DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment didum and MYO4

DIOPT Version :9

Sequence 1:	NP_724569.1	Gene:	didum / 35680	FlyBaseID:	FBgn0261397	Length:	1800	Species:	Drosophila melanogaster
Sequence 2:	NP_009373.1	Gene:	MYO4 / 851204	SGDID:	S000000027	Length:	1471	Species:	Saccharomyces cerevisiae

Alignment Length:	1622	Identity:	520/1622 - (32%)
Similarity:	803/1622 - (49%)	Gaps:	271/1622 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 MLYAQGAKIWVPHADLVWESATL-EESYRKGAGFLKICTDSGKLKEVKLKA-----DGSDLPPLR 64
            |.:..|.|.|.||.:..|....: :..:.:|...|::..:.|:...::..:     |...||.||
Yeast     1 MSFEVGTKCWYPHKEQGWIGGEVTKNDFFEGTFHLELKLEDGETVSIETNSFENDDDHPTLPVLR 65

  Fly    65 NPAILVGQNDLTTLSYLHEPGVLHNLRVRFCERQIIYTYCGIILVAINPYAEMP-LYGPSIIRAY 128
            ||.||...:||||||||:||.|||.::.|:...| ||||.||:|:|.||:.::. ||...:|:.|
Yeast    66 NPPILESTDDLTTLSYLNEPAVLHAIKKRYMNGQ-IYTYSGIVLIAANPFDKVDHLYSREMIQNY 129

  Fly   129 RGHAMGDLEPHIFALAEEAYTKLERENCNLSIIVSGESGAGKTVSAKYAMRYFAAVGGSESE--- 190
            ......:||||:||:|||||..:..|..|.:::|||||||||||||||.|||||:|..|.:.
Yeast   130 SSKRKDELEPHLFAIAEEAYRFMVHEKANQTVVVSGESGAGKTVSAKYIMRYFASVQESNNREGE 194

  Fly   191 ---TQVERKVLASSPIMEAFGNAKTTRNDNSSRFGKFTKLLFRNQMGVMFLQGATMHTYLLEKSR 252
               :|:|.::||::||||||||||||||||||||||:.::||.....:   :|:.:.||||||||
Yeast   195 VEMSQIESQILATNPIMEAFGNAKTTRNDNSSRFGKYLQILFDENTTI---RGSKIRTYLLEKSR 256

  Fly   253 VVYQAQGERNYHIFYQLCAARSK--YPELVLDHQDKFQFLNMGGAPEIERVSDAEQFNETVQAMT 315
            :|||.:.|||||||||:.....:  ..||.|.....:.:.|.||.|.|..:.:|.::..|..|::
Yeast   257 LVYQPETERNYHIFYQILEGLPEPVKQELHLSSPKDYHYTNQGGQPNIAGIDEAREYKITTDALS 321

  Fly   316 VLGFSIQQIADIVKILAGILHLGNIQVSKKFNE---GSEEEDSDSCDIFHNDIHLQITADLLRVS 377
            ::|.:.:....|.|||||:||:|||::....|:   .|||:            :|||..:||.:.
Yeast   322 LVGINHETQLGIFKILAGLLHIGNIEMKMTRNDASLSSEEQ------------NLQIACELLGID 374

  Fly   378 ADDLRRWLLMRKIESVNEYVLIPNSIEAAQAARDALAKHIYAKLFQYIVGVLNKS-----LNNGS 437
            ..:..:|::.::|.:.:|.::...:...|..|||::||.||:.||.::|..:||:     |:...
Yeast   375 PFNFAKWIVKKQIVTRSEKIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQD 439

  Fly   438 KQCSFIGVLDIYGFETFEVNSFEQFCINYANEKLQQQFNQHVFKLEQEEYLKEGITWTMIDFYDN 502
            ...||||:|||||||.||.|||||||||||||||||:|||||||||||||:||.|.|:.|:|.||
Yeast   440 HVFSFIGILDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDN 504

  Fly   503 QPCIDLIESRLGVLDLLDEECRMPKGSDESWAGKLIGKCNKFPH---FEKPRFGTTSFFIKHFSD 564
            ||||||||::||:|.|||||.|:|.|||||||.||....||.|.   |.|||||.|.|.:.|::.
Yeast   505 QPCIDLIENKLGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTKFIVSHYAV 569

  Fly   565 TVEYDVNGFLEKNRDTVSKELTQVLSESNMSLAKQVMTLEEIDTLCVDSAKSSTLGGRVVISAGR 629
            .|||:|.||:|||||:||.....|...:...:.||::...|:.:   |.|
Yeast   570 DVEYEVEGFIEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRS---DDA--------------- 616

  Fly   630 KQQGNDTRRRVVPSK--QHRKTVGSQFQESLASLISTLHATTPHYVRCIKPNDDKVAFKWETAKI 692
            .::.|..::.::|::  |.:.|:||.|::||..|::.:::|..||:||||||.:|..::::...:
Yeast   617 PEEQNTEKKIMIPARLSQKKPTLGSMFKKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMV 681

  Fly   693 IQQLRACGVLETVRISAAGFPSRWLYPDFYMRYQLL---------VYRSKLDKNDMKLSCRNIVM 748
            :.||||||||||:|||.|||||||.:.:|..||.||         :|...|.|..:...|::|:.
Yeast   682 LSQLRACGVLETIRISCAGFPSRWTFDEFVQRYFLLTDYSLWSGILYNPDLPKEAIVNFCQSILD 746

  Fly   749 KWIQDEDKYRFGNTQIFFRAGQVAFLEQVRANLRKKYITIVQSVVRRFVYRRQFLRIQKVINGIQ 813
            ..|.|..||:.|||:|||:||.:||||::|.|...:...|:|..:|...||.|:|:..:.|...|
Yeast   747 ATISDSAKYQIGNTKIFFKAGMLAFLEKLRTNKMNEICIIIQKKIRARYYRLQYLQTMESIKKCQ 811

  Fly   814 KHARGYLARERTQKMREARAGLILSKYARGWLCRRRYLRLR-HSISGIQTYARGML----ARNKF 873
            ...|..|.|.|.....:.||.::|....|. |.:|.|.|.. ..|..:|...:..|    ...||
Yeast   812 SQIRSLLVRTRVDHELKTRAAILLQTNIRA-LWKREYYRAAIGQIIKLQCTCKRKLILDSVNRKF 875

  Fly   874 HAMRDHYRAVQIQRFVRGALARRAYQKRRRNIIICQAAIRRFLARRKFKRMKAEAKTISHMENKY 938
            ..|    .||.||.::|....:..|:..:|:.|:.|:|:|..||||::..::.|.:..:...:..
Yeast   876 MLM----AAVIIQSYIRSYGHKTDYRTLKRSSILVQSAMRMQLARRRYIVLQKEVEERNIRASYG 936

  Fly   939 MGL--------ENKIISMQQRIDELNR-------DNSNLKHKTSE-----ISVLKMKLELKKTLE 983
            :||        .:.|::::...|...|       |.||:..|..:     ::....|:...|||:
Yeast   937 IGLLEEAIEFKNSFILNLEMLNDSYTRLTQLLQGDLSNIPSKQRQEYETIVNGYNDKISKLKTLQ 1001

  Fly   984 AEFKNVKAACQDKDKLIEALNKQLEAERDEKMQLLEENGHAQEEWISQKQTWRQENEELRRQIDE 1048
            .|..|   ....|:.|.|...||....:.....|....|:.......:.::.:||...:...|.:
Yeast  1002 VEIMN---TLNKKNALKERKKKQSSLIQSHMQSLAAIKGNKPSRLSDEVKSMKQELAFIENVIAQ 1063

  Fly  1049 IIDMAKNAEVNQRNQEDRMLAEIDNRELNEAYQRAIKDK--EVIENE--NFMLKEELSRLTAGSF 1109
            ......:|     |:.|::      :.|..|.|: :|.|  .||..|  |..|.|.|..|..  :
Yeast  1064 DFTTTYSA-----NKNDKV------KGLGIAGQQ-VKPKLVNVIRRESGNPDLLELLMDLNC--Y 1114

  Fly  1110 SLHARKASNASSQNEDDVGYASAKNTLDINRPPDLLSKNYSYNDSTSLVVKLRSILEEEKQKHKV 1174
            :|...:            ||....|..::| ..::|...:......|.:|:...:::..|...||
Yeast  1115 TLEVTE------------GYLKKVNVTEVN-GDNVLGPIHVITTVVSSLVRNGLLIQSSKFISKV 1166

  Fly  1175 LQEQYIKLSSRHKPTEDSFRVSELEVENEKLRSEYDQLRTSIKHGV----EINELNAQHAALQEE 1235
            |                      |.||:..:....|:   ::..|:    .::.|.| .||.|:.
Yeast  1167 L----------------------LTVESIVMSLPKDE---TMLGGIFWLSNLSRLPA-FAANQKT 1205

  Fly  1236 VRRRR--EECIQLKAVLLQQSQSMRSLEPESLQMRGNDV-NELMEAFHSQKLINRQLESELKAIT 1297
            :....  :|..:|..:.|                  ||: ||.::.|  .|:.:..|...:|
Yeast  1206 LYEANGGDEKDKLTLIYL------------------NDLENETLKVF--DKIYSTWLVKFMK--- 1247

  Fly  1298 EEHNSKLVEMTQEI--ERL-NNEKDELQKVMFESIDEFEDSNVDTLRQNDRYLRRELQKAVAQFL 1359
              |.|..:|:...:  |:| .|..||....:|..::||:                   ..:.:|.
Yeast  1248 --HASAHIEIFDMVLNEKLFKNSGDEKFAKLFTFLNEFD-------------------AVLCKFQ 1291

  Fly  1360 LVQE-ELKLANAKLKAYRQDGGQLEHKIEEEMIRNKSNGTSADVGANVTKQKSQNPQGLMKFHSS 1423
            :|.. ..|:.|..||       .|...:..::|               ||..:.|.:     :..
Yeast  1292 VVDSMHTKIFNDTLK-------YLNVMLFNDLI---------------TKCPALNWK-----YGY 1329

  Fly  1424 DLDKILQRLLSALTPRTVVGLLPGFPAYLIFMCIRYTDLTNADD--------------DVRELLS 1474
            ::|:.::||:|...|| :..:.|.....:..:.|....::|.::              .::.:|.
Yeast  1330 EVDRNIERLVSWFEPR-IEDVRPNLIQIIQAVKILQLKISNLNEFKLLFDFWYALNPAQIQAILL 1393

  Fly  1475 KFVIQIKKMHRTPHPIENRVIWLVNSITLLN----------LMKQYGDVDEYVKFNTEKQNQ 1526
            |:....|.....|:.|.|   :|.|.|...|          |..|:.....:::::|....|
Yeast  1394 KYKPANKGEAGVPNEILN---YLANVIKRENLSLPGKMEIMLSAQFDSAKNHLRYDTSAITQ 1452

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
didum	NP_724569.1	MYSc	65..778	CDD:214580	341/743 (46%)
		MYSc_Myo5	84..767	CDD:276831	321/713 (45%)
		IQ	900..922	CDD:197470	9/21 (43%)
		GBP_C	<971..1086	CDD:303769	22/114 (19%)
		coiled coil	1049..1068	CDD:293879	3/18 (17%)
		coiled coil	1077..1086	CDD:293879	2/8 (25%)
		JAKMIP_CC3	<1190..1299	CDD:292653	21/115 (18%)
		Myo5_CBD	1424..1792	CDD:271254	23/127 (18%)
MYO4	NP_009373.1	COG5022	2..1471	CDD:227355	519/1621 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	611	1.000	Domainoid score	I20
eggNOG	1	0.900	-	-		E2759_KOG0160
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	767	1.000	Inparanoid score	I68
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000014
OrthoInspector	1	1.000	-	-		otm46742
orthoMCL	1	0.900	-	-		OOG6_100101
Panther	1	1.100	-	-	O	PTHR13140
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X244
TreeFam	1	0.960	-	-
	11	10.820