Protein Alignment drosha and DCL4

DIOPT Version :10

• Sequence 1: NP_477436.1
• Gene: drosha / 35747
• FlyBaseID: FBgn0026722
• Length: 1327
• Species: Drosophila melanogaster
• Sequence 2: NP_197532.3
• Gene: DCL4 / 832154
• AraportID: AT5G20320
• Length: 1702
• Species: Arabidopsis thaliana
• Alignment Length: 1579
• Identity: 296/1579 - (18%)
• Similarity: 481/1579 - (30%)
• Gaps: 645/1579 - (40%)

  Fly   152 YSSGRRYEQRHDQEHRQIQDSR--------YAHEPRH---------------GHYAHRQAKGSQH 193
            :..|:|..:.|.:..|:|..:.        ..|..:|               .|:|.:|   |.|
plant   201 HCGGKRIVKSHSEWEREIAANEVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQQ---SNH 262

  Fly   194 GY-------YGSAARNQVSDDYSPRGHHERERNETLEKTR-----AKPKVETERDRLLRQWCSNF 246
            .|       |                     ::|:|::.|     |.|.|..          .:|
plant   263 PYAEIMKVFY---------------------KSESLQRPRIFGMTASPVVGK----------GSF 296

  Fly   247 CEKPEDYVKKMNALS----------EADAPVESWVRSSPAELYYERTKSENEVRGRARLQKLCTL 301
              :.|:..|.:|:|.          |::..::.:|.|...::||.|:...:..:...|.:.:.
plant   297 --QSENLSKSINSLENLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENML-- 357

  Fly   302 FDEELLQRA-----------------------KRVREKLPVYV---------------------- 321
              |::.||.                       ||..:.| :|.
plant   358 --EDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNL-IYTLLNLGLWGAIQAAKIQLNSDHN 419

  Fly   322 ----PPPRKARRRVCKH--KHKSEACSSSSSSDDDSDE-----------------------DAFK 357
                |..:..:.::|..  ...:||.||..:.|:::.:                       ..|:
plant   420 VQDEPVGKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFR 484

  Fly   358 IEQDC-CMEELSRKVQHPQRVHADLWHNDAGEMNDGPLCRCSAKSRRIGIRHGIYPGETGYKLCD 421
            :|... |:..::|.|  ..|..:.:       :|:..|.|.......:|:..|:      ..:..
plant   485 LEPHMKCIIFVNRIV--TARTLSCI-------LNNLELLRSWKSDFLVGLSSGL------KSMSR 534

  Fly   422 PNSNNAGKLFHYRISISPPTNFLTKTPT------------IIKHD-------------------- 454
            .:.....|.|.     |...|.|..|..            :|::|
plant   535 RSMETILKRFQ-----SKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQS 594

  Fly   455 EHEFLFEG-----FSLLSHVRLSDLPVCKVIRFNIEYTIEYEEEKMP----ENFTIHE------- 503
            |:.||.:.     ..|:.:.::::      .|.|:|.|....||..|    |.:.:||
plant   595 EYAFLVDSGNEKEMDLIENFKVNE------DRMNLEITYRSSEETCPRLDEELYKVHETGACISG 653

  Fly   504 ---LDIFFKYLFHELLELVDFNLMPNLPSGNVEESCPAFHFFP---------RFVRDLPDNG--- 553
               :.:.:||             ...||.....:..|.|.|.|         |..  ||.|.
plant   654 GSSISLLYKY-------------CSRLPHDEFFQPKPEFQFKPVDEFGGTICRIT--LPANAPIS 703

  Fly   554 ---KEVLAMVEVLR-------------------YLLDNSAQLVERQQLLHLNQISQSEWQNYVDF 596
               ..:|...|..:                   :||.:|...:|       :::|..|:.  .|.
plant   704 EIESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIE-------DELSDDEFD--FDN 759

  Fly   597 IKGMLVTKPGYKPCS------LRVDQLDRNNSD-LPECVDRETGISHPAIVHFGICHPQLSYAGN 654
            |||        :.||      :||..|.:...| ...||:     .|...:.| :.||
plant   760 IKG--------EGCSRGDLYEMRVPVLFKQKWDPSTSCVN-----LHSYYIMF-VPHP------- 803

  Fly   655 PEYQKAWREYVKYRHLM-----------------ANMSKPSFK-----------DKRKLEEKEQR 691
                 |.|.|.|:...|                 |:....|.|           |:.:|.|..|.
plant   804 -----ADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPSGVTEFDNDEIRLAELFQE 863

  Fly   692 LQ-----------------EMRTQGRMKRN---------------------ITV--AISSEGFYR 716
            :.                 |::...|..::                     :|:  .:||..|..
plant   864 IALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKT 928

  Fly   717 TGIMCDVVQHAMLIPVLTGHLRFHK---SLDLLEESIGY-RFKNRYLLQLALTH----------- 766
            ..::.:.:     .|....||:...   ::|.::.|:.: .:..::.....:.|
plant   929 PSVLVEDI-----FPPSGSHLKLANGCWNIDDVKNSLVFTTYSKQFYFVADICHGRNGFSPVKES 988

  Fly   767 --PSYKEN----YGTNPDHARNSLTN----CGIRQ-----------------------PEYGDRK 798
              .|:.|:    ||....|....|..    |.:|.                       ||....|
plant   989 STKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRNLLHNRMQTNLEPQELDEYFIEIPPELSHLK 1053

  Fly   799 IHYMNTR-KRGINTLVSIMSRF----------------------------------GKEHETVSN 828
            |..::.. ...::.|.|||.|.                                  .|.||.:|
plant  1054 IKGLSKDIGSSLSLLPSIMHRMENLLVAIELKHVLSASIPEIAEVSGHRVLEALTTEKCHERLS- 1117

  Fly   829 ITHNERLEFLGDAVVEFLSSIHLFFMFPELEEGGLATYRAAIVQNQHLALLAKKLQLE------- 886
               .||||.||||.::|..|.|||.....|:||.|...|:.:|.|.:|..||.|..|:
plant  1118 ---LERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQA 1179

  Fly   887 ----EFMLYAH----------------------------GSDLC---HELELRHAMANCFEALMG 916
                :|..:.|                            |...|   |....:..:|:..|||:|
plant  1180 LDPTQFFAFGHPCRVTCDEVASKEVHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVG 1244

  Fly   917 ALLLDGGIKVADEVFTDALFRQDEKLLS-IWKNLPEHPLQEQEPLGDRSCIDSYRVL-----KEL 975
            |.|:|.|.|.|            .|.|. |..|:....||.|:     :||.|.|.|     ..|
plant  1245 AFLVDSGFKGA------------VKFLKWIGVNVDFESLQVQD-----ACIASRRYLPLTTRNNL 1292

  Fly   976 TKFEDSIGIKFKHIRLLARAFTDRSIGFTHLTLGSNQRLEFLGDTVLQLICSEYLYRHFPEHHEG 1040
            ...|:.:..||.|..||.:||...|  :.....|..||||||||.||..:.:.|.:..||:...|
plant  1293 ETLENQLDYKFLHKGLLVQAFIHPS--YNRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPG 1355

  Fly  1041 HLSLLRSSLVNNRTQAVVCDDLGMPKY----AVY-------------ANPKADLKT------KDR 1082
            .|:.|||..|||...|.|.....:.::    ::|             ::|.|..::      |..
plant  1356 QLTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVL 1420

  Fly  1083 ADLLEAFLGALYVDKGLLYCEQFCHVCLFPRLQLFIMNQDWN-----------------DPKSKL 1130
            .||:|:.||||::|.|                  |.:|..|.                 .|..:|
plant  1421 GDLVESCLGALFLDCG------------------FNLNHVWTMMLSFLDPVKNLSNLQISPIKEL 1467

  Fly  1131 QQCCLTL---RTMDGGEPDIPYYKVVEASGPTNTRVYKVAVYFRSKRLATSSGSSIQQAEMNAAK 1192
            .:.|.:.   |.:...:.|..:  .||.....|.....|:...|:||..|...:.:....:
plant  1468 IELCQSYKWDREISATKKDGAF--TVELKVTKNGCCLTVSATGRNKREGTKKAAQLMITNL---- 1526

  Fly  1193 QALENSRDLFPQLDHQKRVIAKSIKKQTGNE----------LD-----------NDSDRQHQEEK 1236
            :|.||.....|..|..|..|....|....||          ||           .:|:|. ....
plant  1527 KAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVVDLVGLDVENLNILETFGGNSERS-SSYV 1590

  Fly  1237 IKRPKYATPLQDESHLPKQ 1255
            |:|.....|.:.|..||::
plant  1591 IRRGLPQAPSKTEDRLPQK 1609

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
drosha	NP_477436.1	RIBOc	826..942	CDD:197778	42/157 (27%)
		Rnc	973..1196	CDD:440336	64/265 (24%)
DCL4	NP_197532.3	DEXHc_dicer	122..321	CDD:350792	25/155 (16%)
		SF2_C_dicer	466..600	CDD:350189	21/153 (14%)
		Dicer_dimer	656..745	CDD:460900	17/103 (17%)
		PAZ	937..1080	CDD:198017	23/142 (16%)
		RIBOc	1104..1261	CDD:238333	47/172 (27%)
		Rnc	1289..1530	CDD:440336	64/266 (24%)
		DSRM_DCL_plant	1627..1697	CDD:380698