Protein Alignment drosha and Dcr-1

DIOPT Version :10

• Sequence 1: NP_477436.1
• Gene: drosha / 35747
• FlyBaseID: FBgn0026722
• Length: 1327
• Species: Drosophila melanogaster
• Sequence 2: NP_524453.1
• Gene: Dcr-1 / 42693
• FlyBaseID: FBgn0039016
• Length: 2249
• Species: Drosophila melanogaster
• Alignment Length: 1218
• Identity: 232/1218 - (19%)
• Similarity: 383/1218 - (31%)
• Gaps: 460/1218 - (37%)

  Fly   201 RNQVSD-DYS-PRGHHERERNETLEKTR-AKPKVETERDRLLRQWCSNFCEKPEDYVKKMNALSE 262
            |.|:.| |:. |........:|.|:|:| :|.|...:.|.:..:..::..:||.....:::..|:
  Fly  1286 RQQIEDEDFEWPMLDFGWSLSEVLKKSRESKQKESLKDDTINGKDLADVEKKPTSEETQLDKDSK 1350

  Fly   263 ADAPVESWVRSSPAELYYERTKSENEVRGRARLQKLCTLFDEELLQRAKRVREKLPVYVPPPRKA 327
            .|.     |..|..||..|                     .||.||.|....|
  Fly  1351 DDK-----VEKSAIELIIE---------------------GEEKLQEADDFIE------------ 1377

  Fly   328 RRRVCKHKHKSEACSSSSSSDDDSDEDAFKIEQDCCMEELSRKV----QHPQRVHADLWHNDAGE 388
               :....:......:|.:.:||.::|||.      :..|...|    |..:......|....||
  Fly  1378 ---IGTWSNDMADDIASFNQEDDDEDDAFH------LPVLPANVKFCDQQTRYGSPTFWDVSNGE 1433

  Fly   389 MN-DGPLCRCSAKSRRIGIRHGIYPGETGYKLCDPNS---------NNAGKLFHYRISISPPTN- 442
            .. .||.   |:::::.|......|.:..:...|.::         :|||.|.:...:.|...:
  Fly  1434 SGFKGPK---SSQNKQGGKGKAKGPAKPTFNYYDSDNSLGSSYDDDDNAGPLNYMHHNYSSDDDD 1495

  Fly   443 -----------FLTK--TPTIIKHDEHEFLFEGFSLLSHVRLSDLPVCKVIRFNIEYTIEYEEEK 494
                       |.:|  ..||....|.|...:..|::.....::....:.....|.:..::|::|
  Fly  1496 VADDIDAGRIAFTSKNEAETIETAQEVEKRQKQLSIIQATNANERQYQQTKNLLIGFNFKHEDQK 1560

  Fly   495 MPENFTIHELDIFFKYLFHELLELVDFNLMPNLPSGNVEESCPAFHFFPRFVRDLPDNGKEVLAM 559
            .|..         .:|                      |||
  Fly  1561 EPAT---------IRY----------------------EES------------------------ 1570

  Fly   560 VEVLRYLLDNSAQLVERQQLLHLNQISQSEWQ----NYVDFIKGMLVTKPGYKPCSLRVDQLDRN 620
            :..|:..:::...||...|.|.|.:...:|.|    :.::.:|.:|   |......|.....||.
  Fly  1571 IAKLKTEIESGGMLVPHDQQLVLKRSDAAEAQVAKVSMMELLKQLL---PYVNEDVLAKKLGDRR 1632

  Fly   621 N---SDLPECVDRETGISHPAIVHFGICHPQLSYAGNPEYQKAWREYVKYRHLMANMSKPSFKDK 682
            .   |||.|.                          |.:: .|..|...|..:....|..::.|.
  Fly  1633 ELLLSDLVEL--------------------------NADW-VARHEQETYNVMGCGDSFDNYNDH 1670

  Fly   683 RK--LEEKEQRLQEMRTQ---GRMKRNITVAISSEGFYRTGIMCDVVQHAMLIPVLTGHLRFHKS 742
            .:  |:||:.:||..|.:   ....:.||.||...||                           |
  Fly  1671 HRLNLDEKQLKLQYERIEIEPPTSTKAITSAILPAGF---------------------------S 1708

  Fly   743 LDLLEESIGYRFKNRYLLQLALTHPSYKENYGTNPDHARNSLTNCGIRQPEYGDRKIHYMNTRKR 807
            .|...:.:|              ||      |.:|.....:||                |:....
  Fly  1709 FDRQPDLVG--------------HP------GPSPSIILQALT----------------MSNAND 1737

  Fly   808 GINTLVSIMSRFGKEHETVSNITHNERLEFLGDAVVEFLSSIHLFFMFPELEEGGLATYRAAIVQ 872
            |||.                     ||||.:||:.:::..:.:|:..:..:.||.|:..|:..|.
  Fly  1738 GINL---------------------ERLETIGDSFLKYAITTYLYITYENVHEGKLSHLRSKQVA 1781

  Fly   873 NQHLALLAKKLQLEEFMLYA--------------------------------------------- 892
            |.:|..|.::.:|.|:|:..
  Fly  1782 NLNLYRLGRRKRLGEYMIATKFEPHDNWLPPCYYVPKELEKALIEAKIPTHHWKLADLLDIKNLS 1846

  Fly   893 -----------------------------HGSDLCHE--------LELRH-----AMANCFEALM 915
                                         ..:|.|::        |..:|     ::|:|.|||:
  Fly  1847 SVQICEMVREKADALGLEQNGGAQNGQLDDSNDSCNDFSCFIPYNLVSQHSIPDKSIADCVEALI 1911

  Fly   916 GALL-------------------------LDGGIKV----------ADEVFTDALFRQDEKLLSI 945
            ||.|                         ||||.:.          |:.|.|         :...
  Fly  1912 GAYLIECGPRGALLFMAWLGVRVLPITRQLDGGNQEQRIPGSTKPNAENVVT---------VYGA 1967

  Fly   946 WKNLPEHPLQEQEPLGDRSCIDSYRVLKELTKFEDSIGIKFKHIRLLARAFTDRSIGFTHLTLGS 1010
            |.. |..||....|..... :|  ::|....:||:|:|.||:....|.:|.|..|.....|| ..
  Fly  1968 WPT-PRSPLLHFAPNATEE-LD--QLLSGFEEFEESLGYKFRDRSYLLQAMTHASYTPNRLT-DC 2027

  Fly  1011 NQRLEFLGDTVLQLICSEYLYRHFPEHHEGHLSLLRSSLVNNRTQAVVCDDLGMPKYAVYANP-- 1073
            .||||||||.||..:.:.:||....:|..|.|:.|||:||||...|.:....|..|:..:.:|
  Fly  2028 YQRLEFLGDAVLDYLITRHLYEDPRQHSPGALTDLRSALVNNTIFASLAVRHGFHKFFRHLSPGL 2092

  Fly  1074 --------------------------------KADLKT-KDRADLLEAFLGALYVDKGLLYCEQF 1105
                                            ..|::. |...|:.|:..||:::|.. :..:..
  Fly  2093 NDVIDRFVRIQQENGHCISEEYYLLSEEECDDAEDVEVPKALGDVFESIAGAIFLDSN-MSLDVV 2156

  Fly  1106 CHV---CLFPRLQLFIMNQDWNDPKSKLQQCC-LTLRTMDGGEPDIPYYKVVEASGPTNTRVYKV 1166
            .||   .:.|.::.|..:.    |||.:::.. |...|...|:|:    |:.:.      |..:|
  Fly  2157 WHVYSNMMSPEIEQFSNSV----PKSPIRELLELEPETAKFGKPE----KLADG------RRVRV 2207

  Fly  1167 AVYFRSKRLATSSGSSIQQAEMNAAKQALENSRDLFPQLDHQKRVIAK 1214
            .|....|......|.:.:.|:..|||.||.       ||..| .:|||
  Fly  2208 TVDVFCKGTFRGIGRNYRIAKCTAAKCALR-------QLKKQ-GLIAK 2247

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
drosha	NP_477436.1	RIBOc	826..942	CDD:197778	37/237 (16%)
		Rnc	973..1196	CDD:440336	67/261 (26%)
Dcr-1	NP_524453.1	DEAD-like_helicase_N	15..208	CDD:475120
		Dicer_PBD	240..344	CDD:277191
		P-loop containing Nucleoside Triphosphate Hydrolases	490..614	CDD:476819
		Dicer_dimer	825..916	CDD:460900
		PAZ_dicer_like	1091..1212	CDD:239209
		RIBOc	1724..1930	CDD:238333	36/242 (15%)
		RIBOc	2008..2172	CDD:238333	42/165 (25%)
		DSRM_DICER	2177..2240	CDD:380680	19/79 (24%)