Protein Alignment Cirl and ADGRA2

DIOPT Version :10

• Sequence 1: NP_001260807.1
• Gene: Cirl / 35846
• FlyBaseID: FBgn0033313
• Length: 1711
• Species: Drosophila melanogaster
• Sequence 2: XP_011542783.1
• Gene: ADGRA2 / 25960
• HGNCID: 17849
• Length: 1361
• Species: Homo sapiens
• Alignment Length: 1622
• Identity: 314/1622 - (19%)
• Similarity: 509/1622 - (31%)
• Gaps: 585/1622 - (36%)

  Fly    64 WSVNCMFPKS-------LSVLNSRCAHKQSCGVLAATSMFGDPCPGTHKYLEAHYQCISAAQTST 121
            |.:..:.|::       ||:.:.:|:.::..|:..     |.|.|...:.:     |...
Human    22 WLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSG-----GVPGPARRRVV-----CSGG----- 71

  Fly   122 TTNRPSPP-PWVLSNGP--------PIFG--NGSGL----------------IHPPGVGAGAPPP 159
              :.|.|| |.:|.||.        .|.|  |||.|                ...||...|....
Human    72 --DLPEPPEPGLLPNGTVTLLLSNNKITGLRNGSFLGLSLLEKLDLRNNIISTVQPGAFLGLGEL 134

  Fly   160 PRL------------PTLPGV-----VGISGN------PGLFNVPPQHTAVTHST---------- 191
            .||            .|..|:     :.||||      ||:|:..|....|...|
Human   135 KRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLR 199

  Fly   192 ---PSSSTTAVGGGRLKGGATSTTTTKHPAGRH--------------DGLPPPPQLHHHHNHHGE 239
               |.:...::       ..:..|...:|:..|              :|   ..:||.||
Human   200 WLLPWAQNRSL-------QLSEHTLCAYPSALHAQALGSLQEAQLCCEG---ALELHTHH----- 249

  Fly   240 DTASPTKPS--------SKLPAGGNATSPSN-TRI--------LTGVGGSG-------TDDGTLL 280
                 ..||        .:||...:|:...| |||        :.|...:|       ..|.|.:
Human   250 -----LIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFI 309

  Fly   281 TTKSSPNRPPGTAASG------SVVPGNGSVVRTINNINLNAAGMSGGDDESKLFCGPTHARNLY 339
            |::.:.:. .|..|||      |:..||.|  :.:..:.|         :.|..:|......|
Human   310 TSELTLSH-IGVWASGEWECTVSMAQGNAS--KKVEIVVL---------ETSASYCPAERVAN-- 360

  Fly   340 WNMTRVGDVNVQPCPGGAAGIAKWRCVLMKRIPDSGYDEYDDDISSTTPAPSGGDCLHNSSSCEP 404
                ..||..   .|...|||..::..|          :|     ..|..|.||..
Human   361 ----NRGDFR---WPRTLAGITAYQSCL----------QY-----PFTSVPLGGGA--------- 394

  Fly   405 PVSMAHKVNQRLRNFEPTWHPATPDLTQCRSLWLNNLE--------MRVNQRDSSLISIANDMSE 461
            |.:.|.:...|...:||      .|.:.|  |:.|::.        |.:|.  |:.:::|:.:..
Human   395 PGTRASRRCDRAGRWEP------GDYSHC--LYTNDITRVLYTFVLMPINA--SNALTLAHQLRV 449

  Fly   462 VTSSKTLYGGDMLVTTKIIQTVSEKMMHDKETFPDQRQREAMIMELLHCVVKTGSNLL--DESQL 524
            .|:....: .||:....:.|.:.:.:     .:.||      |.||:..:|...|||:  ||..|
Human   450 YTAEAASF-SDMMDVVYVAQMIQKFL-----GYVDQ------IKELVEVMVDMASNLMLVDEHLL 502

  Fly   525 SSWLDLNPEDQM---------RVATSLLTGLEYNAFLLADTIIRERSVVQKVKNILLSVRVLETK 580
              || ...||:.         |:..:.|:....:..:.|..:..|..:::....:.|:....:.:
Human   503 --WL-AQREDKACSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRR 564

  Fly   581 TIQSSV---------VFPDSDQWPLSSDRIEL------PRAAL----IDNSEG-GLVRIVFAAFD 625
              :..|         ..|..:..|.:..::..      |..:|    |.||.. ..:::..:.|.
Human   565 --EGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFS 627

  Fly   626 RLESILKPSYDHFDLKSSRSYVRNTAIL---SNDSDVNAGEIQQRLRILNSKVISASLGKGRHIQ 687
            .|.:.|.|.... |........||..:.   ||.|...|....:|..:....:.:.:.|.|.. .
Human   628 SLPAALAPPVPP-DCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVG-N 690

  Fly   688 LSQPITLTLKHLKTENVTNPTCVFWNYID----HAWSANGCSLESTNRTHSVCSCNHLTNFAILM 748
            |::|:.::|:|  ......|...:|:...    ..|::.||.|.|:....|...|.||.|.|:||
Human   691 LTEPVAVSLRH--WAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLM 753

  Fly   749 DVVDEHQHSLFTMFDGNMRIFIYISIGICVVFIVIALLTLKLF--------NGVFVKSARTSIYT 805
            ::      |.|....|.....::     .||:...|||.|.||        |...::.:|...:.
Human   754 EL------SAFPREVGGAGAGLH-----PVVYPCTALLLLCLFATIITYILNHSSIRVSRKGWHM 807

  Fly   806 SIYLC--LLAIELLFLLGIEQTETSIFCGFITIFLHCAILSGTAWFCYEA--FHSYSTL------ 860
            .:.||  :.....:|..||..|...:.|..:.|.||.:.||...|...:|  .|...|.
Human   808 LLNLCFHIAMTSAVFAGGITLTNYQMVCQAVGITLHYSSLSTLLWMGVKARVLHKELTWRAPPPQ 872

  Fly   861 -------TSDELLLEVDQTPKVNC-------------YYLLSYGLSLSVVAISLVIDPSTYTQND 905
                   |...:|..:...|.:..             :||::.|:.|.:..|:..::...|..:.
Human   873 EGDPALPTPSPMLRYILSIPALQLGRDSNAGVGNLPRFYLIAGGIPLIICGITAAVNIHNYRDHS 937

  Fly   906 -YCVLMEANALFYATFVIPVLVFFVAAIGYTFLSWI-IMCR---------------KSRTGLKTK 953
             ||.|:...:|  ..|.|||.:..:       ::|| .:|.               .||..|:..
Human   938 PYCWLVWRPSL--GAFYIPVALILL-------ITWIYFLCAGLRLRGPLAQNPKAGNSRASLEAG 993

  Fly   954 EH----TRL----------------ASVRFD---------------------IRCSFVFL----- 972
            |.    |||                .|.|..                     :...|::|
Human   994 EELRGSTRLRGSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLYLAMWAC 1058

  Fly   973 --LLLSAVW-----CSAYFYLRGAKMDDDTADVYGYCFICFNTLLGLYIFVFHCIQNEKIRREYR 1030
              |.:|..|     ||.               :||..    .:.|||::|..||.:...:|..:|
Human  1059 GALAVSQRWLPRVVCSC---------------LYGVA----ASALGLFVFTHHCARRRDVRASWR 1104

  Fly  1031 ------KYVRQHAWLPKCLRCSKTSISSGIVTGNGPTAGTLCSVSTSKKPKLPLGVSEEAHDDPQ 1089
                  .....|| .|:.|..:....|.  |.|.||.     |:.:|     |.|.|  .|
Human  1105 ACCPPASPAAPHA-PPRALPAAAEDGSP--VFGEGPP-----SLKSS-----PSGSS--GH---- 1150

  Fly  1090 QQQQTPVPITEDAIMGATSDCELNEAQ--QRRTLKSGLMTGTLQAPPQTLGGHVVLERGSTLRST 1152
                   |:       |...|:|...|  |.:..::|...|. :..|:..|         |..:.
Human  1151 -------PL-------ALGPCKLTNLQLAQSQVCEAGAAAGG-EGEPEPAG---------TRGNL 1191

  Fly  1153 GHASPTSSAGSTHLIFAHKQQQQQQQQGPLGESYYHQPDYYSWKQPSTGTGGLKTPREYYNNAGA 1217
            .|..|.:   ..|...|||.:.:..:.|                 .:.|...||..|      |.
Human  1192 AHRHPNN---VHHGRRAHKSRAKGHRAG-----------------EACGKNRLKALR------GG 1230

  Fly  1218 AASSPQQAHEVFYWTQKPNSGHNGKKKRGAGGVPASPSGSLHSRTAAASQVLFYPSYKKTKPG-- 1280
            ||                          ||..:.:|.|||||:     |....|....:..||
Human  1231 AA--------------------------GALELLSSESGSLHN-----SPTDSYLGSSRNSPGAG 1264

  Fly  1281 -QPTGYPQYAEALDPPLATGNAAAYYQQQQQLRRQQLHQQQQQQQQQQLSSDEEQAEQHAHLLHL 1344
             |..|.|.    |.|...:..:||...:..:..
Human  1265 LQLEGEPM----LTPSEGSDTSAAPLSEAGRAG-------------------------------- 1293

  Fly  1345 QRRAGSQQQLPAPPPHMAQYQQEFMQRQYRNKHSNCDLGMGDAYYNQGSVGGADGGPVYEEI 1406
            |||:.|:..|...    ...::|..:|.|.              .|..|:.||..|..|:::
Human  1294 QRRSASRDSLKGG----GALEKESHRRSYP--------------LNAASLNGAPKGGKYDDV 1337

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Cirl	NP_001260807.1	Gal_Rha_Lectin_dCirl	22..113	CDD:438687	9/55 (16%)
		GAIN	451..682	CDD:465137	46/264 (17%)
		GPS	705..752	CDD:197639	15/50 (30%)
		7tmB2_CELSR_Adhesion_IV	763..1035	CDD:320557	73/385 (19%)
		TM helix 1	765..790	CDD:320557	6/24 (25%)
		TM helix 2	801..823	CDD:320557	4/23 (17%)
		TM helix 3	832..859	CDD:320557	8/28 (29%)
		TM helix 4	875..895	CDD:320557	5/32 (16%)
		TM helix 5	913..942	CDD:320557	7/29 (24%)
		TM helix 6	960..987	CDD:320557	9/59 (15%)
		TM helix 7	997..1022	CDD:320557	8/24 (33%)
		Herpes_TAF50	<1219..1365	CDD:308764	25/148 (17%)
ADGRA2	XP_011542783.1	leucine-rich repeat	86..109	CDD:275378	6/22 (27%)
		LRR	<88..>232	CDD:443914	27/150 (18%)
		LRR_8	88..144	CDD:404697	11/55 (20%)
		leucine-rich repeat	110..133	CDD:275378	3/22 (14%)
		leucine-rich repeat	134..157	CDD:275378	4/22 (18%)
		leucine-rich repeat	158..181	CDD:275378	7/22 (32%)
		leucine-rich repeat	182..194	CDD:275378	2/11 (18%)
		Ig	257..343	CDD:472250	20/88 (23%)
		HRM	351..417	CDD:413313	20/104 (19%)
		GPS	708..752	CDD:460350	13/43 (30%)
		7tm_GPCRs	766..1104	CDD:475119	71/370 (19%)
		TM helix 1	769..793	CDD:410628	8/28 (29%)
		TM helix 2	805..826	CDD:410628	3/20 (15%)
		TM helix 3	836..858	CDD:410628	6/21 (29%)
		TM helix 4	909..925	CDD:410628	4/15 (27%)
		TM helix 5	944..967	CDD:410628	7/31 (23%)
		TM helix 6	1042..1064	CDD:410628	3/21 (14%)
		TM helix 7	1071..1096	CDD:410628	10/43 (23%)