Protein Alignment Mlh1 and MLH1

DIOPT Version :10

• Sequence 1: NP_477022.1
• Gene: Mlh1 / 35796
• FlyBaseID: FBgn0011659
• Length: 664
• Species: Drosophila melanogaster
• Sequence 2: NP_000240.1
• Gene: MLH1 / 4292
• HGNCID: 7127
• Length: 756
• Species: Homo sapiens
• Alignment Length: 751
• Identity: 349/751 - (46%)
• Similarity: 477/751 - (63%)
• Gaps: 94/751 - (12%)

  Fly     8 GVIRKLDEVVVNRIAAGEIIQRPANALKELLENSLDAQSTHIQVQVKAGGLKLLQIQDNGTGIRR 72
            ||||:|||.|||||||||:|||||||:||::||.|||:||.|||.||.|||||:||||||||||:
Human     6 GVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRK 70

  Fly    73 EDLAIVCERFTTSKLTRFEDLSQIATFGFRGEALASISHVAHLSIQTKTAKEKCGYKATYADGKL 137
            |||.|||||||||||..||||:.|:|:|||||||||||||||::|.||||..||.|:|:|:||||
Human    71 EDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKL 135

  Fly   138 QGQPKPCAGNQGTIICIEDLFYNMPQRRQALRSPAEEFQRLSEVLARYAVHNPRVGFTLRKQGDA 202
            :..||||||||||.|.:||||||:..||:||::|:||:.::.||:.||:|||..:.|:::|||:.
Human   136 KAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGET 200

  Fly   203 QPALRTPVASSRSENIRIIYGAAISKELLEFSHRDEVYKFEAECLITQVNYSAKKCQMLLFINQR 267
            ...:||...:|..:|||.|:|.|:|:||:|....|:...|:....|:..|||.|||..|||||.|
Human   201 VADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHR 265

  Fly   268 LVESTALRTSVDSIYATYLPRGHHPFVYMSLTLPPQNLDVNVHPTKHEVHFLYQEEIVDSIKQQV 332
            |||||:||.:::::||.|||:..|||:|:||.:.|||:||||||||||||||::|.|::.::|.:
Human   266 LVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHI 330

  Fly   333 EARLLGSNATRTFYKQLRLPG-----------APDLDETQLADKTQRIYPKEMVRTDSTEQKLDK 386
            |::|||||::|.::.|..|||           ...|..:..:..:.::|..:||||||.|||||.
Human   331 ESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDA 395

  Fly   387 FLAPLVKS----------------DSGVSSSSSQEASRLP------------------------- 410
            ||.||.|.                .||.:....:|...||
Human   396 FLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSE 460

  Fly   411 ----------------------EESFR--VTAA--KKSREVRLSSVLDMRKRVERQCSVQLRSTL 449
                                  |:..|  :|||  .:.|.:.|:|||.:::.:..|....||..|
Human   461 KRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREML 525

  Fly   450 KNLVYVGCVDERRALFQHETRLYMCNTRSFSEELFYQRMIYEFQNCSEITISPPLPLKELLILSL 514
            .|..:||||:.:.||.||:|:||:.||...|||||||.:||:|.|...:.:|.|.||.:|.:|:|
Human   526 HNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLAL 590

  Fly   515 ESEAAGWTPEDGDKAELADGAADILLKKAPIMREYFGLRISEDGMLESLPSLLHQHRPCVAHLPV 579
            :|..:|||.|||.|..||:...:.|.|||.::.:||.|.|.|:|.|..||.|:..:.|.:..||:
Human   591 DSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPI 655

  Fly   580 YLLRLATEVDWEQETRCFETFCRETARFYA---QLDWREGATAGFS-----------RWTMEHVL 630
            ::|||||||:|::|..|||:..:|.|.||:   |....|...:|..           :||:||::
Human   656 FILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIV 720

  Fly   631 FPAFKKYLLPPPRIKD--QIYELTNLPTLYKVFERC 664
            :.|.:.::|||....:  .|.:|.|||.||||||||
Human   721 YKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC 756

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mlh1	NP_477022.1	mutl	8..317	CDD:273155	195/308 (63%)
		Mlh1_C	426..664	CDD:465109	105/253 (42%)
MLH1	NP_000240.1	mutl	6..315	CDD:273155	195/308 (63%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	355..378		1/22 (5%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	400..491		9/90 (10%)
		Interaction with EXO1. /evidence=ECO:0000269|PubMed:11427529, ECO:0000269|PubMed:11429708, ECO:0000269|PubMed:12414623, ECO:0000269|PubMed:14676842, ECO:0000269|PubMed:22753075	410..650		76/239 (32%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:29175432	471..474		0/2 (0%)
		Mlh1_C	502..756	CDD:465109	105/253 (42%)