DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and Rapgef3

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	XP_038935756.1	Gene:	Rapgef3 / 59326	RGDID:	621869	Length:	1003	Species:	Rattus norvegicus

Alignment Length:	1046	Identity:	396/1046 - (37%)
Similarity:	557/1046 - (53%)	Gaps:	144/1046 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly    52 EDLEKGVTLFRAGEQGRFWYAVLGGSLEVRY--HAHADADGKSAVTLCNLGVGATFGESVLHDLP 114
            ||.:.||.:.|:...|| |.:......:|.:  ..|... |.:.|....:|.....|   |.|:.
  Rat     7 EDAKPGVVVGRSSSSGR-WLSTFLPPSQVSWPGENHWQV-GPAVVESPAVGAPQVGG---LPDVV 66

  Fly   115 RDSTVVTKTTCELLRVEQQDFRLIWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKRPLSP 179
            .:.|::......:.|.....::|::|..:                 ||.......|..|....|.
  Rat    67 PEGTLLNMVLKRMHRPRCCSYQLVFEHRR-----------------PSCIQGLRWTPLTNSEGSL 114

  Fly   180 DHPNPALPITETPSPAMNRMGWALRTLLVADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPI 244
            |.   .:.:.:..:..:::.|..|...|:|...:.::|||...:|.|:|..|.||||.::.|...
  Rat   115 DF---RVSLEQATTEHVHKAGKLLYRHLLATYPTLIRDRKYHLRLHRQCCSGRELVDGILALGLG 176

  Fly   245 VHTRAQAAGMWQALLEEGVLAHVNKEQPFKDK-CFLYRFRIDEEGGTAAAGVPQAEDLGAANEHI 308
            ||:|:||.|:.|.||:||.|.||..:..|:|: ...|||...|         ||........|.:
  Rat   177 VHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFYRFPGPE---------PQPAGTHDVEEEL 232

  Fly   309 REALSALFQRGPDATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAH 373
            .||::.|.||||||.|.:.|||...:||.|||:|:||||:||.|::|||.|:||||:::.:||.|
  Rat   233 VEAMALLSQRGPDALLTVALRKSPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPH 297

  Fly   374 AQAGTILFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENN 438
            ::|||:||:|||:|.||||:.||||:||.||||.|.||..|||||:|||:|||||||||:|:|||
  Rat   298 SKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILRENN 362

  Fly   439 CHLLRVDKEHFNRILRDVEANTLRLQEHGKDVLVLERVAKQRGQH-----SAFKYTVMSGTPAKM 498
            ||.|||||:.||||::||||.|:||:||||.||||||.::..|..     ...:||||||||.|:
  Rat   363 CHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGTPEKI 427

  Fly   499 LEHLLETRLGQSVGGMDP---FLDDFLLTHIVFMPVVQLVDELANYFHCDAHEDAQTPEDRE--Y 558
            ||.|||.....| ...||   ||.||||||.||||..||...|.::||.:..|.|...|...  |
  Rat   428 LELLLEAMRPDS-SAHDPTETFLSDFLLTHSVFMPCTQLFAALLHHFHVEPSEPAGGSEQERSTY 491

  Fly   559 IINFKKRVIQFMQKWVMAVRHAAFEEPSVCDFIEDLAAEVEADPDLNEETSIVHNVLTQMARYQE 623
            |.|.::::::.:.:||.........:|....|::.|:..|..|..|:       |:|.:  :|.|
  Rat   492 ICNKRQQILRLVSRWVALYSPMLRSDPVATSFLQKLSDLVSRDTRLS-------NLLRE--QYPE 547

  Fly   624 DRNQ--------NAGQKWKLPPNGQPI---CLFSGNATP-------------------------- 651
            .|..        |...:.|.||:...:   |:|....:|
  Rat   548 RRRHHRLENGCGNVSPQTKAPPHTPELVMCCMFPSVTSPQDAGLDVRDLLLLHVEQSCETEAPEK 612

  Fly   652 -----------------SKTVIRPDDDIIFRVYCADHTYCTLRFPMHTTAELIKACAADKLQLNR 699
                             |...||..|.:.:.:...||:..||..|:..:...:.|..|.:....:
  Rat   613 ARNAPVWFPNHEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTK 677

  Fly   700 GPEDLVLVEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEGVDIDLE 764
            |.   |||:|.|.|:....:.:...:.|.|.||.|:||.....:..||...||..||.|....|:
  Rat   678 GQ---VLVKVNSAGDVVGLQPDARGVATSLGLNERIFVVDPQEVHELTPHPEQLGPTLGSSEMLD 739

  Fly   765 ILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKI-TANLDVFLRRFNEVQYWIVTELVS 828
            ::|.|:||..:|..:|:||..:|:.||:::..|..|...: ||||:.|:|||||:|||:.|||..
  Rat   740 LVSAKDLAGQLTEHDWNLFNRIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCL 804

  Fly   829 TPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIFQEFEA 893
            .|....|..|:||||||||:.||.:|||:||||:.||||.|:|||..|||::|.|.||::...|.
  Rat   805 CPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALER 869

  Fly   894 LIDPSRNHRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTMRTIRF 958
            |:|||.|||.||:.:.||.||:|||||||||||||.||||.|.::.|:|||||.|||:.:|.:..
  Rat   870 LLDPSWNHRVYRLALTKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAVRMLHH 934

  Fly   959 CRSRSLGLEPPSP---------------------------KSEGEVRSYISSFRVIDNQRVLTAM 996
            |||.|..  |.||                           :|.....:|:...:||||||.|:.:
  Rat   935 CRSHSTA--PLSPLRSRVSHIHEDSQASRISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRL 997

  Fly   997 SQKVEP 1002
            |:::||
  Rat   998 SRELEP 1003

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	20/95 (21%)
		CAP_ED	36..146	CDD:237999	20/95 (21%)
		DEP_Epac	199..330	CDD:239884	50/131 (38%)
		CAP_ED	352..463	CDD:237999	75/110 (68%)
		Crp	373..525	CDD:223736	100/159 (63%)
		RasGEF_N	489..594	CDD:279012	43/109 (39%)
		UBQ	665..>714	CDD:294102	13/48 (27%)
		RasGEF	766..1002	CDD:214539	123/263 (47%)
Rapgef3	XP_038935756.1	DEP_Epac	131..254	CDD:239884	50/131 (38%)
		CAP_ED	278..387	CDD:237999	74/108 (69%)
		RasGEF_N	418..527	CDD:395493	43/109 (39%)
		RasGEF	738..1003	CDD:214539	124/266 (47%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	127	1.000	Domainoid score	I5253
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	624	1.000	Inparanoid score	I830
OMA	1	1.010	-	-		QHG49338
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	1	1.000	-	-		oto97037
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	LDO	PTHR23113
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1712
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	11.980