DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment drosha and DROSHA

DIOPT Version :9

Sequence 1:	NP_477436.1	Gene:	drosha / 35747	FlyBaseID:	FBgn0026722	Length:	1327	Species:	Drosophila melanogaster
Sequence 2:	NP_001369437.1	Gene:	DROSHA / 29102	HGNCID:	17904	Length:	1374	Species:	Homo sapiens

Alignment Length:	1361	Identity:	621/1361 - (45%)
Similarity:	835/1361 - (61%)	Gaps:	200/1361 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 QPPLPPPPVQPA----------------PPPPPPPPEEDLSPPGVGVPSHNYSSNESHSQSSKSL 51
            |.||||.|::|.                ||.|||.|..: :||..|.|           ....:.
Human    90 QGPLPPCPIRPPFPNHQMRHPFPVPPCFPPMPPPMPCPN-NPPVPGAP-----------PGQGTF 142

  Fly    52 DYVYPETPAPYASS---VPSYDPYQQPPAYGYEGYAYNEQAQKYGGQESHYQYQYPA------SG 107
            .::.|....|:...   :|....||.||.|                  ||:.:..|:      :.
Human   143 PFMMPPPSMPHPPPPPVMPQQVNYQYPPGY------------------SHHNFPPPSFNSFQNNP 189

  Fly   108 SSFLYES-------YKYPDRYPAYSSNYRPPSERQ-----------RYTSNSSSQGYHHYP---- 150
            ||||..:       :::...||.    .:.||||:           |:..:|..:|..|..
Human   190 SSFLPSANNSSSPHFRHLPPYPL----PKAPSERRSPERLKHYDDHRHRDHSHGRGERHRSLDRR 250

  Fly   151 --GYSSGR-----RYEQRHDQ-----EHRQIQDSRYAHEPRHGHYAHRQAKGSQHGY---YGSAA 200
              |.|..|     ||...:|:     .||..:.||.....||.|..:|::...:..|   |..:.
Human   251 ERGRSPDRRRQDSRYRSDYDRGRTPSRHRSYERSRERERERHRHRDNRRSPSLERSYKKEYKRSG 315

  Fly   201 RN---------------------QVSDDYSPRGHHERERNETLEKTRAKPKVETERDRLLRQWCS 244
            |:                     :.:|.::|.......|:.:.||.||  :.|.|:||    |..
Human   316 RSYGLSVVPEPAGCTPELPGEIIKNTDSWAPPLEIVNHRSPSREKKRA--RWEEEKDR----WSD 374

  Fly   245 NFC------------EKPEDYVKKMNALSEADAPVESWVRSSPAELYYERTK----SENEVRGRA 293
            |..            ::||:.:...|...|.:.....|:|.:.:|.||....    .::.|.|.:
Human   375 NQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTS 439

  Fly   294 RLQKLCTLFDEELLQRAKRVREKLPVYVPPPRKARRRVCKHKHKSEACSSSSSSDDDSDEDA--- 355
            ||:.|...|:|||..|.::.:...|.:.||..|.          .|...|||.|:.:||||:
Human   440 RLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKL----------DEDLESSSESECESDEDSTCS 494

  Fly   356 -------FKIEQDCCMEELSRKVQHPQRVHADLWHNDAGEMNDGPLCRCSAKSRRIGIRHGIYPG 413
                   |.:     :.|:.||..||.|:|.:||:||.|:|||||||:||||:||.||||.||||
Human   495 SSSDSEVFDV-----IAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPG 554

  Fly   414 ETGYKLCDPNSNNAGKLFHYRISISPPTNFLTKTPTIIKHDEHEFLFEGFSLLSHVRLSDLPVCK 478
            |...|.|.|.:||||:||||||::||||||||..||:|::|:||::|||||:.:|..|:::|:||
Human   555 EEAIKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCK 619

  Fly   479 VIRFNIEYTIEYEEEKMPENFTIHELDIFFKYLFHELLELVDFNLMPNLPSGNVEES---CPAFH 540
            ||||||:|||.:.||.|||||.:..|::|..:||.::|||.|:||...|    .|:|   ||.||
Human   620 VIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPL----FEDSPPCCPRFH 680

  Fly   541 FFPRFVRDLPDNGKEVLAMVEVLRYLLDNSAQLVERQQLLHLNQISQSEWQNYVDFIKGMLVTKP 605
            |.|||||.|||.|||||:|.::|.|||..|..||..:::.::.|..:.|||.|.:..|||:||.|
Human   681 FMPRFVRFLPDGGKEVLSMHQILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNP 745

  Fly   606 GYKPCSLRVDQLDRN--NSDLPECVDRETGISHPAIVHFGICHPQLSYAGNPEYQKAWREYVKYR 668
            |.||.|:|:|||||.  |.|:         |:.|.||||||...||||||:|:|||.|:.|||.|
Human   746 GTKPSSVRIDQLDREQFNPDV---------ITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLR 801

  Fly   669 HLMANMSKPSFKDKRKLEEKEQRLQEMRTQGRMKRNITVAISSEGFYRTGIMCDVVQHAMLIPVL 733
            ||:||..|....||:||.::|:.||::|.:..|:|.:||.:||:||::|||..||.||||::|||
Human   802 HLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVL 866

  Fly   734 TGHLRFHKSLDLLEESIGYRFKNRYLLQLALTHPSYKENYGTNPDHARNSLTNCGIRQPEYGDRK 798
            |.|:|:|:.|..|::.|||.|::|.|||||:||||:..|:|.|||||||||:|||||||:|||||
Human   867 THHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRK 931

  Fly   799 IHYMNTRKRGINTLVSIMSRFGKEHETVSNITHNERLEFLGDAVVEFLSSIHLFFMFPELEEGGL 863
            :|:|:.||:|||||::||||.|::..|.|.|.||||||||||||||||:|:||:::||.||||||
Human   932 VHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGL 996

  Fly   864 ATYRAAIVQNQHLALLAKKLQLEEFMLYAHGSDLCHELELRHAMANCFEALMGALLLDGGIKVAD 928
            ||||.|||||||||:|||||:|:.|||||||.|||.|.:||||||||||||:||:.|:|.::.|.
Human   997 ATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGSLEEAK 1061

  Fly   929 EVFTDALFRQDEKLLSIWKNLPEHPLQEQEPLGDRSCIDSYRVLKELTKFEDSIGIKFKHIRLLA 993
            ::|...|| .|..|..:|.|.|.||||.|||..||..|::..||::||:||::||:.|.|:||||
Human  1062 QLFGRLLF-NDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHVRLLA 1125

  Fly   994 RAFTDRSIGFTHLTLGSNQRLEFLGDTVLQLICSEYLYRHFPEHHEGHLSLLRSSLVNNRTQAVV 1058
            ||||.|::||.|||||.|||:|||||:::||:.:|||:.|||:||||||:|||||||||||||.|
Human  1126 RAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKV 1190

  Fly  1059 CDDLGMPKYAVYANPKAD----LKTKDRADLLEAFLGALYVDKGLLYCEQFCHVCLFPRLQLFIM 1119
            .::|||.:||: .|.|..    |:||..|||||:|:.|||:||.|.|...|.:||.||||:.||:
Human  1191 AEELGMQEYAI-TNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFIL 1254

  Fly  1120 NQDWNDPKSKLQQCCLTLRTMDGGEPDIPYYKVVEASGPTNTRVYKVAVYFRSKRLATSSGSSIQ 1184
            |||||||||:|||||||||| :|.|||||.||.::..||::.|.|.|||||:.:|:....|.|||
Human  1255 NQDWNDPKSQLQQCCLTLRT-EGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQ 1318

  Fly  1185 QAEMNAAKQALENSRDLFPQLDHQKRVIAKSIKKQTGNELDNDSDRQHQE------EKIKR 1239
            ||||.||..|||...  |||:.||||.|.:..:::.   .:...:|:|||      |.||:
Human  1319 QAEMGAAMDALEKYN--FPQMAHQKRFIERKYRQEL---KEMRWEREHQEREPDETEDIKK 1374

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
drosha	NP_477436.1	DLL_N	64..136	CDD:289198	19/98 (19%)
		Rnc	738..>974	CDD:223644	152/235 (65%)
		RIBOc	826..942	CDD:197778	80/115 (70%)
		rnc	973..1196	CDD:234633	140/226 (62%)
		RIBOc	990..1117	CDD:197778	82/130 (63%)
		DSRM	1125..1196	CDD:238007	43/70 (61%)
DROSHA	NP_001369437.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..95		3/4 (75%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	130..406		63/314 (20%)
		U2AF_lg	217..>317	CDD:273727	23/99 (23%)
		Necessary for interaction with DGCR8 and pri-miRNA processing activity. /evidence=ECO:0000269\|PubMed:26027739, ECO:0000269\|PubMed:26748718	390..1365		546/1010 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	452..497		15/54 (28%)
		RIBOc	959..1079	CDD:238333	81/120 (68%)
		rnc	1105..1333	CDD:234633	142/229 (62%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165144444
Domainoid	1	1.000	615	1.000	Domainoid score	I182
eggNOG	1	0.900	-	-		E1_COG0571
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H8293
Inparanoid	1	1.050	1104	1.000	Inparanoid score	I231
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48888
OrthoDB	1	1.010	-	-		D58671at33208
OrthoFinder	1	1.000	-	-		FOG0003184
OrthoInspector	1	1.000	-	-		oto88467
orthoMCL	1	0.900	-	-		OOG6_101368
Panther	1	1.100	-	-	LDO	PTHR11207
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R885
SonicParanoid	1	1.000	-	-		X4740
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.800