DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dscam1 and ROBO4

DIOPT Version :9

Sequence 1:	NP_001246162.1	Gene:	Dscam1 / 35652	FlyBaseID:	FBgn0033159	Length:	2038	Species:	Drosophila melanogaster
Sequence 2:	NP_061928.4	Gene:	ROBO4 / 54538	HGNCID:	17985	Length:	1007	Species:	Homo sapiens

Alignment Length:	1095	Identity:	213/1095 - (19%)
Similarity:	357/1095 - (32%)	Gaps:	398/1095 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly   721 PPRWILEPTDKAFAQGSDAKVECKADGFPKPQVTW---KKAVGDTPGEYKDLKKSDNIRVEEGTL 782
            ||:.::.|.|:.|.....|::.|:|.|.|.|.:.|   .:.:...|.:...|       :.:|||
Human    31 PPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHL-------LPDGTL 88

  Fly   783 -----------HVDNIQKTNEGYYLCEAINGIGSGLSAVIMISVQAPPE-FTEKLRNQTARRGEP 835
                       |......|:.|.|.|||.|.:|:.:|....:||....| |..:.|:..|..||.
Human    89 LLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQ 153

  Fly   836 AVLQCEAK-GEKPIGILW--NMNNMRLDPKNDNRYTIREEILSTGVMSSLSIKRTERSDSALFTC 897
            ..|:|... |.....:.|  :...:.|.|   .|:|:     |.|   ||.:.|.|:||...:.|
Human   154 FTLECGPPWGHPEPTVSWWKDGKPLALQP---GRHTV-----SGG---SLLMARAEKSDEGTYMC 207

  Fly   898 VATNAFG-SDDASINMIVQEVPEMPYALKVL-------------------DKSGRSVQLSW--AQ 940
            ||||:.| .:..:..:.:||..:....:::|                   .|...:|.|||  :.
Human   208 VATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSG 272

  Fly   941 PYDGNSPLDRYIIEFKRSR------ASWSEIDRVIVPGHTTEAQVQKLSPATTYNIRIVAENAIG 999
            |   .:|...|...|:...      |.|:|   .::.|..: |::..|.....|..::...:...
Human   273 P---AAPAQSYTALFRTQTAPGGQGAPWAE---ELLAGWQS-AELGGLHWGQDYEFKVRPSSGRA 330

  Fly  1000 TSQSSEAVTIITAEEAPSGKPQNIKVEPVNQTTMRVTWKPPPRTEWNGEILGYYVGYKLSNTN-- 1062
            ....|..:.:...|:.||..||.:.::|.|.|.. |:|.|||....||.|.||.| :.|.||:
Human   331 RGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVF-VSWVPPPAENHNGIIRGYQV-WSLGNTSLP 393

  Fly  1063 --------------------SSY----------------------VFETINFITEEGKEHN-LEL 1084
                                .||                      :.:.:...|:|..||. ..|
Human   394 PANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTL 458

  Fly  1085 QNLRV------------------------------------------YTQYSVVIQ--------- 1098
            :.||.                                          ||....:::
Human   459 EQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQ 523

  Fly  1099 --------------------AFNKIGAG---------------------PLSEEEKQF----TAE 1118
                                ..:::||.                     ||..:...|    .||
Human   524 WLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAE 588

  Fly  1119 ---GTPSQP-PSDTACTTLTSQTIRVGWVSPPLESANGV-----IKTYKVVYAPSDEWYDETKRH 1174
               .||::| |...|...|..|   :..:|.|..|::.:     :.:.::..||::.|..:.|:.
Human   589 LPSSTPARPSPQVPAVRRLPPQ---LAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQE 650

  Fly  1175 YKKTASSDTVLHGLKKYTNYTMQVLATTAGGDGVRSVPIHCQTEPDVPEAPTDVKALVMGNAAIL 1239
            .:. |:|..:|.|     ::::::.|...|..|.:::   .|:...||:|              |
Human   651 LQH-ANSSPLLRG-----SHSLELRACELGNRGSKNL---SQSPGAVPQA--------------L 692

  Fly  1240 VSWR---PP--AQPNGIITQYTVYSKAEGAET-ETKTQKV-----------------------PH 1275
            |:||   |.  :..|.::|::...:.....|| .|::|:.                       |.
Human   693 VAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPC 757

  Fly  1276 YQMSFEATELEKNKPYEFWVTAS--TTIGEGQQS---------------------KSIVAMPSDQ 1317
            ...|.:|:.|....|....:::|  :::||.|.|                     .|:..||...
Human   758 SPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAP 822

  Fly  1318 VPAKIASFDDTFTATFKEDAKMPCLAVG--------APQPEIT-----------WKIKGVEFSAN 1363
            .|.....:....||:...|.......||        .|:|.:|           |.....:.:|:
Human   823 SPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAAS 887

  Fly  1364 DRMRVL--PDGSLLIKSVNRQDAGDYSCHAENSIAKDSITHKL---------IVLAPPQSPHVTL 1417
            .|..::  .|||.|         .|.......::|.||....|         |..:.|.||...:
Human   888 ARASLVSSSDGSFL---------ADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEI 943

  Fly  1418 SATTTDALTVKLKPHEGDTAPLHGYTLHYKPEFGEW-ETSEVSVDSQKHNIEGLLCGSRYQVYAT 1481
            ..|...:|            ||    ..::|   :| |..|||     |.
Human   944 FLTPNLSL------------PL----WEWRP---DWLEDMEVS-----HT--------------- 969

  Fly  1482 GFNNIGAGEASDILNTRTKGQKPKLPEKPRFIEVSSNSVSLHFKAWKDGGCPMSH 1536
              ..:|.|                :|..|...::||....||.:..|.|..|:.:
Human   970 --QRLGRG----------------MPPWPPDSQISSQRSQLHCRMPKAGASPVDY 1006

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dscam1	NP_001246162.1	Ig	38..130	CDD:299845
		IG	57..133	CDD:214652
		IG_like	267..343	CDD:214653
		Ig	269..340	CDD:143165
		IG_like	353..425	CDD:214653
		IGc2	361..413	CDD:197706
		I-set	433..527	CDD:254352
		IGc2	446..517	CDD:197706
		I-set	533..618	CDD:254352
		IGc2	544..607	CDD:197706
		Ig	641..714	CDD:143165
		IGc2	735..804	CDD:197706	20/82 (24%)
		I-set	819..914	CDD:254352	28/99 (28%)
		Ig	833..921	CDD:299845	26/91 (29%)
		FN3	918..1011	CDD:238020	18/119 (15%)
		FN3	1018..1116	CDD:238020	35/238 (15%)
		FN3	1124..1217	CDD:238020	19/98 (19%)
		FN3	1222..1312	CDD:238020	24/141 (17%)
		Ig	1339..1406	CDD:299845	17/96 (18%)
		FN3	1409..1499	CDD:238020	16/90 (18%)
		FN3	1504..1584	CDD:238020	9/33 (27%)
		Dscam_C	1890..2008	CDD:289151
ROBO4	NP_061928.4	Ig	31..133	CDD:299845	28/108 (26%)
		I-set	32..132	CDD:254352	26/106 (25%)
		I-set	139..220	CDD:254352	27/91 (30%)
		Ig2_Robo	141..225	CDD:143201	26/94 (28%)
		fn3	350..432	CDD:278470	21/83 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	531..551		1/19 (5%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	713..793		14/79 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	806..826		4/19 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	971..1007		11/52 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910