DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Epac and rapgef4a

DIOPT Version :9

Sequence 1:	NP_001097202.2	Gene:	Epac / 35588	FlyBaseID:	FBgn0085421	Length:	1006	Species:	Drosophila melanogaster
Sequence 2:	XP_021334493.1	Gene:	rapgef4a / 559170	ZFINID:	ZDB-GENE-070912-385	Length:	1025	Species:	Danio rerio

Alignment Length:	1028	Identity:	507/1028 - (49%)
Similarity:	686/1028 - (66%)	Gaps:	50/1028 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 EWITAMDKRPCDRNLRDVELISCRLRRVEPLCRLPGSALQQLAMCGFYEDLEKGVTLFRAGEQGR 68
            :||..:||||.:|:..||::|..||:.|:...:.....|||:.:|||||.||||:||:|.|:.|.
Zfish    20 DWIVCLDKRPEERSGEDVDIIMVRLKSVKAFEKFHPPLLQQICLCGFYECLEKGITLYRQGDIGT 84

  Fly    69 FWYAVLGGSLEVRYHAHADADGKSAVTLCNLGVGATFGESVLHDLPRDSTVVTKTTCELLRVEQQ 133
            .||.||.|||:|:  ....|:.:.|||:|.||:|..||||:|.:.||.:|:||:...||||:||:
Zfish    85 NWYTVLSGSLDVK--VSETANHQDAVTICTLGIGTAFGESILDNTPRHATIVTREFSELLRIEQK 147

  Fly   134 DFRLIWEKNKELMNDIFTNCKLKNGFGPSVQATAAATSPTKRPLSPDHP--------------NP 184
            |||.:||..::.|..:     |...:|.......|...|.|..||...|              ||
Zfish   148 DFRNLWENYRQCMAGL-----LVPPYGVMDSGPNADRMPDKENLSNSSPGLSPKHLNKKSFIENP 207

  Fly   185 ----ALPITETPSPAMNRMGWALRTLLVADNSSCLKDRKVSGKLIRKCAPGTELVDWLVNLSPIV 245
                ..||.:.||..:.|.|..||.::::.....::|||...|..|:...||||||||:..:..|
Zfish   208 IKVHPKPINQVPSEKILRAGKVLRNIILSRAPHMIRDRKYHLKTYRRSCIGTELVDWLLQHTSCV 272

  Fly   246 HTRAQAAGMWQALLEEGVLAHVNKEQPFKDKCFLYRFRIDEEGGTAAAGVPQAEDLGAANEHIRE 310
            |:|.||.||||.|||||||.||::|..|:||...|||..||   :..:|:|..||...::|.:::
Zfish   273 HSRVQAVGMWQVLLEEGVLNHVDQEPTFQDKYLFYRFLDDE---SEDSGMPSEEDKKESDEELQD 334

  Fly   311 ALSALFQRGPDATLRMILRKPSHERTSEELELVFEELVHIAALSHLSTSIKRELSSIFVFEAHAQ 375
            .|..|.|.||||.|||||||...:||.::||:::|||:||.|||||||::||||:.:.:||:||:
Zfish   335 TLLLLSQIGPDAHLRMILRKQPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAK 399

  Fly   376 AGTILFNQGDEGRSWYILLKGSVDVVIHGKGTVATLKTGDDFGKLALINDAPRAATIVLKENNCH 440
            |||:|||||:||.||||:|||||:|||:|||.|.||..|||||||||:|||||||:|||:|:.||
Zfish   400 AGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDFCH 464

  Fly   441 LLRVDKEHFNRILRDVEANTLRLQEHGKDVLVLERVAKQRGQHSAFKYTVMSGTPAKMLEHLLET 505
            .||||||.||||||||||||:||:||.:||||||:...:....|..|||||||||.|:|||||||
Zfish   465 FLRVDKEDFNRILRDVEANTVRLKEHEEDVLVLEKANNRSSSSSPLKYTVMSGTPEKILEHLLET 529

  Fly   506 -RLGQSVGGMDPFLDDFLLTHIVFMPVVQLVDELANYFHCDAHEDAQTPEDREYIINFKKRVIQF 569
             :|.......||.||||||.|.||:|..||...|..::|..|.:.:: .|..:|.:|.|:||::.
Zfish   530 MKLDCQFSESDPALDDFLLMHCVFVPNSQLCPLLMTHYHAQASQGSE-QERMDYTLNNKRRVVRL 593

  Fly   570 MQKWVMAVRHAAFEEPSVCDFIEDLAAEVEAD----PDLNEETSIVHNVLTQMARYQEDRNQNAG 630
            :.:|.........||.|...||::....|..|    |.|.:....:..::.|    ..|..:.:.
Zfish   594 VLQWASINTDHLQEEDSSFSFIQEFYEMVCEDSRLTPALKDHLPELEKIIKQ----NSDDGRPSQ 654

  Fly   631 QKWKLPPNGQPICLFS-GN-ATPSKTVIRPDDDIIFRVYCADHTYCTLRFPMHTTAELIKACAAD 693
            :|.|:     .:..|| || ....:..|:..|:|:|:|||:||||.|:|.|:..:...:.|..:|
Zfish   655 KKHKV-----LLRQFSTGNDRLQKRQPIKSTDEILFKVYCSDHTYSTIRVPVSASVREVIAAVSD 714

  Fly   694 KLQLNRGPEDLVLVEVKSNGERSVFKDNDVSIPTGLSLNGRLFVSVKDHLDALTQLQEQECPTEG 758
            ||.   ..:||:|:.:.|.|::.:.|.:||||.:.||:||||||..:|.:|:||.|.|||.|:.|
Zfish   715 KLS---SGDDLLLIHLNSAGDKELLKPSDVSIFSSLSINGRLFVCPRDQIDSLTPLPEQEGPSAG 776

  Fly   759 VDIDLEILSTKELAYHITLFEWDLFWAVHEYELLYHTFGRHHFGKITANLDVFLRRFNEVQYWIV 823
            .....|::|:|:||:.:||::|:||..|||:||:|||||||||.|.|||||:|||||||||.|:|
Zfish   777 SMSTFELMSSKDLAHQMTLYDWELFHCVHEHELIYHTFGRHHFKKTTANLDLFLRRFNEVQLWVV 841

  Fly   824 TELVSTPSLSKRVGLVRKFIKLAAYCKEYQNLNAFFAVVMGLSNMAVSRLQQTWEKIPSKFRKIF 888
            ||:...|:|||||.|::||||:||:|||::|||:|||::|||.|.||.||.|||||:||||:|.:
Zfish   842 TEVCLCPALSKRVQLLKKFIKIAAHCKEFKNLNSFFAIIMGLGNPAVCRLSQTWEKLPSKFKKFY 906

  Fly   889 QEFEALIDPSRNHRAYRVFVGKLQPPLIPFMPLLLKDMTFAHEGNKTSLDGLVNFEKMHMMAQTM 953
            .|||.|:||||||||||:.:.||:||:|||||||:|||||.||||||..|.|||||||.|:|.|:
Zfish   907 GEFENLMDPSRNHRAYRLTMAKLEPPIIPFMPLLIKDMTFTHEGNKTFTDRLVNFEKMRMIANTV 971

  Fly   954 RTIRFCRSRSLGLEPP--SPKSEGEVRSYISSFRVIDNQRVLTAMSQKVEPTR 1004
            |.||:|||:....|.|  :.||..:||:|:....||||||.|:.:|.::||.|
Zfish   972 RIIRYCRSQPFDQEAPQATGKSHQDVRTYVRHISVIDNQRSLSQLSHRLEPRR 1024

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Epac	NP_001097202.2	Crp	28..>146	CDD:223736	56/117 (48%)
		CAP_ED	36..146	CDD:237999	54/109 (50%)
		DEP_Epac	199..330	CDD:239884	60/130 (46%)
		CAP_ED	352..463	CDD:237999	83/110 (75%)
		Crp	373..525	CDD:223736	107/152 (70%)
		RasGEF_N	489..594	CDD:279012	45/105 (43%)
		UBQ	665..>714	CDD:294102	18/48 (38%)
		RasGEF	766..1002	CDD:214539	150/237 (63%)
rapgef4a	XP_021334493.1	CAP_ED	49..158	CDD:237999	54/110 (49%)
		DEP_Epac	226..354	CDD:239884	60/130 (46%)
		CAP_ED	376..487	CDD:237999	83/110 (75%)
		RasGEF_N	513..618	CDD:306971	45/105 (43%)
		RasGEF	781..1023	CDD:214539	152/241 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	275	1.000	Domainoid score	I1719
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4451
Inparanoid	1	1.050	941	1.000	Inparanoid score	I334
OMA	1	1.010	-	-		QHG49338
OrthoDB	1	1.010	-	-		D143470at2759
OrthoFinder	1	1.000	-	-		FOG0000899
OrthoInspector	1	1.000	-	-		otm25598
orthoMCL	1	0.900	-	-		OOG6_103967
Panther	1	1.100	-	-	O	PTHR23113
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1712
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.980