DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Asap and Git1

DIOPT Version :9

Sequence 1:	NP_001014504.1	Gene:	Asap / 35783	FlyBaseID:	FBgn0050372	Length:	1155	Species:	Drosophila melanogaster
Sequence 2:	NP_001004144.1	Gene:	Git1 / 216963	MGIID:	1927140	Length:	770	Species:	Mus musculus

Alignment Length:	726	Identity:	150/726 - (20%)
Similarity:	253/726 - (34%)	Gaps:	194/726 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly   437 PGNDRCCDCGSRNDVTWISLNFGILVCIQCSGVHRDLGVHHSRIQSLTLDNLTTANLLIARAMGN 501
            |..:.|.|| |..|..|.|::.|:|||.:|..|||.||.|.|.::.|.........|.:...:.:
Mouse     6 PRAEVCADC-SAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLAS 69

  Fly   502 STLNDIMEAKL--------GRGKLQHESSMEE-RYDFIRAKYVAKRYVMR-TCSDDNDLRCDLEQ 556
            :..|.|.|..|        ||.|...:..:.. :.:||||||....:|.: .|.||:
Mouse    70 NGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDD-------- 126

  Fly   557 AVVNADMSQLLQVWAEGADLTCCLPSSDAGETALHLAVLREMGSTLHIVDFLIQNMPPKGLNKAT 621
            .|...|:|:.|.......:|          ||.|.|..|....:..|         |.||
Mouse   127 GVTAKDLSKQLHSSVRTGNL----------ETCLRLLSLGAQANFFH---------PEKG----- 167

  Fly   622 NPAGLLDVTGKNTALHLCALHDRRECMKLLLRSGADYELKNSQNKTALDIAKEMGHNSCRE-LIE 685
                       .|.||:.|...:....:||:..|||....:...:|.:|.|::.||:...| |:|
Mouse   168 -----------TTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVE 221

  Fly   686 CAIK-REKSAF-------DHINTDWNLPN-----------------------------------E 707
            |..: .::.||       ||.|..:.:|.                                   |
Mouse   222 CQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFE 286

  Fly   708 DGSTDFSDDETVIDERKSRSRPPSFAGGDSPVLRSRSSTCDSIQSSSSPIANCPSRQFTLPSGLP 772
            :.:.|..|:   :|.|::          |:..|.:::.:....:.|:.|.         ||.. |
Mouse   287 ELAMDVYDE---VDRREN----------DAVWLATQNHSTLVTERSAVPF---------LPVN-P 328

  Fly   773 SY--THSAGTSPKQHISVGQY----LGSATNVGGNGPGNGGSSPSSASSQSVRAARNSLNMQSDL 831
            .|  |.:.|.......:..::    :...:.......|...|||:.....|.| :::.|:.|.|.
Mouse   329 EYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSAR-SQSELDDQHDY 392

  Fly   832 GGHVT------------GARKSTSTANMNS---------------LKKRTAPAPPPGTLGSASSS 869
            ....:            ||.::....:|:|               |||..|.:..........:|
Mouse   393 DSVASDEDTDQEPLPSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNS 457

  Fly   870 SFYGTLPHPPRHSQNFDASDIRAINHKNQSLDVAYGTLPHLRSVESSPRGGGG------------ 922
            |....|....|......|.:::.   :.....|...:||..|: |.:..|.||
Mouse   458 SLSDELRRLQREIHKLQAENLQL---RQPPGPVPPPSLPSERA-EHTLMGPGGSTHRRDRQAFSM 518

  Fly   923 YGYGVSQDPGGSGNGSNNSLMPAMTTFGHKRSPSGESLNRNIHL-AGAKLV--------LPPTGE 978
            |..|.:..|.|...|  :.|...:..| |......::: .::|: ||...:        :|.|..
Mouse   519 YEPGSALKPFGGTPG--DELATRLQPF-HSTELEDDAI-YSVHVPAGLYRIRKGVSASSVPFTPS 579

  Fly   979 LPTLKHVDKSALTRPKIPPPGPPSEREISNGQSNESISSMDEGPVAPPRKLVNQSANFPDYESWH 1043
            .|.|....:.:....|:...|..::.:..|.||.:.:..: ||     ::.:..|..    :..|
Mouse   580 SPLLSCSQEGSRHASKLSRHGSGADSDYENTQSGDPLLGL-EG-----KRFLELSKE----DELH 634

  Fly  1044 TDMDSSGGGLD 1054
            .:::|..|.||
Mouse   635 PELESLDGDLD 645

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Asap	NP_001014504.1	BAR_ASAPs	35..249	CDD:153288
		PH_ASAP	302..410	CDD:270071
		ArfGap_ASAP	425..541	CDD:350063	35/112 (31%)
		Ank_2	554..662	CDD:403870	23/107 (21%)
		ANK repeat	586..622	CDD:293786	9/35 (26%)
		ANK repeat	624..662	CDD:293786	9/37 (24%)
		Ank_2	636..>693	CDD:403870	17/58 (29%)
		SH3_ASAP	1095..1149	CDD:212755
Git1	NP_001004144.1	Interaction with gamma-tubulin and localization to the centrosome. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	1..124		37/118 (31%)
		ArfGap_GIT1	1..111	CDD:350071	33/105 (31%)
		ANK 1	132..161		9/38 (24%)
		Ank_2	137..229	CDD:372319	29/126 (23%)
		ANK repeat	137..165	CDD:293786	8/46 (17%)
		ANK 2	166..195		11/44 (25%)
		ANK repeat	167..197	CDD:293786	10/45 (22%)
		ANK 3	199..228		9/28 (32%)
		Interaction with PCLO. /evidence=ECO:0000250\|UniProtKB:Q9Z272	245..374		20/151 (13%)
		Interaction with PTK2/FAK1. /evidence=ECO:0000250\|UniProtKB:Q9Z272	253..424		28/194 (14%)
		Interaction with ARHGEF7. /evidence=ECO:0000250\|UniProtKB:Q9Z272	254..376		19/144 (13%)
		GIT_SHD	<281..301	CDD:369921	5/22 (23%)
		GIT_SHD	337..365	CDD:369921	1/27 (4%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	363..425		13/62 (21%)
		Interaction with NCK2 and GRIN3A. /evidence=ECO:0000250\|UniProtKB:Q9Z272	375..596		41/229 (18%)
		Required for localization at synapses. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	375..596		41/229 (18%)
		GIT_CC	418..482	CDD:374629	11/66 (17%)
		Interaction with MAPK1. /evidence=ECO:0000269\|PubMed:15923189	420..475		10/54 (19%)
		Interaction with IKBKG. /evidence=ECO:0000250\|UniProtKB:Q9Y2X7	429..629		39/213 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	578..615		7/36 (19%)
		Interaction with PXN and TGFB1I1. /evidence=ECO:0000250\|UniProtKB:Q9Z272	646..770		150/726 (21%)
		GIT1_C	649..763	CDD:371961
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5347
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810