Protein Alignment Cirl and adgrl2a

DIOPT Version :9

• Sequence 1: NP_001260807.1
• Gene: Cirl / 35846
• FlyBaseID: FBgn0033313
• Length: 1711
• Species: Drosophila melanogaster
• Sequence 2: XP_021335387.1
• Gene: adgrl2a / 57987
• ZFINID: ZDB-GENE-000607-49
• Length: 1520
• Species: Danio rerio
• Alignment Length: 1608
• Identity: 369/1608 - (22%)
• Similarity: 581/1608 - (36%)
• Gaps: 454/1608 - (28%)

  Fly    25 ACEGKKLTIECDPGDVINLIRANYGRFSITICN-DHGNVEWSVNCMFPKSLSVLNSRCAHKQSCG 88
            :|||..:.:.|...|||.:..|||||....||: |...:| ::||..|.:..:::.||.::..|.
Zfish    53 SCEGYPIDLRCPGSDVIMIETANYGRTDDKICDADPFQME-NINCYLPDAFKIMSQRCNNRTQCI 116

  Fly    89 VLAATSMFGDPCPGTHKYLEAHYQCIS---------AAQTSTTTNRPS--------------PP- 129
            |:....:|.||||||:||||..|:|:.         ...|......||              .|
Zfish   117 VITGPDVFPDPCPGTYKYLEVQYECVPYKVEQKVFVCPGTLKAVGDPSFLYEAEQQAGSWCKDPL 181

  Fly   130 ---------PW--------VLSNGPPIFGN--------------GSG-LIHPPGVGAGAPPPPRL 162
                     ||        :..|....|.|              |:| :::...|.........:
Zfish   182 QAGDKVYFMPWTPYRTDTLIEYNSLDDFQNSRQTTTYKLPHRVDGTGFVVYDGAVFFNKERTRNI 246

  Fly   163 PTLPGVVGISGNPGLFNVPPQHTA---------------------VTHSTPSSS----TTAVGGG 202
            ........|.....:.|....|..                     |.::|..::    .:.:...
Zfish   247 VKFDLRTRIKSGEAIINNANYHDTSPYKWGGKTDIDLAVDENGLWVIYATEQNNGMMVISQLNPY 311

  Fly   203 RLKGGATSTTT----------------------------------------TKHPAGRHDGLPPP 227
            .|:..||..||                                        ||...|.:..:..|
Zfish   312 TLRFEATWETTYDKRSASNAFMICGVLYVVRSTYEDNESEVSKSLIDYMYNTKLNHGEYVDIHFP 376

  Fly   228 PQLHH----HHNHHGED--------------TASPTKPSSKLPAGGNATSP------SNTRILTG 268
            .|..:    .:|.....              ...|..|:...|....:|||      :.|...|.
Zfish   377 NQYQYIAAVEYNPRDNQLYVWNNFYILRYNLVFGPPDPAHAPPISEASTSPKVLKPTTTTTTTTQ 441

  Fly   269 VGGSGTDDGTLLTT-----KSSPNRPPGTAASGSVVPGNGSVVRTINNINLNAAGMSGGDDESKL 328
            ..|..|   |:.||     ..:|..||       |:|      :|....:|.:.      ...:.
Zfish   442 PKGQVT---TITTTGTRAVNKTPKSPP-------VLP------QTTTPPSLESF------PPPER 484

  Fly   329 FCGPTHARNLYWNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGG 393
            ||.....|::.|..|:.|.:..:|||.|..|.|.:.|||                       |.|
Zfish   485 FCESVELRDILWPQTQRGMLVERPCPKGTRGTASYLCVL-----------------------STG 526

  Fly   394 DCLHNSSSCEPPVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLEMRVNQRDSSLISIAND 458
                                        ||:|..|||:.|.|.|:|.:..::...::: .::||:
Zfish   527 ----------------------------TWNPKGPDLSNCTSHWVNQVAQKIRSGENA-ANLANE 562

  Fly   459 MSEVTSSKTLYGGDMLVTTKIIQTV-------------SEKMMHDKETFPDQRQREAMIMELLHC 510
            :::.|.. .::.||:..|.::::.:             |||....: :|...::||......:..
Zfish   563 LAKHTKG-AIFAGDISSTMRLMEQLVDILDAQLQELRPSEKDSTGR-SFNKLQKRERTCRAYMKA 625

  Fly   511 VVKTGSNLLDESQLSSWLDLNPEDQMRVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVR 575
            :|.|..|||....|.||.|:|..:|...||.||..||..||:|||.::....|.....||:|.|.
Zfish   626 IVDTVDNLLRPEALKSWRDMNTTEQTHAATMLLDTLEEGAFVLADNLMEPAIVKVPASNIILDVY 690

  Fly   576 VLETKTIQSSVVFPDSDQW----PLSSDRIELPRAALIDNSEGGLVRIVFAAFDRLESILKPSYD 636
            ||.|........||.|.:.    .|||:.::|       ||..|:.::|||.:..|...|.
Zfish   691 VLSTDGQVQDFRFPQSGRGGISIQLSSNTVKL-------NSRNGVAKLVFALYKNLGQFLS---- 744

  Fly   637 HFDLKSSRSYVRNTAILSNDSDVNAGEIQQRLRILNSKVISASLGK-GRHIQLSQPITLTLKHLK 700
                       ...|.:....:||...:..   .:||.||:||:.| ...:.:|:|:..||:|:.
Zfish   745 -----------TENATMKLGQEVNGRNLSV---AVNSDVIAASINKESSRVFISEPVIFTLEHID 795

  Fly   701 TENVTNPTCVFWNYIDHA----WSANGCSLESTNRTHSVCSCNHLTNFAILMD-----VVDEHQH 756
            .::..|..|.||||.:..    ||..||.|...|::|:.|||:||||||:||.     ..|:.|.
Zfish   796 MQHYYNSNCSFWNYSERTMMGYWSTQGCKLIHANKSHTTCSCSHLTNFAVLMAHRELLGADKVQE 860

  Fly   757 SLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLFNGVFVKSARTSIYTSIYLCLLAIELLFLLG 821
            .|.::..   |:.|.:|: ||   :.:::.|...|.|  ::|.|.:|:.::.:.|...||:||:|
Zfish   861 LLLSVIS---RVGIVVSL-IC---LAMSIFTFCFFRG--LQSDRNTIHKNLCINLFIAELIFLIG 916

  Fly   822 IEQTETSIFCGFITIFLHCAILSGTAWFCYEAFHSYSTLTSDELLLEV--DQTPKVNCYYLLSYG 884
            |:.||..:.|..|...||...|:..||.|.|....|.      :|:||  .:..:...:|:..|.
Zfish   917 IDMTEPKLACSIIAGILHYFFLASFAWMCLEGVQLYL------MLVEVFESEYSRRKYFYMAGYL 975

  Fly   885 LSLSVVAISLVIDPSTYTQNDYCVLMEANALFYA-----TFVIPVLVFFVAAIGYTFLSWIIMCR 944
            |...||.:|..:|..:|.....|.|...|...::     ||:|.:.:.|:....|..:
Zfish   976 LPAVVVGVSAAVDFRSYGTKKACWLRMDNHFIWSFIGPVTFIIMLNLIFLLITMYKMM------- 1033

  Fly   945 KSRTGLKTKEHTRLASVRFDIRC------------------------SFVFLLLLSAVWCSAYFY 985
            |..|.|| .:.:||..:.....|                        .|..|.||...|....|:
Zfish  1034 KHSTSLK-PDSSRLEHINNYRVCDGYYNSDLPGYEDNKPFIKSWVMGGFALLCLLGLTWSFGLFF 1097

  Fly   986 LRGAKMDDDTADVYGYCFICFNTLLGLYIFVFHCIQNEKIRREYRKYVRQHAWLPKCLRCSKTSI 1050
            :      .:::.|..|.|..|||..|::||.|||:..:|:||||.|.:| |.:.  |.|.:..|.
Zfish  1098 I------SESSVVLAYLFTIFNTFQGMFIFTFHCLLQKKVRREYSKCLR-HTYC--CSRITTESS 1153

  Fly  1051 SSGIVTGNGPTAGTLCSVSTSKKPKLPLGVSEEAHDDPQQQQQTPVPITEDAIMGATSDCELNEA 1115
            .|...|....|:....|.:.|:..::        .:|..::|.....|:.|  :.:||  .||:.
Zfish  1154 HSSTKTSTTRTSARYSSGTQSRIRRM--------WNDTVRKQSESSFISGD--INSTS--TLNQG 1206

  Fly  1116 QQRRTLKSGLMTGTLQAPPQTLGGHVVLERGSTLRSTGHASP------------TSSAGSTHLIF 1168
            :.:..        |:..|.:.:.|:.:| ....||..|..:|            |.:....|...
Zfish  1207 KTKHK--------TISEPLEGMTGNYLL-TNPLLRPQGTNNPYNTLLAETIVCNTPTVPVFHSPV 1262

  Fly  1169 AHKQQQQQQQQG---------PLGESYYHQPDYYSWKQPSTGTG----GLK---TPRE------- 1210
            .:::.:.....|         ||..::.:.   ||....|.|..    ||.   |..|
Zfish  1263 TYRETRHSVTSGRDTNTFGTLPLNGNFNNS---YSLHNYSDGMQVVDCGLSLNDTAFEKMIISEL 1324

  Fly  1211 YYNNAGAAASSPQQAHEVFYWTQKPNSGHNGKKK-----RGAGGVPASPSGSL------------ 1258
            .:||..||.|                  ..|.||     ||...|.|..|..:
Zfish  1325 VHNNLRAAES------------------RIGTKKGAGISRGEEPVVAETSSLVQVGGDSEMERLG 1371

  Fly  1259 ----HSRTAAASQV------LFYPSYKKTKPGQPTGYPQ-YAEALDPPLATG----NAAAYYQQQ 1308
                |.||..|..:      |.|.:.::.:|.....:|| |...|.||..|.    |..:.|...
Zfish  1372 LHHTHPRTLEAPLIPQRTHSLLYHTQERARPEAEASHPQSYIGQLPPPAETALPSPNRDSVYTSM 1436

  Fly  1309 QQLRRQQLHQQQQQQQQQQLSSDEEQAEQHAHLLHLQRRAGSQQQLPA 1356
            ..||.... .:......|.||..:....:..:...:. ..||.|||.|
Zfish  1437 PNLRDSPF-SESSLDIVQDLSPSKNSPNEDVYYKSMP-NLGSGQQLHA 1482

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cirl	NP_001260807.1	Gal_Lectin	33..113	CDD:280328	32/80 (40%)
		GAIN	453..680	CDD:293098	65/243 (27%)
		GPS	705..752	CDD:197639	24/55 (44%)
		7tm_4	763..1014	CDD:304433	69/281 (25%)
adgrl2a	XP_021335387.1	Gal_Lectin	61..141	CDD:307994	32/80 (40%)
		OLF	155..411	CDD:321983	28/255 (11%)
		HormR	482..545	CDD:214468	26/113 (23%)
		GAIN	555..776	CDD:318649	66/248 (27%)
		GPS	800..851	CDD:197639	24/50 (48%)
		7tm_GPCRs	860..1141	CDD:333717	82/310 (26%)
		TM helix 1	863..887	CDD:320095	6/30 (20%)
		TM helix 2	896..917	CDD:320095	6/20 (30%)
		TM helix 3	927..949	CDD:320095	8/21 (38%)
		TM helix 4	968..984	CDD:320095	5/15 (33%)
		TM helix 5	1003..1026	CDD:320095	5/22 (23%)
		TM helix 6	1074..1099	CDD:320095	6/30 (20%)
		TM helix 7	1103..1128	CDD:320095	12/24 (50%)
		Latrophilin	1140..1520	CDD:308136	79/390 (20%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	76	1.000	Domainoid score	I8871
eggNOG	1	0.900	-	-		E1_KOG4193
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	369	1.000	Inparanoid score	I2092
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000766
OrthoInspector	1	1.000	-	-		otm24884
orthoMCL	1	0.900	-	-		OOG6_102405
Panther	1	1.100	-	-	O	PTHR12011
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X858
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	11	10.820