DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Asap and Appl1

DIOPT Version :9

Sequence 1:	NP_001014504.1	Gene:	Asap / 35783	FlyBaseID:	FBgn0050372	Length:	1155	Species:	Drosophila melanogaster
Sequence 2:	NP_660256.1	Gene:	Appl1 / 72993	MGIID:	1920243	Length:	707	Species:	Mus musculus

Alignment Length:	811	Identity:	168/811 - (20%)
Similarity:	296/811 - (36%)	Gaps:	212/811 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 VEETRSDYSSPTTSTFASRMPDCRHTIGVLEERLEFDREGLTKLKKAVKAIHNSGN-----THVD 71
            :|||..|  ||.|          |..:||.||........:.:|.:|:..|:::.|     ||:.
Mouse     9 IEETLED--SPQT----------RSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLT 61

  Fly    72 NEMFMVRALER--LGGKVIEQDEPDIGAAFLKFSVVTKELSALMKTLMQNINNIVMFPVDSMLKS 134
            :::......:|  |||     |:..:.:...:||.|..|||:....|...:.:.:|||:....:.
Mouse    62 SKLLKEYEKQRFPLGG-----DDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPISQFKER 121

  Fly   135 ELRGVKGDMKRPFDKAAKDYEA---KFIKIEKEKKAQAKEAGMVRTEIDAAVVAEEMEKERRLYQ 196
            :|:.:. .:|..|..|:.|::|   ::.::.|:     :|...|:.|     |.|::...|:...
Mouse   122 DLKEIL-TLKEVFQIASNDHDAAINRYSRLSKK-----RENDKVKYE-----VTEDVYTSRKKQH 175

  Fly   197 LQTCEYLLKYKDIKTKTGIELLQHLIEYYHALSNYFKDGLQTIEHFGTYIGDLSEKLHEIKQKQD 261
            .....|......::.|..|.||:.|:.|..|..::||.|.:.:.      |.|.|.|..|.....
Mouse   176 QTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLN------GQLEEFLANIGTSVQ 234

  Fly   262 EDRRSL-LDLRTVLRSTPDFERVDN-----------VPSSESRSGGAGY-------SLHQLQGDK 307
            ..||.: .|:.|:.::..|.|...:           .|.:.:.:..|||       .|.....|:
Mouse   235 NVRREMDGDVETMQQTIEDLEVASDPLYLPDPDPTKFPINRNLTRKAGYLNARNKTGLVSSTWDR 299

  Fly   308 HHGVTRQGHLLKKSEGKVRRVWQKRRCRVTSDGF-LDIFHADESKPPTRVNLLTCQIKPVP-DDK 370
            ....|:.|:|:.::.|.|            :.|. :||.:              |.:..|. :|:
Mouse   300 QFYFTQGGNLMSQARGDV------------AGGLAMDIDN--------------CSVMAVDCEDR 338

  Fly   371 R-GFDLISYN--RPYHFQAEDEGDQKAWMAVLVNCK---------EKALTKAFQHANPQMSPSLV 423
            | .|.:.|::  :....|||.:.|.:.|:..:.|..         |:...:..|.|...::||..
Mouse   339 RYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPS 403

  Fly   424 ELQKTVIRYVQLLPGND------RCCDCGSRNDVTWISLNFGILVCIQCSGVHRDLGVHHSRIQS 482
            ..|    |:..|.||..      |....||                         ||...:.:.:
Mouse   404 FQQ----RHESLRPGGQSRPPTARTSSSGS-------------------------LGSESTNLAA 439

  Fly   483 LTLDNLTTANLLI---------------ARAMGNS--TLNDIMEAKLGRGKLQHESSMEERYDFI 530
            |:||:|...:..|               |:|.|..  ..|...|:. |..|.:.|.|:..:...:
Mouse   440 LSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESG-GSTKSETEDSILHQLFIV 503

  Fly   531 R--------------AKYVAKRYVMRTCSDDNDLRCDLEQAVVNADMSQLLQVWAEGADLTCCLP 581
            |              ..|...|.::...:..|..|......:|..|..:|:....:...||..||
Mouse   504 RFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLP 568

  Fly   582 -----SSDAGETALHLAVLREMG----STLHIVDFLIQNMPPKGLNKATNPAGLLDVTGKNTALH 637
                 ::......|...|||..|    |.|..|.::.:: ..:| .|..:..||    .|..|||
Mouse   569 CVVLYATHQENKRLFGFVLRTSGGRSESNLSSVCYIFES-NNEG-EKICDSVGL----AKQIALH 627

  Fly   638 LCALHDRRECMKLLLRSGADYELKNSQNKTALDIAKEMGHNSCRELIECAIKREKSAFDHINTDW 702
              |..|||...|       ..|::..:.|...:::|:.           .|:::......:....
Mouse   628 --AELDRRASEK-------QKEIERVKEKQQKELSKQK-----------QIEKDLEEQSRLIAAS 672

  Fly   703 NLPNEDG-------STDFSDDETVIDERKSR 726
            :.||:.|       |:..|::..:.:|.|.|
Mouse   673 SRPNQAGSEGQLVLSSSQSEESDLGEEGKKR 703

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Asap	NP_001014504.1	BAR_ASAPs	35..249	CDD:153288	51/223 (23%)
		PH_ASAP	302..410	CDD:270071	22/121 (18%)
		ArfGap_ASAP	425..541	CDD:350063	27/152 (18%)
		Ank_2	554..662	CDD:403870	29/116 (25%)
		ANK repeat	586..622	CDD:293786	10/39 (26%)
		ANK repeat	624..662	CDD:293786	12/37 (32%)
		Ank_2	636..>693	CDD:403870	11/56 (20%)
		SH3_ASAP	1095..1149	CDD:212755
Appl1	NP_660256.1	Required for RAB5A binding. /evidence=ECO:0000250	1..428		105/482 (22%)
		BAR_APPL1	20..234	CDD:153315	55/235 (23%)
		BAR-PH_APPL	252..376	CDD:270067	28/149 (19%)
		PH	280..375	CDD:278594	25/120 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	397..433		12/64 (19%)
		F&H. /evidence=ECO:0000250	403..414		3/14 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	466..490		6/24 (25%)
		PTB_APPL	489..624	CDD:269980	28/140 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	636..707		13/86 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5347
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810