Protein Alignment Epac and rapgef2a

DIOPT Version :9

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: XP_005170987.2
• Gene: rapgef2a / 793952
• ZFINID: ZDB-GENE-090313-280
• Length: 1486
• Species: Danio rerio
• Alignment Length: 1202
• Identity: 267/1202 - (22%)
• Similarity: 455/1202 - (37%)
• Gaps: 374/1202 - (31%)

  Fly     8 AMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGRFWYA 72
            |:.|.|.||..:|::::...|..:|.|..|....|:.:.....||..:....|:...:.|..||.
Zfish    17 AIMKIPADRTQQDLQIVYSYLHGMEALSNLREHQLRLMCETVRYEQHDANEVLYYPDDIGTCWYI 81

  Fly    73 VLGGSLEVRYHAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRVEQQD--- 134
            :|.||:.:          |.::.|..    ::||:.....|.|....:.....|::.|:..|
Zfish    82 LLSGSVFI----------KESMFLPR----SSFGKRSAGSLRRGCECIVLEASEMIVVDYMDDND 132

  Fly   135 ---------------FRLIWEK-NKELMNDIFTNCKLKNGFGPSVQATAAATSPTKRPLSPDHPN 183
                           ||.|.:: .::.:.|...|..:.....|....:....|......|..||:
Zfish   133 EYFQRQASHRQSRRRFRKINQRGERQTIIDTVDNYPVSKPPLPPGYHSECPKSQLPTDFSKLHPS 197

  Fly   184 PALPITETPSPAMNRMGWALRTLLVADN-SSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPIVHT 247
            .:.....|.|.:        |:.:.:|: ||.|.|
Zfish   198 DSCHSHMTSSQS--------RSSIASDSGSSSLSD------------------------------ 224

  Fly   248 RAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQA----------EDLG 302
                                           :|:....|.|....:|:|:.          ||:|
Zfish   225 -------------------------------IYQATESECGDMDLSGLPETAVDSDEDDDDEDVG 258

  Fly   303 AANEHIREALSALFQRGPDATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSI 367
            ...:|       |..|.   .:|..|.|...:||.:::|.:.|.:..:.|.:.||.|::|||.::
Zfish   259 RGTDH-------LMSRD---IVRDCLEKDPMDRTDDDIEQLLEFVQQLPAFASLSVSVRRELCAV 313

  Fly   368 FVFEAHAQAGTILFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATI 432
            .||....:||||:.|.|:|..||.::|.|:|: ||:..|...::..|..||....:........:
Zfish   314 MVFAVVERAGTIVLNHGEELDSWSVILNGAVE-VIYLDGRSESVCMGGSFGVCPSMEKQLMIGVM 377

  Fly   433 VLKENNCHLLRVDKEHFNRILRDVEANTLRLQEHGKDVLV-----LERVAKQRGQHSAFKYTVMS 492
            ..|.::|..:.:.::.:..||..||.||.|::|.|:.|:|     |:|...::|      :.|:.
Zfish   378 RTKVDDCQFVCIAQQDYCCILNQVEQNTQRVEEEGEIVMVKEHRELDRTGTRKG------HIVVK 436

  Fly   493 GTPAKMLEHLLETRLGQSVGGMDP-FLDDFLLTHIVFMPVVQLVDE-LANYFHCDAHEDAQTPED 555
            ||..::::||:|   ..|:  :|| :::|||||:..|:|...::.: |.::|:.....|
Zfish   437 GTAERLMQHLVE---DHSI--VDPTYIEDFLLTYRTFLPSPLILGQRLLDWFNDPCLRD------ 490

  Fly   556 REYIINFKKRVIQFMQKWVMAVRH-AAFE-EPSVCDFIED------------------LAAEVEA 600
                     ||.:.:..||.  .| :.|| :|.:..|:|:                  :|...:|
Zfish   491 ---------RVTRVVLLWVN--NHFSDFEGDPDMTSFLEEFQSNLEREKMTGQLRLLNIACAAKA 544

  Fly   601 DP-------------------------------------------------------------DL 604
            .|                                                             .|
Zfish   545 KPRVITVTRTAREIPLPFTLIGGAERGTRLFISSVEVGSKAEEAGLKRGDQILEVNGQTFENVQL 609

  Fly   605 NEETSIVHN-------VLTQM-------ARYQEDRNQNA--------GQK---WKLPPNG----- 639
            ::.|.|:.|       |.|.:       ||..|:|....        |:|   :.:|..|
Zfish   610 SKATEILRNNIQLSMSVKTNLLVFKELVARVAEERKNGVPHLPKIGDGKKNCRYSIPELGAGSDV 674

  Fly   640 ------------------------------------------------------------QPICL 644
                                                                        .|...
Zfish   675 IGQEKISKKAKAHTVGGRNKLMKILDKTRMSILPQKPYSDLGLGQTQDDSIVGLRQNKQTPPTMP 739

  Fly   645 FSGNATPSKTVIRPD-----------------------DDIIFRVYCADHTYCTLRFPMHTTAEL 686
            .||..:.|    .||                       .|.:.|::.||.....|.....|||..
Zfish   740 VSGTLSSS----NPDLAQGQQRIIDYSTQPPAPGFTQLQDQVLRIFKADQQSRYLLANRDTTARE 800

  Fly   687 IKACAADKLQLNRGPEDLVLVEVKSNGERSVFKDNDVSIPTGLS-LNGRLFVSVKDHLDA----- 745
            ....|..:..|:..||...|.|| |..:..|.|..  .:|..|| |..|:.:|.:.:|.:
Zfish   801 AANLAIKEFGLSASPEAFSLCEV-SVTQEGVIKQR--RLPDQLSKLADRIQLSARYYLKSNMETE 862

  Fly   746 --LTQLQEQECPTEGVDIDLEILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITANL 808
              .:.::..|...|.| :.|..||:.|:|..::...:.||..:...:.:...| :.|..:...||
Zfish   863 TLCSDVEALEMQRESV-VPLLSLSSMEIANQLSARNYLLFSCIEPTDYINDLF-KLHPQEPPTNL 925

  Fly   809 DVFLRRFNEVQYWIVTELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRL 873
            ..|....|:..:|:.||:|...:|:|||.:::.|||:|.:|::.:|.|:.||::.|.:...||||
Zfish   926 RNFEGLVNQETFWVATEIVQETNLAKRVKIIKHFIKIALHCRDCKNFNSMFAIISGFNLAPVSRL 990

  Fly   874 QQTWEKIPSKFRKIFQEFEALIDPSRNHRAYRVFVGK--LQPPLIPFMPLLLKDMTFAHEGNKTS 936
            :.|||::|.|:.|:..|.:.:.|||||...||..:.|  ||||:||..|::.||:||.||||.:.
Zfish   991 RSTWERLPGKYEKLLAELQDVFDPSRNMAKYRNLLNKHNLQPPVIPLFPVIKKDLTFLHEGNNSE 1055

  Fly   937 LDGLVNFEKMHMMAQTMRTIRFCRSRSLGLEP 968
            :||||||||:.|:|:.:|.:  .|..|:.::|
Zfish  1056 VDGLVNFEKLRMIAREIRHV--VRMASITMDP 1085

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	26/136 (19%)
		CAP_ED	36..146	CDD:237999	23/128 (18%)
		DEP_Epac	199..330	CDD:239884	19/141 (13%)
		CAP_ED	352..463	CDD:237999	35/110 (32%)
		Crp	373..525	CDD:223736	47/157 (30%)
		RasGEF_N	489..594	CDD:279012	29/126 (23%)
		UBQ	665..>714	CDD:294102	15/48 (31%)
		RasGEF	766..1002	CDD:214539	78/205 (38%)
rapgef2a	XP_005170987.2	CAP_ED	42..148	CDD:237999	21/119 (18%)
		cNMP	299..414	CDD:197516	37/115 (32%)
		RasGEFN	430..543	CDD:214571	31/140 (22%)
		PDZ_signaling	546..627	CDD:238492	5/80 (6%)
		UBQ	775..858	CDD:320785	25/85 (29%)
		RasGEF	881..1085	CDD:238087	78/206 (38%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910