DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dscam1 and robo4

DIOPT Version :9

Sequence 1:	NP_001246162.1	Gene:	Dscam1 / 35652	FlyBaseID:	FBgn0033159	Length:	2038	Species:	Drosophila melanogaster
Sequence 2:	XP_689255.3	Gene:	robo4 / 560765	ZFINID:	ZDB-GENE-020809-1	Length:	1134	Species:	Danio rerio

Alignment Length:	1154	Identity:	231/1154 - (20%)
Similarity:	386/1154 - (33%)	Gaps:	381/1154 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   601 VAKNQEG-------------------YSARGSLEVQVMVLPQIVPFAYEDLINMGDSIDLFCQIQ 646
            ||:..||                   :..:||..|..:.||:||....:.::.:|....|.|:.:
Zfish    31 VAQRSEGKGHLRHRLMHQRASHRDRAHRRKGSRLVSEINLPRIVHHPSDVVVRVGSPATLSCRAE 95

  Fly   647 KGDRPIKVHWSFERSAGDYGFDQVQPQMRTNRISEKTSMISIPSAS-------PAHTGRD----- 699
            ....| .:.|          ....|| :.|:::..::..|.:|..|       |...|:.
Zfish    96 GNPEP-TIQW----------LRNGQP-LDTDKMDAQSQPIVLPDGSLFFFSVVPGRKGQSHEAVY 148

  Fly   700 TCIASNKAGTTTYSVDLTVNVP---PRWILEPTDKAFAQGSDAKVECKAD-GFPKPQVTWKK--- 757
            .|||.|..|..| |.:.::::.   ..:.::|:|...|.|..|.:.|... |.|:|.|||:|
Zfish   149 ACIAHNSIGNAT-SRNASLHIAALREDFRVQPSDVEVAIGEMATINCSPPVGHPEPNVTWRKDGI 212

  Fly   758 AVGDTPGEYKDLKKSDNIRVEEGTLHVDNIQKTNEGYYLCEAINGIGSGLSAVIMISVQAPPEFT 822
            .:..:...|.:||         |.|.:...||.:.|.|.|.|.|.||...|....:||.|.|...
Zfish   213 LINSSNEHYTELK---------GKLIIAPAQKNDSGVYSCIASNMIGVRESRAARLSVLAKPVLL 268

  Fly   823 EKLRNQTARRGEPAVLQCEAKGEKPIGILWNMNNMRLDPKNDNRYTIREEILSTGVMSSLSIKRT 887
            .|..:.:.:.||.|...|||.|:....|.|:...   .|..:.||.|..:       .||.|...
Zfish   269 RKPEDVSVQLGESAQFFCEADGDPMPSIEWSREQ---GPLPNGRYLINPD-------HSLQIHYV 323

  Fly   888 ERSDSALFTCVATNAFGSDDASINMIVQ------------EVPEMPYALK---VLDKSGRSVQLS 937
            ...|...::|...|..|...||..::|:            |:..:..:|:   |:..:....|:.
Zfish   324 TAQDMGRYSCTVENKLGVSVASAQLLVEDAGGTRLRDLHKELSALRVSLENVTVMSTASNMSQVM 388

  Fly   938 WAQPYDGNSP--LDRYIIEFKR---SRASWSEIDRVIVPGHTTEAQVQKLSPATTYNIRIVAENA 997
            |.....|:.|  |:.:.:.::.   :.:.|: ..||..|...|.....|......:.:|....:.
Zfish   389 WKLQSFGSQPHYLEGFEVLYRSLLPASSDWT-AQRVPQPSLHTHVGPLKRGYKYEFKVRPYGSSL 452

  Fly   998 IGTSQSSEAVTIITAEEAPSGKPQNIKVE-PVNQT-TMRVTWKPPPRTEWNGEILGYYVGYKLSN 1060
            .|...::..:.:  .|..||..||::.:. |..:. |:.::|:|||....||.|.||.|....|.
Zfish   453 YGRESNTRHLRV--PEIVPSAAPQDVSITMPAERNDTVHLSWEPPPHDAHNGIIQGYQVWCVESE 515

  Fly  1061 TNSSYVFETINFITEEGKEHNLELQNLRVYTQYSVVIQAFNKIGAGPLSEEEK---QFTAEGTPS 1122
            ..:|:     |:..:.| .|::|:..|.....|.|.:.|.|..|.|..|:..|   :...|.||.
Zfish   516 EQTSF-----NWTVDSG-THSIEIFTLLPSRLYWVTVAAVNGAGVGVQSDPYKLLIESRLEATPY 574

  Fly  1123 QPP--SDTACTTLTSQTIRVG------W--------------VSPP-----------LESANGVI 1154
            |..  |.:...::..:.:.:|      |              :.|.           |||.:.:|
Zfish   575 QTGILSMSHFVSVIKEPVFIGSVGTLLWCVLMITAVFLYRRHIRPANPSGKSSGLYRLESEDLII 639

  Fly  1155 KTYKVVYAPSDEWY---------DETKRHYKKTASSDTVLHGLKKYT------------NYTMQV 1198
            | :::. ||...|.         .|.|:.....:..::   ||::.|            ...:.:
Zfish   640 K-HRMA-APDSPWISGGWRPGSNSEPKQGLWTPSQENS---GLRRTTLPITVKKDPNPLRSAVPI 699

  Fly  1199 LATTAG----------GDGVRSV--PIHCQTEPDVPEAPTD-------------VKALVMGNAAI 1238
            :....|          |:|:::.  |..|   |.:|...:.             ||..|:.....
Zfish   700 VPDNCGVYGTFYVDLTGNGLKTFNSPGRC---PRMPHGNSQLQNSETIRITEPIVKTAVVRELQA 761

  Fly  1239 LVSWRP--PAQPN-GIITQYTVYSKAEGAETETKTQ----------------------------- 1271
            | .|:.  ||||| |:        |.|..|...|.:
Zfish   762 L-PWKRTLPAQPNMGV--------KKESWEKNVKRELHAVKSAPLMPLNQQALAVCSAPNHQQRF 817

  Fly  1272 ------------------KVPHYQMSFEATELEKNKP-----------YEFWVTAST--TIGEGQ 1305
                              ::.||..|.:..::..:.|           .|...|.||  |:..|.
Zfish   818 SQHPAGGVSEPVKPLSSPRILHYSASLQLVDMLPSPPPLPMEDNHSLSSEDESTRSTKLTVDIGS 882

  Fly  1306 QSKSIVA------------MPSDQVPAKIA-SFDDTFTATFKEDAKMPCLAVGAPQP-------- 1349
            |.....|            .|::..||::: |:....||:|       ||:....|.
Zfish   883 QQSVCAASGLQGPASANTGCPNNHSPAQLSPSYSHLSTASF-------CLSTDDYQDTTLSTQGH 940

  Fly  1350 ----EITWKIKGVEFS----------------------ANDR-----------------MRVLP- 1370
                ||:.|.:|...|                      ..||                 :|..|
Zfish   941 TQYMEISPKPQGQSTSHQSTPSMPRPFSPTPTLGYICGPGDREMDEEQETVPIGLRRATLRSTPS 1005

  Fly  1371 ------DGSL------------------LIKSVNRQDAGDYSCHAENSIAKDSITHKLIVLAPPQ 1411
                  :|||                  :|.|.:.....|.:.....::..:|::..|...:||.
Zfish  1006 SCCSEWEGSLWNGWGSVSESNVTSARTSIISSSDCSFINDANFARVLALTAESMSGNLSDFSPPA 1070

  Fly  1412 SPHVTLSATTTDALTVKLKPHE--GDTAPLHGYTLHYKPEFGEWETSEV 1458
            ||           |:|...|.|  |:..||..:         :|.|:.|
Zfish  1071 SP-----------LSVLFPPRECFGEVEPLPVW---------DWSTAWV 1099

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dscam1	NP_001246162.1	Ig	38..130	CDD:299845
		IG	57..133	CDD:214652
		IG_like	267..343	CDD:214653
		Ig	269..340	CDD:143165
		IG_like	353..425	CDD:214653
		IGc2	361..413	CDD:197706
		I-set	433..527	CDD:254352
		IGc2	446..517	CDD:197706
		I-set	533..618	CDD:254352	7/35 (20%)
		IGc2	544..607	CDD:197706	2/5 (40%)
		Ig	641..714	CDD:143165	18/84 (21%)
		IGc2	735..804	CDD:197706	22/72 (31%)
		I-set	819..914	CDD:254352	24/94 (26%)
		Ig	833..921	CDD:299845	24/99 (24%)
		FN3	918..1011	CDD:238020	15/100 (15%)
		FN3	1018..1116	CDD:238020	29/102 (28%)
		FN3	1124..1217	CDD:238020	22/158 (14%)
		FN3	1222..1312	CDD:238020	27/165 (16%)
		Ig	1339..1406	CDD:299845	20/142 (14%)
		FN3	1409..1499	CDD:238020	14/52 (27%)
		FN3	1504..1584	CDD:238020
		Dscam_C	1890..2008	CDD:289151
robo4	XP_689255.3	Ig1_Robo	70..169	CDD:143317	24/111 (22%)
		I-set	71..168	CDD:254352	23/109 (21%)
		I-set	175..261	CDD:254352	28/94 (30%)
		Ig2_Robo	177..261	CDD:143201	28/92 (30%)
		I-set	265..350	CDD:254352	24/94 (26%)
		Ig	282..350	CDD:299845	20/77 (26%)
		FN3	373..448	CDD:214495	13/75 (17%)
		FN3	472..560	CDD:238020	28/93 (30%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3510
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10075
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.910