DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sec31 and SEC31

DIOPT Version :9

Sequence 1:	NP_001286215.1	Gene:	Sec31 / 35877	FlyBaseID:	FBgn0033339	Length:	1260	Species:	Drosophila melanogaster
Sequence 2:	NP_010086.2	Gene:	SEC31 / 851332	SGDID:	S000002354	Length:	1273	Species:	Saccharomyces cerevisiae

Alignment Length:	1403	Identity:	357/1403 - (25%)
Similarity:	577/1403 - (41%)	Gaps:	293/1403 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MKIKELQKTVNIAWSPAQQQQILLAAGTAAQQFDSN--ANSTLELYSPNFSDATYDLELRASVSS 63
            :|:.|..:|...|||  ..:..||.:||.:...|:|  .:|:|||:|...:|:...:   ||:..
Yeast     2 VKLAEFSRTATFAWS--HDKIPLLVSGTVSGTVDANFSTDSSLELWSLLAADSEKPI---ASLQV 61

  Fly    64 QYKFQKLIWSPVGPHPSGLIVGGCEAGQINIYSADKLLAGKEEPLLKRQDKHTGAVSGLDFNPFQ 128
            ..||..|.||    |.:.:|.|..:.|.:.:||.::  |......:.|...|:.:|..:.||..|
Yeast    62 DSKFNDLDWS----HNNKIIAGALDNGSLELYSTNE--ANNAINSMARFSNHSSSVKTVKFNAKQ 120

  Fly   129 NNLLASCASESEILIWDLN----NPE--TSMSPGTKTQPLEDVKNVAWNRQVQHILASVFSTR-C 186
            :|:|||..:..||.|||:|    :|.  |.::||.....:::|.::|||:.:.|:.||..|:. .
Yeast   121 DNVLASGGNNGEIFIWDMNKCTESPSNYTPLTPGQSMSSVDEVISLAWNQSLAHVFASAGSSNFA 185

  Fly   187 VIWDLRKSQQIIKLS----DSQSRVRWHAIEWHPEAATQVWLASEDDQAPVVQLWDLRYATAPAK 247
            .||||:..:::|.||    :|..:.:...:||||:.:|:|..|:..|..|.:.:||||.|..|.:
Yeast   186 SIWDLKAKKEVIHLSYTSPNSGIKQQLSVVEWHPKNSTRVATATGSDNDPSILIWDLRNANTPLQ 250

  Fly   248 TY-QIHERGVLGMSWCLQDNDLMVSCGKDNRIYCWNPNTKIPEGEILSEVATTASWYSDVQFCPR 311
            |. |.|::|:|.:.||.||..|::|.|:||.:..|||.:    .|.||:.....:|....:|.|.
Yeast   251 TLNQGHQKGILSLDWCHQDEHLLLSSGRDNTVLLWNPES----AEQLSQFPARGNWCFKTKFAPE 311

  Fly   312 NPALIASASLEGAVSIYSLHGGTHHL--VQTANKIADSFPGM-DQFAQEPIPQQATQVVYHDLAH 373
            .|.|.|.||.:..:.:.:|...|:.|  .:|..|..:|.... :..::|...::.|  |:|  ..
Yeast   312 APDLFACASFDNKIEVQTLQNLTNTLDEQETETKQQESETDFWNNVSREESKEKPT--VFH--LQ 372

  Fly   374 APKWMKRPCGVA-FGFGGKLVSFNGTSKTVKVQQLTTESALVDRANALERSLVDANYSDYCRQRA 437
            ||.|...|...| :.||||||......|.|.:  ...:.:.::....|..:|...::.....||
Yeast   373 APTWYGEPSPAAHWAFGGKLVQITPDGKGVSI--TNPKISGLESNTTLSEALKTKDFKPLINQR- 434

  Fly   438 DETPDQHGRYIWYFIKANFELNPKEEMLNLLGYNKDDIDSKFNKFIKETDGNSHSDVDTLTTRIS 502
                         .:|...::|  ||..|||  .|..:|.. .:|:||                :
Yeast   435 -------------LVKVIDDVN--EEDWNLL--EKLSMDGT-EEFLKE----------------A 465

  Fly   503 TLTHSDSSEVECDQSTNYSTDNSQTQDNKFDGNAIDSQNHNDFATPPRPQFKVPSGDHS-----D 562
            ....:|.|:.:.|.       |::.:|   ||.....|...:|.         |.||.|     :
Yeast   466 LAFDNDESDAQDDA-------NNEKED---DGEEFFQQIETNFQ---------PEGDFSLSGNIE 511

  Fly   563 SLIAEAILTGNVDAAVELCLEAQRIPEALIIASTAGIETLNRT-QTRYLLQ--QKNELSHVISAL 624
            ..|::.:::||:.:||:..||...:.||::||..:..|.|..: :..|..:  .|:.||.::.::
Yeast   512 QTISKNLVSGNIKSAVKNSLENDLLMEAMVIALDSNNERLKESVKNAYFAKYGSKSSLSRILYSI 576

  Fly   625 VSRDWLDFVNRCTVDSWKEALVAALKHSERKVVDICER------LGDRLLSECASSAEFTRNAML 683
            ..|:..|.|....|..|| .:..|:::....  ||.:|      |||||     ......::::.
Yeast   577 SKREVDDLVENLDVSQWK-FISKAIQNLYPN--DIAQRNEMLIKLGDRL-----KENGHRQDSLT 633

  Fly   684 CYICAGSIDKLVTAWYQ-----LKRLEQQNPG-YKPNTTELQDLAEMVMLMCKSLEQQGISVDLA 742
            .|:.|||:||:.:.|..     ..:|::.|.. |:.::..|.:..|...:....:.... :::..
Yeast   634 LYLAAGSLDKVASIWLSEFPDLEDKLKKDNKTIYEAHSECLTEFIERFTVFSNFINGSS-TINNE 697

  Fly   743 GRFAGFLTEYGGLLASQGALTAALQYITTLGGGAAASESDVLQSLRDR--INNAVNLDYTQPQAA 805
            ...|.|| |:..|..|.|....|.:::.:|     .|:::.:::.:.|  |.:..:|....|..|
Yeast   698 QLIAKFL-EFINLTTSTGNFELATEFLNSL-----PSDNEEVKTEKARVLIASGKSLPAQNPATA 756

  Fly   806 VAAAPHGYQPTRGRGSFSQPQPSAPMPYGQPPNQYAQPSWPAQVPPLQTSYPGAQQPQAPPQIFN 870
                      |..:..::..:.:..:|....|...:..|.|:...|.....|||.....||:.:.
Yeast   757 ----------TTSKAKYTNAKTNKNVPVLPTPGMPSTTSIPSMQAPFYGMTPGASANALPPKPYV 811

  Fly   871 P-----QPVKPEPLAQPPRPLSNAS------------SSGGPPPAGGASSS-GGSGLSGRSKYVL 917
            |     .||..|....||...|.||            :|..|||...|::: ..:.:|..|   :
Yeast   812 PATTTSAPVHTEGKYAPPSQPSMASPFVNKTNSSTRLNSFAPPPNPYATATVPATNVSTTS---I 873

  Fly   918 DPSVAAPTSSYGMP----YNPVPAPVPSAVPFGGGGVPGPAPQ------------PASVPTYNSN 966
            ..:..||... |||    ||...:.:||..|.  ..|.|..|.            |..|....|.
Yeast   874 PQNTFAPIQP-GMPIMGDYNAQSSSIPSQPPI--NAVSGQTPHLNRKANDGWNDLPLKVKEKPSR 935

  Fly   967 AFNTNPIAAAQP---YNPSPL--MPAQQNQFVPGVNPIETMPPPAVPPVIQNVQRNPTPPPGWND 1026
            |   ..::.|.|   ..|:||  :||         |...|||||.       :.|.|:.....:.
Yeast   936 A---KAVSVAPPNILSTPTPLNGIPA---------NAASTMPPPP-------LSRAPSSVSMVSP 981

  Fly  1027 PPALKSSRAPKPKPVVE-PSAAIFHPLFPVDPNQ----------NQ----------NGYVDPSQY 1070
            ||..|:||.|......| |.|:|.:|..|...:|          ||          |.|..|.|.
Yeast   982 PPLHKNSRVPSLVATSESPRASISNPYAPPQSSQQFPIGTISTANQTSNTAQVASSNPYAPPPQQ 1046

  Fly  1071 QHAAPL-GGVP--------NTY------------YNPAA-FNNNPQQQQQHQQSYLQPQQPAP-- 1111
            :.|.|| ||||        |.|            |.|.. :.||.............|..|.|
Yeast  1047 RVATPLSGGVPPAPLPKASNPYAPTATTQPNGSSYPPTGPYTNNHTMTSPPPVFNKPPTGPPPIS 1111

  Fly  1112 ---------GIQQPNWQGQQQTIGYTEQPAPIQQQRQPAPPKEKPPL---------------PEE 1152
                     .|:|...||       ...|..:.....|..|.:.|.|               |.:
Yeast  1112 MKKRSNKLASIEQNPSQG-------ATYPPTLSSSASPLQPSQPPTLASQVNTSAENVSHEIPAD 1169

  Fly  1153 YIYLQTVLEELKNRCLGATNDPRTKRKF----VDVVKRLENL-YDCLRDGKLTPTTVQYLNQIVQ 1212
            .   |.:::.||......|  |.|.:::    .|..|||:.| |...:...||..|:..|:.:|.
Yeast  1170 Q---QPIVDFLKEELARVT--PLTPKEYSKQLKDCDKRLKILFYHLEKQDLLTQPTIDCLHDLVA 1229

  Fly  1213 YIQISDYANAMEIHTQIAFGTDFAQCAG-FMQGLKVLL 1249
            .::...|..||.||..||  |:.||..| ::.|:|.|:
Yeast  1230 LMKEKKYKEAMVIHANIA--TNHAQEGGNWLTGVKRLI 1265

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sec31	NP_001286215.1	WD40 repeat	11..61	CDD:293791	16/51 (31%)
		WD40	<35..334	CDD:225201	101/312 (32%)
		WD40 repeat	68..114	CDD:293791	11/45 (24%)
		WD40	<80..329	CDD:295369	85/260 (33%)
		WD40 repeat	119..156	CDD:293791	16/42 (38%)
		WD40 repeat	166..202	CDD:293791	14/40 (35%)
		WD40 repeat	209..250	CDD:293791	16/41 (39%)
		WD40 repeat	256..296	CDD:293791	16/39 (41%)
		WD40 repeat	303..328	CDD:293791	7/24 (29%)
		ACE1-Sec16-like	<570..>690	CDD:301350	33/128 (26%)
		RCR	<811..>873	CDD:304939	12/66 (18%)
SEC31	NP_010086.2	WD40 repeat	12..61	CDD:293791	17/53 (32%)
		WD40	17..327	CDD:421866	105/322 (33%)
		WD40 repeat	66..106	CDD:293791	11/45 (24%)
		WD40 repeat	111..154	CDD:293791	16/42 (38%)
		WD40 repeat	164..200	CDD:293791	12/35 (34%)
		WD40 repeat	212..253	CDD:293791	16/40 (40%)
		WD40 repeat	260..297	CDD:293791	16/40 (40%)
		WD40 repeat	303..327	CDD:293791	7/23 (30%)
		PHA03247	<772..1172	CDD:223021	103/434 (24%)
		Sec31	899..946	CDD:402928	12/51 (24%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346012
Domainoid	1	1.000	51	1.000	Domainoid score	I2881
eggNOG	1	0.900	-	-		E1_KOG0307
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	278	1.000	Inparanoid score	I572
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54150
OrthoFinder	1	1.000	-	-		FOG0002042
OrthoInspector	1	1.000	-	-		oto99557
orthoMCL	1	0.900	-	-		OOG6_101250
Panther	1	1.100	-	-	LDO	PTHR13923
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2285
SonicParanoid	1	1.000	-	-		X1512
TreeFam	1	0.960	-	-
	14	13.790