DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and Rapgef4

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	NP_001191094.1	Gene:	Rapgef4 / 56508	MGIID:	1917723	Length:	1011	Species:	Mus musculus

Alignment Length:	1044	Identity:	506/1044 - (48%)
Similarity:	682/1044 - (65%)	Gaps:	90/1044 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 EWITAMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGR 68
            |||..:||||.:|:..||::|..||:.|:...:...:.|:|:.:||:||:||||:||||.|:.|.
Mouse    14 EWIACLDKRPLERSSEDVDIIFTRLKGVKAFEKFHPNLLRQICLCGYYENLEKGITLFRQGDIGT 78

  Fly    69 FWYAVLGGSLEVRY---HAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRV 130
            .|||||.|||:|:.   .:|.|     |||:|.||:|..||||:|.:.||.:|:||:.:.||||:
Mouse    79 NWYAVLAGSLDVKVSETSSHQD-----AVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRI 138

  Fly   131 EQQDFRLIWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKR--PLSPDHP--NPALPITET 191
            ||:||:.:|||.::.|..:     |...:|.....:.....|.|.  ||...|.  .||..||:.
Mouse   139 EQEDFKALWEKYRQYMAGL-----LAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPAHTITKV 198

  Fly   192 PSPAMNRMGWALRTLLVADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPIVHTRAQAAGMWQ 256
            ||..:.|.|..||..:::.....::|||...|..|:|..|||||||::..:..||:|.||.||||
Mouse   199 PSEKILRAGKILRIAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQ 263

  Fly   257 ALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGAANEHIREALSALFQRGPD 321
            .|||:|||.||::|:.|:||...|||..||.   ..|.:|..|:....:|.:::.:..|.|.|||
Mouse   264 VLLEDGVLNHVDQERHFQDKYLFYRFLDDER---EDAPLPTEEEKKECDEELQDTMLLLSQMGPD 325

  Fly   322 ATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQAGTILFNQGDE 386
            |.:|||||||..:||.::||::::||:||.|||||||::||||:.:.:||:||:.||:|||||:|
Mouse   326 AHMRMILRKPPGQRTVDDLEIIYDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEE 390

  Fly   387 GRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNCHLLRVDKEHFNR 451
            |.||||:|||||:|||:|||.|.||..|||||||||:|||||||:|||:|:|||.||||||.|||
Mouse   391 GTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNR 455

  Fly   452 ILRDVEANTLRLQEHGKDVLVLERV------AKQRGQHSAFKYTVMSGTPAKMLEHLLET-RLGQ 509
            |||||||||:||:||.:||||||:|      |.|.......|||||||||.|:|||.||| ||..
Mouse   456 ILRDVEANTVRLKEHDQDVLVLEKVPAGNRAANQGNSQPQQKYTVMSGTPEKILEHFLETIRLEP 520

  Fly   510 SVG-GMDPFLDDFLLTHIVFMPVVQLVDELANYFHCDAHEDAQTPEDR-EYIINFKKRVIQFMQK 572
            |:. ..|..|:||::.|.||||..||...|..::|  |.....|.::| :|.:|.|:|||:.:.:
Mouse   521 SLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYH--AQPSQGTEQERMDYALNNKRRVIRLVLQ 583

  Fly   573 WVMAVRHAAFEEPSVCDFIEDLAAEVEAD-----------PDLNEETSIV----------HNVLT 616
            |.........|:.....|:|:....|..|           |:|.:....:          |.||.
Mouse   584 WAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMAAFKEQLPELEKIVKQISEDAKAPQKKHKVLL 648

  Fly   617 QMARYQEDRNQNAGQKWKLPPNGQPICLFSGNATPSKTVIRPDDDIIFRVYCADHTYCTLRFPMH 681
            |.....::|.|..          ||              ||..|:::|:|||.||||.|:|.|:.
Mouse   649 QQFNTGDERAQKR----------QP--------------IRGSDEVLFKVYCIDHTYTTIRVPVA 689

  Fly   682 TTAELIKACAADKLQLNRGPEDLVLVEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDAL 746
            .:.:.:.:..||||....|   |::|::.|.||:.|.|.||||:.|.|::|||||...::..|:|
Mouse   690 ASVKEVISAVADKLGSGEG---LIIVKMNSGGEKVVLKSNDVSVFTTLTINGRLFACPREQFDSL 751

  Fly   747 TQLQEQECPTEGVDIDLEILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITANLDVF 811
            |.|.|||.||.|.....|::|:|:|||.:|.::|:||..|||.||:|||||||:|.|.|||||:|
Mouse   752 TPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELFNCVHELELIYHTFGRHNFKKTTANLDLF 816

  Fly   812 LRRFNEVQYWIVTELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQT 876
            ||||||:|:|:|||:.....|||||.|::||||:||:||||:|||:|||:||||||:|||||..|
Mouse   817 LRRFNEIQFWVVTEVCLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALT 881

  Fly   877 WEKIPSKFRKIFQEFEALIDPSRNHRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLV 941
            |||:||||:|.:.|||:|:||||||||||:...||:||||||||||:|||||.||||||.:|.||
Mouse   882 WEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLV 946

  Fly   942 NFEKMHMMAQTMRTIRFCRSRSLGLEPPSP------KSEGEVRSYISSFRVIDNQRVLTAMSQKV 1000
            |||||.|:|.|.||:|:.||     :|.:|      |:..:||||:....||||||.|:.||.::
Mouse   947 NFEKMRMIANTARTVRYYRS-----QPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRL 1006

  Fly  1001 EPTR 1004
            ||.|
Mouse  1007 EPRR 1010

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	57/120 (48%)
		CAP_ED	36..146	CDD:237999	55/112 (49%)
		DEP_Epac	199..330	CDD:239884	56/130 (43%)
		CAP_ED	352..463	CDD:237999	83/110 (75%)
		Crp	373..525	CDD:223736	106/159 (67%)
		RasGEF_N	489..594	CDD:279012	43/107 (40%)
		UBQ	665..>714	CDD:294102	18/48 (38%)
		RasGEF	766..1002	CDD:214539	151/241 (63%)
Rapgef4	NP_001191094.1	Crp	38..>158	CDD:223736	58/129 (45%)
		CAP_ED	43..158	CDD:237999	56/124 (45%)
		DEP_Epac	206..334	CDD:239884	56/130 (43%)
		Crp	351..>458	CDD:223736	78/106 (74%)
		CAP_ED	356..467	CDD:237999	83/110 (75%)
		RasGEF_N	499..605	CDD:279012	43/107 (40%)
		RasGEF	768..1009	CDD:214539	153/245 (62%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	275	1.000	Domainoid score	I1749
eggNOG	1	0.900	-	-		E1_KOG3629
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4451
Inparanoid	1	1.050	942	1.000	Inparanoid score	I346
Isobase	1	0.950	-	0.878984	Normalized mean entropy	S3294
OMA	1	1.010	-	-		QHG49338
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	1	1.000	-	-		oto93498
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	O	PTHR23113
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2054
SonicParanoid	1	1.000	-	-		X1712
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.820