DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mal-A1 and IMA4

DIOPT Version :9

Sequence 1:	NP_476627.3	Gene:	Mal-A1 / 35824	FlyBaseID:	FBgn0002570	Length:	577	Species:	Drosophila melanogaster
Sequence 2:	NP_012314.1	Gene:	IMA4 / 853235	SGDID:	S000003757	Length:	589	Species:	Saccharomyces cerevisiae

Alignment Length:	585	Identity:	177/585 - (30%)
Similarity:	271/585 - (46%)	Gaps:	98/585 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 EWWESGNYYQIYPRSFRDSDGDGIGDLNGVTEKLQYLKDIGFTGTWLSPIFKSPMVDFGYDISDF 85
            :||:....|||||.||:||:.||.||:.|:..||:|:|::|....|:||.:.||..|.||||:::
Yeast    13 KWWKEATIYQIYPASFKDSNNDGWGDMKGIASKLEYIKELGTDAIWISPFYDSPQDDMGYDIANY 77

  Fly    86 YQIHPEYGTMEDFERMIAKAKEVGIKIILDFVPNHSSTENEWF--TKSVDSDPVYKDFYIWHDGK 148
            .::.|.|||.||...:|.|..::|:|.|.|.|.||.|:|:|||  ::|..::| .:|::.|...|
Yeast    78 EKVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWFKESRSSKTNP-KRDWFFWRPPK 141

  Fly   149 INNETGEREPPSNWNSEFRYSAWEWNEVRQQYYLHQFAIQQADLNYRNPAVVNEM-KNVIRFWLG 212
            ..:..|:..||:||.|.|..|||.::|..|::||..|...|.|||:.|......: ::.:.:||.
Yeast   142 GYDAEGKPIPPNNWRSYFGGSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYWLD 206

  Fly   213 KGVSGFRIDAVPYLFEVDLDRYNQYPDEPL--TNDSVNCPDPDDHCYTQHIYTQDMPETIDMVYQ 275
            .||.||||| |..|:    .:....||.|:  .|......||         :|.:.|.    :::
Yeast   207 HGVDGFRID-VGSLY----SKVAGLPDAPVIDENSKWQLSDP---------FTMNGPR----IHE 253

  Fly   276 WRELVDEFHVENGGDKRLLMT--EAYTSFENIMTYYGNGVRNGSHIPFNFDFLTSINNASKAGEY 338
            :.:.:::|......|.|.:||  |...:.:.....|.:..|:.....|||.. |.:..:.|..:.
Yeast   254 FHQEMNKFIRNRVKDGREIMTVGEMRHATDETKRLYTSASRHELSELFNFSH-TDVGTSPKFRQN 317

  Fly   339 V--EHIKKWMDAMPEGV-YAN----W---VLGNHDNKRVASRFG-------VQRTDLINILLQTL 386
            :  ..:|.|..|:.|.. |.|    |   .|.|||..|..:|||       |....|:::||.:|
Yeast   318 LIPYELKDWKVALAELFRYVNGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLVSL 382

  Fly   387 PGHAVTYNGEELGMTDVWISW--EDTVDPNACNSDPDNYYA---------------------RSR 428
            .|....|.|:|||..: :.:|  |...|....|    ||.|                     .||
Yeast   383 SGTLYVYQGQELGEIN-FKNWPIEKYEDVEVRN----NYDAIKEEHGENSKEMKRFLEAIALISR 442

  Fly   429 DPARSPYQWDASS-KAGFTSAD-HTWLPVADDYKTN-NALQQLRAPRSHLQIFKKLVRVRKEPSF 490
            |.||:|.||.... .|||:..: ..|..:.:.::.. ||..:.:.|.|.|..:|:.:|.||
Yeast   443 DHARTPMQWSREEPNAGFSGPNAKPWFYLNESFREGINAEDESKDPNSVLNFWKEALRFRK---- 503

  Fly   491 RQGELNIQAIDDDVIIYSRQKTGSDLYVIVLNLGSTSKTLDLTKYYELGTQAEVITTSLSSQYID 555
                     ...|:.:|     |.|...|.|:   ..|....||.|:..|....:  :.||..||
Yeast   504 ---------AHKDITVY-----GYDFEFIDLD---NKKLFSFTKKYDNKTLFAAL--NFSSDSID 549

Fly 556 555
Yeast 550 549

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mal-A1	NP_476627.3	AmyAc_maltase	21..496	CDD:200467	162/524 (31%)
Mal-A1	NP_476627.3	Alpha-amylase	45..404	CDD:278554	121/382 (32%)
IMA4	NP_012314.1	AmyAc_SI_OligoGlu_DGase	16..503	CDD:200472	158/511 (31%)
IMA4	NP_012314.1	Malt_amylase_C	514..585	CDD:406946	12/41 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	192	1.000	Domainoid score	I624
eggNOG	1	0.900	-	-		E1_COG0366
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H113646
Inparanoid	1	1.050	257	1.000	Inparanoid score	I641
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000282
OrthoInspector	1	1.000	-	-		mtm9159
orthoMCL	1	0.900	-	-		OOG6_100402
Panther	1	1.100	-	-	O	PTHR10357
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X202
TreeFam	0	0.000	Not matched by this tool.
	11	10.860