Protein Alignment nito and Spen

DIOPT Version :10

• Sequence 1: NP_610339.1
• Gene: nito / 35756
• FlyBaseID: FBgn0027548
• Length: 793
• Species: Drosophila melanogaster
• Sequence 2: XP_006539132.1
• Gene: Spen / 56381
• MGIID: 1891706
• Length: 3644
• Species: Mus musculus
• Alignment Length: 759
• Identity: 174/759 - (22%)
• Similarity: 251/759 - (33%)
• Gaps: 310/759 - (40%)

  Fly     4 HRDGAGADRITIKI------HNMKRSASRSPGPASRSSLSRNSRSMGRGYDNGSGVPGDSPERLS 62
            ||| ...|.||.::      .:.:.|.||||    .||.|||                .||:||:
Mouse   216 HRD-IYRDDITREVRGRRPERSYQHSRSRSP----HSSQSRN----------------QSPQRLA 259

  Fly    63 PERMRRRLDRSPSRYGSPHR----------------------------EPYMRGPPPAERPA--- 96
            .:..|.  .||||..||..|                            :...|....|..||
Mouse   260 SQASRP--TRSPSGSGSRSRSSSSDSISSSSSSSSNTDSSDSSSTASDDSPARSVQSAAVPAPTS 322

  Fly    97 ---------------GYKVLCVSALPPKAPDDFIEETLYREYKKFGDF-SVRLAHDLDERVAYVC 145
                           |.|   |..||.::.|..:::.|:.|:||||.. ||::....:||...|.
Mouse   323 QLLSSLEKDEPRKSFGIK---VQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGASEERYGLVF 384

  Fly   146 FRTPEDAREA-KHHKPRLIIYDKMAIVEPVYKST-TRPEYRPRGHSMSPPDYERYHYSRSPMGQG 208
            ||..||..:| ...|.:|....::.:...|...| :..|:|
Mouse   385 FRQQEDQEKALTASKGKLFFGMQIEVTAWVGPETESENEFR------------------------ 425

  Fly   209 VPLDHRRPPIDPYDRYGPPHMHPHAVHPRDYRPLHHDYPHPPRGPPLHRGGHPHHLHGHAPPHQY 273
             |||.|   ||.:             ||:
Mouse   426 -PLDER---IDEF-------------HPK------------------------------------ 437

  Fly   274 APMRPLAPRPHAPYEKPESKKDKFPNYLHHVQPEDDPLSTRTLFAGNLEVTIADDELRRIFGKYG 338
                                                  :|||||.||||.|....:||.||.::|
Mouse   438 --------------------------------------ATRTLFIGNLEKTTTYHDLRNIFQRFG 464

  Fly   339 VVDDIDIKR----PPPGTGNAFAFVRYQNLDMAHRAKIELSGQYIGKFQCKIGYGKVTPATRMWI 399
            .:.|||||:    |      .:||::|.::....:|..::.|:|:|..:.|:|:||..|...:|:
Mouse   465 EIVDIDIKKVNGVP------QYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWL 523

  Fly   400 GGLGAWTSVTQLEREFDRFGAIKKIEYQKGEPYAYIQYETVEAATAAVKEMRGFPLGGPERRLRT 464
            .||.:..|...|.|.|.|:|.:.|:.:.:.:..|.:.|..:|.|.|||||.:|..:||  .:::.
Mouse   524 DGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYSEIEDAQAAVKETKGRKIGG--NKIKV 586

  Fly   465 DFAELPGATPAAPFKSSKPPYDESALEY----------RRPEYDPYYEESAAYAPRGGY------ 513
            |||.                 .||.|.:          .|..|:...|..|....:..:
Mouse   587 DFAN-----------------RESQLAFYHCMEKSGQDMRDFYEMLTERRAGQMAQSKHEDWSAD 634

  Fly   514 --SPYPPRGGYRGRGGYRGRGRGMYHYHNDVHRPPHPGSLAGSSSSVPPPGGVE--DEWRRPPGE 574
              ||:..|...||...|.....    |:.:|..   ||:....|....|..|.|  .||....||
Mouse   635 AQSPHKCREERRGSYEYSQERT----YYENVRT---PGTYPEDSRRDYPARGREFYSEWETYQGE 692

  Fly   575 SYD---------------------------------------RGARSSSREPGVERSRSRSPLKR 600
            .||                                       ...|...|.| :|||:|...|:|
Mouse   693 YYDSRYYDEPREYREYRSDPYEQDIREYSYRQRERERERERFESDRDHERRP-IERSQSPVHLRR 756

  Fly   601 ARSPG----------SDSDTSTRRNDALASASTVPDVARKCSTV 634
            .:|||          |||:....|..:..|.|        ||:|
Mouse   757 PQSPGVSPAHSERLPSDSERRLYRRSSERSGS--------CSSV 792

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nito	NP_610339.1	RRM1_Spen	98..175	CDD:409749	23/78 (29%)
		RRM2_Spen	312..390	CDD:240755	30/81 (37%)
		RRM3_Spen	397..468	CDD:409750	24/70 (34%)
		SPOC_RBM15-like	634..790	CDD:439207	1/1 (100%)
Spen	XP_006539132.1	RRM1_SHARP	7..81	CDD:409784
		U2AF_lg	128..596	CDD:273727	128/545 (23%)
		RRM2_SHARP	338..411	CDD:409785	24/75 (32%)
		RRM3_SHARP	438..511	CDD:409786	29/78 (37%)
		RRM4_SHARP	512..588	CDD:409787	25/77 (32%)
		PTZ00121	<635..1272	CDD:173412	41/174 (24%)
		PHA03247	<1629..2063	CDD:223021
		PHA03247	<2122..2642	CDD:223021
		Collagen_mid	<2641..>2719	CDD:435053
		PHA03247	<2949..3474	CDD:223021
		SPOC_SHARP	3484..3644	CDD:439206