Protein Alignment Mal-A8 and IMA4

DIOPT Version :10

• Sequence 1: NP_610384.1
• Gene: Mal-A8 / 35830
• FlyBaseID: FBgn0033297
• Length: 588
• Species: Drosophila melanogaster
• Sequence 2: NP_012314.1
• Gene: IMA4 / 853235
• SGDID: S000003757
• Length: 589
• Species: Saccharomyces cerevisiae
• Alignment Length: 635
• Identity: 191/635 - (30%)
• Similarity: 286/635 - (45%)
• Gaps: 116/635 - (18%)

  Fly    22 VQSSSSETTKDWWQTAQFYQIYPRSFKDSDGDGIGDLNGITSKLEYLKDLGVTAAWLSPIFKSPM 86
            :.|:..||...||:.|..|||||.|||||:.||.||:.||.|||||:|:||..|.|:||.:.||.
Yeast     3 ISSAHPETEPKWWKEATIYQIYPASFKDSNNDGWGDMKGIASKLEYIKELGTDAIWISPFYDSPQ 67

  Fly    87 VDFGYDISDFFDIQPEYGTLEDFRTLIKRAKELDLKIVLDFVPNHSSNESEWFLKS-VKREKGYE 150
            .|.||||:::..:.|.|||.||...||::..:|.:|.:.|.|.||.|:|.|||.:| ..:.....
Yeast    68 DDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWFKESRSSKTNPKR 132

  Fly   151 DYYVWHDGKVNSTTGKREPPTNWLQYFRGSAWEWNEVRQQYYLHQFAVQQPDLNYRNPLVVEQMK 215
            |::.|...|.....||..||.||..||.||||.::|..|::||..|...|||||:.|    |..:
Yeast   133 DWFFWRPPKGYDAEGKPIPPNNWRSYFGGSAWTFDEKTQEFYLRLFCSTQPDLNWEN----EDCR 193

  Fly   216 RVL-----RYWLNEGVSGFRCDALPPLFEVVPDSDGQFPDEVVSGATEDKEDRDYLTTTYIENQP 275
            :.:     .|||:.||.|||.| :..|:..|    ...||..|.    |:..:..|:..:..|.|
Yeast   194 KAIYESAVGYWLDHGVDGFRID-VGSLYSKV----AGLPDAPVI----DENSKWQLSDPFTMNGP 249

  Fly   276 ETIDMVYQWRTVLDD--HKRIFGGNSSVLLIETYSPAWFTMQFYGNRSTEGAHLPFNFNLITVME 338
            .    ::::...::.  ..|:..|...:.:.|.......|.:.|.:.|.......|||:...|
Yeast   250 R----IHEFHQEMNKFIRNRVKDGREIMTVGEMRHATDETKRLYTSASRHELSELFNFSHTDV-- 308

  Fly   339 QSGLSASNVQEAIDLWLKNMP---------AGRTPNW---VLGNHDKRRAASRYGKEN-----ID 386
              |.|....|..|...||:..         ...|..|   .|.|||:.|:.:|:|.::     |.
Yeast   309 --GTSPKFRQNLIPYELKDWKVALAELFRYVNGTDCWSTIYLENHDQPRSITRFGDDSPKNRVIS 371

  Fly   387 G--MNMLVMILPGVSVTYQGEEIGMTDGEISWEDTVDPWGC---------NSNPNIYEQY----- 435
            |  :::|::.|.|....|||:|:    |||::::    |..         |:...|.|::
Yeast   372 GKLLSVLLVSLSGTLYVYQGQEL----GEINFKN----WPIEKYEDVEVRNNYDAIKEEHGENSK 428

  Fly   436 ------------TRDPERTPFQWT-GGTNAGFTN-GSSTWLPLAADYAT-INVEKELSDDHSHLK 485
                        :||..|||.||: ...||||:. .:..|..|...:.. ||.|.|..|.:|.|.
Yeast   429 EMKRFLEAIALISRDHARTPMQWSREEPNAGFSGPNAKPWFYLNESFREGINAEDESKDPNSVLN 493

  Fly   486 IYKALVALRKSSKTLQNGSTKYQALSED---IFVVQRSLTK---SATIVLVINFGSVAKTVDLSQ 544
            .:|..:..||:.|.:......::.:..|   :|    |.||   :.|:...:||.|
Yeast   494 FWKEALRFRKAHKDITVYGYDFEFIDLDNKKLF----SFTKKYDNKTLFAALNFSS--------- 545

  Fly   545 FDKSRTESLQLLI-RSIDSTKKEGTRY-----DPKALSLDPSEALVLTTE 588
                  :|:...| .:..|.|.|...|     |..:.:|.|.|..:..:|
Yeast   546 ------DSIDFTIPNNSSSFKLEFGNYPRSEVDASSRTLKPWEGRIYISE 589

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mal-A8	NP_610384.1	AmyAc_maltase	30..506	CDD:200467	168/531 (32%)
IMA4	NP_012314.1	AmyAc_SI_OligoGlu_DGase	16..503	CDD:200472	163/515 (32%)
		Malt_amylase_C	514..585	CDD:435493	19/89 (21%)