212015	37/73 (51%)
		C2	530..611	CDD:301316	57/80 (71%)
		STKc_PKN	1174..1499	CDD:270741	260/324 (80%)
		S_TKc	1174..1433	CDD:214567	217/258 (84%)
Pkn2	NP_848769.2	HR1_PKN_1	42..107	CDD:212012	35/64 (55%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	107..135		6/51 (12%)
		HR1_PKN2_2	128..201	CDD:212021	36/73 (49%)
		HR1_PKN2_3	209..282	CDD:212025	38/95 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	351..382		10/30 (33%)
		Necessary to rescue apical junction formation	381..462		52/80 (65%)
		C2_PKN-like	389..472	CDD:176069	58/82 (71%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	553..588		15/70 (21%)
		STKc_PKN	656..981	CDD:270741	260/324 (80%)
		S_TKc	661..915	CDD:214567	215/253 (85%)
		Necessary for the catalytic activity. /evidence=ECO:0000250	916..976		38/59 (64%)
		Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoA. /evidence=ECO:0000250	977..983		2/5 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167839291
Domainoid	1	1.000	460	1.000	Domainoid score	I486
eggNOG	1	0.900	-	-		E1_KOG0694
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2054
Inparanoid	1	1.050	899	1.000	Inparanoid score	I382
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG49182
OrthoDB	1	1.010	-	-		D241576at33208
OrthoFinder	1	1.000	-	-		FOG0001458
OrthoInspector	1	1.000	-	-		otm43267
orthoMCL	1	0.900	-	-		OOG6_103771
Panther	1	1.100	-	-	O	PTHR24356
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	1	1.000	-	-		X914
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.800