Protein Alignment Epac and rapgef6

DIOPT Version :10

• Sequence 1: NP_001097202.2
• Gene: Epac / 35588
• FlyBaseID: FBgn0085421
• Length: 1006
• Species: Drosophila melanogaster
• Sequence 2: NP_001361718.1
• Gene: rapgef6 / 733941
• XenbaseID: XB-GENE-981546
• Length: 1644
• Species: Xenopus tropicalis
• Alignment Length: 1271
• Identity: 293/1271 - (23%)
• Similarity: 485/1271 - (38%)
• Gaps: 407/1271 - (32%)

  Fly     8 AMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGRFWYA 72
            |:.|:|.:|...|::.:...:.|::.|..|....|:.::....||.......||.:....|.||.
 Frog    12 ALRKKPKERTQEDLKTVYSYMHRMDILSHLREHQLRLMSATARYERYSANQVLFCSESLARCWYI 76

  Fly    73 VLGGSLEVRYHAHADADGKSAVTL--CNLG--------------------VGATFGES---VLHD 112
            :|.||:.|          |:::.|  |:.|                    |....||:   :||
 Frog    77 LLSGSVLV----------KNSMFLPPCSFGKQSGGKRGCDCIVLEPSEMIVVENTGENNDGILH- 130

  Fly   113 LPRDSTVVTKTTCELLRVEQQDFRLIWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKRPL 177
              |::::         |..::.:|.|   |:.......|:    ||     .|.:....||  .|
 Frog   131 --RETSI---------RQSRRRYRKI---NQHGERQTITD----NG-----DAASYVLLPT--DL 170

  Fly   178 SPDHPNPALPITETPSPAMNRMGWALRTLLVADN--SSCLKDRKVSGKLIRKCAPGTELVDWLVN 240
            |..|      :|::|.|.:..:..:.....:|.:  ||.|.|
 Frog   171 SKIH------LTDSPHPHVTHVPSSQSGCSIASDSGSSSLSD----------------------- 206

  Fly   241 LSPIVHTRAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQA----EDL 301
                                                  :|:....|.|.....|:|:|    ||
 Frog   207 --------------------------------------IYQATESEMGDVDLTGLPEAAVDSED- 232

  Fly   302 GAANEHIREALSALFQRGPDATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSS 366
            ...:|.|..|...:..|.   .:|..|.|...:||.:::|.:.|.:..:.|..:::.|::|:|.|
 Frog   233 EEEDEDIERASDTMLTRD---IVRECLEKEPTDRTDDDIEQLLEFMHQLPAFVNMTMSVRRKLCS 294

  Fly   367 IFVFEAHAQAGTILFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAAT 431
            :.:||...|||.|:...|.|..|||::|.|.|: :.|..|.:..|..|:.||....:........
 Frog   295 VMIFEVVEQAGAIIIEDGQELDSWYVILNGIVE-ISHSDGRMENLCMGNSFGINPSLETQYMDGV 358

  Fly   432 IVLKENNCHLLRVDKEHFNRILRDVEANTLRLQEHGKDVLV-----LERVAKQRGQHSAFKYTVM 491
            :..|.::|..:.:.::.:.|||..||.||.:::|.|:.|:|     |:|...::|      :.|:
 Frog   359 VKTKADDCQFVCIAQQDYFRILNHVEKNTHKVKEKGEIVMVNEHRELDRSGTRKG------HIVI 417

  Fly   492 SGTPAKMLEHLLETRLGQSVGGMDP-FLDDFLLTHIVFM-PVVQLVDELANYFHCDAHEDAQT-- 552
            .|||.:::.||:|.   .||  :|| :::|||||:..|: ..:::.|:|..:|..|:..|..|
 Frog   418 QGTPERLILHLIEE---HSV--VDPTYIEDFLLTYRTFLSSPLEVGDKLLEWFKDDSRRDKVTRV 477

  Fly   553 ---------------PEDREYIINFKKRVIQFMQKWVMAVRHAAF----------------EEPS 586
                           |....::..|::.:.....|..:.:.:.|.                |.|.
 Frog   478 VLLWVNNHFNDFEGDPAMTRFLEEFERNLENAKMKGHLRLLNIACAAKAKLRQVTLLKPSRESPF 542

  Fly   587 VCD-----------FIE----------------DLAAEV-----------EADPDLNEETSIVHN 613
            ..:           |||                |...||           :|...|...|.:...
 Frog   543 YFNVVGGSDKGFGMFIESVEPGSKATEAGLKRGDQIMEVNGQNFETIVYAKALEILKNNTHLSIT 607

  Fly   614 VLTQMARY--------QEDRN-------------------------------QNAGQKWK----- 634
            |.|.:..:        ||.:|                               ..|.:|.|
 Frog   608 VKTNIFVFKELLNRIGQEKKNGVPHIPKIAEKKNNRYSIPDLPNDVEQMFPRDRASKKMKANTVS 672

  Fly   635 -----------------LPPN----------------GQPICLFSGNATPSKTVI---RPD---- 659
                             |||.                |...|..|....|....:   .||
 Frog   673 GGRNRIRKILDKTRFSILPPKPFSDGHVIQSQDDSIVGTRQCRHSVAIMPIPGTLSSSSPDLLQA 737

  Fly   660 ------------------DDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNRGPEDLVL 706
                              .|.:.|::.||...|.:.....|||:.:...|..:..|....:...|
 Frog   738 TTNILDFSNPTAFGFYYIPDQVIRIFKADQQCCYIIIGKDTTAKEVVIQALHEFSLTGSTDTYSL 802

  Fly   707 VEVKSNGERSVFKDNDVSIPTGLS-------LNGRLFVSVKDHLDALTQLQE---QECPTEGVDI 761
            .||..:.| .|.|..  .:|..||       ||||.:  :|::::..|...:   ||...|.: |
 Frog   803 CEVSVSPE-GVIKQR--RLPDQLSKLADRIQLNGRYY--LKNNMETETLCSDEDAQELLRESL-I 861

  Fly   762 DLEILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKI-----TANLDVFLRRFNEVQYW 821
            .|..|||.|:|..:::.:::||..:...|.:...|      ||     .|:|..|....|:..:|
 Frog   862 SLLQLSTIEVATQLSMRDFELFRNIEPTEYIDDLF------KIDSKTSAAHLKQFEEVINQETFW 920

  Fly   822 IVTELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRK 886
            :.||::..|:..||:.:|:.|||.|.:|:|.:|.|:.||::.||:...|:||:.||||:|||:.|
 Frog   921 VATEILREPNHLKRMKIVKHFIKTALHCRECRNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEK 985

  Fly   887 IFQEFEALIDPSRNHRAYRVFVG--KLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMM 949
            :.::.:.|.|||||...||..:.  .:|||:||..|::.||:||.||||.:.:||||||||:.|:
 Frog   986 LLRDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDITFLHEGNDSKVDGLVNFEKLRMI 1050

  Fly   950 AQTMRTI----------------RFCRSRSLG-LEPPSPKS-----EG----------------- 975
            |:.:|.:                |..|.|||| |...|..|     :|
 Frog  1051 AKEIRHVIRMTSANMDPAVMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGGHKKRARRSSFLNAKK 1115

  Fly   976 ---------EVRSYISSFRVIDNQRVLTAMSQKVEP 1002
                     :|:.|||:..|..::..:..||.:.||
 Frog  1116 LYEDAQMSRKVKQYISNLSVEIDEEKIQLMSLQCEP 1151

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	CAP_ED	36..146	CDD:237999	27/134 (20%)
		DEP_Epac	199..330	CDD:239884	19/136 (14%)
		CAP_ED	352..463	CDD:237999	34/110 (31%)
		RasGEF_N	489..594	CDD:459873	32/166 (19%)
		Ubl1_cv_Nsp3_N-like	652..>722	CDD:475130	18/94 (19%)
		RasGEF	766..1002	CDD:214539	95/290 (33%)
rapgef6	NP_001361718.1	CAP_ED	37..119	CDD:469590	19/91 (21%)
		CAP_ED	280..380	CDD:237999	28/100 (28%)
		RasGEFN	412..525	CDD:214571	28/123 (23%)
		PDZ_RapGEF2_RapGEF6-like	529..611	CDD:467237	12/81 (15%)
		RA_PDZ-GEF1	758..840	CDD:340483	23/86 (27%)
		RasGEF	863..1097	CDD:214539	88/239 (37%)